Phenotypes associated with this allele

• Allele Symbol: Tg(Grik4-cre)G32-4Stl
• Allele Name: transgene insertion G32-4, Susumu Tonegawa
• Allele ID: MGI:2387441
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Chrm1^tm2.1Stl/Chrm1^tm2.1Stl Tg(Grik4-cre)G32-4Stl/0	involves: 129P2/OlaHsd * C57BL/6	MGI:4437233
cn2	Camk2a^tm1Vyb/Camk2a^tm1Vyb Tg(Grik4-cre)G32-4Stl/0	involves: C57BL/6	MGI:2655692
cn3	Grin1^tm2Stl/Grin1^tm2Stl Tg(Grik4-cre)G32-4Stl/0	involves: C57BL/6	MGI:3582225

Genotype
MGI:4437233

cn1

• Allelic Composition: Chrm1^tm2.1Stl/Chrm1^tm2.1Stl; Tg(Grik4-cre)G32-4Stl/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal excitatory postsynaptic currents ( J:156540 )

• excitatory postsynaptic currents from hippocampal slices are higher than in wild-type

abnormal long-term depression ( J:156540 )

• (S)-3,5-dihydroxyphenylglycine hydrate (DHPG), an mGluR agonist, fails to produce long-term depression in hippocampal slices

• pretreatment of hippocampal slices with PKC activator 12-O-tetradecanoylphorbol 13-acetate, restores DHPG long-term

decreased paired-pulse facilitation ( J:156540 )

Genotype
MGI:2655692

cn2

• Allelic Composition: Camk2a^tm1Vyb/Camk2a^tm1Vyb; Tg(Grik4-cre)G32-4Stl/0
• Genetic Background: involves: C57BL/6

nervous system

abnormal synaptic transmission ( J:82744 )

• mutants exhibit normal basal neurotransmitter release at CA3-CA1 synapses but show an enhanced activity-dependent increase in the probability of release during repetitive presynaptic stimulation

abnormal CNS synaptic transmission ( J:82744 )

• although synaptic depression is normal, the initial facilitation of the EPSC amplitude during repetitive stimulation of synaptic inputs (frequency facilitation) is significantly enhanced

Genotype
MGI:3582225

cn3

• Allelic Composition: Grin1^tm2Stl/Grin1^tm2Stl; Tg(Grik4-cre)G32-4Stl/0
• Genetic Background: involves: C57BL/6

behavior/neurological

abnormal spatial learning ( J:77718 )

• impaired spatial memory recall after partial cue removal but not under full cue conditions in the Morris water maze and mutants exhibited normal motivation, motor coordination and sensory functions

nervous system

abnormal single cell response ( J:77718 )

• decreased firing rate of putative CA1 interneurons

• CA1 pyramidal cells showed a significant decrease in complex spike bursting properties (complex spike index and burst spike frequency) when mutants were engaged in open-field foraging

• CA1 cells showed significant reductions in burst spike frequency, place field size and integrated firing rate after partial cue removal, however showed no significant changes when mutants were returned to an environment with all the distal cues present

abnormal NMDA-mediated synaptic currents ( J:77718 )

• the 6-cyano-7-dinitroquinoxalline-2,3-dione (DNQX)-insensitive component of the NMDA current was significantly reduced at the recurrent commissural/associational synapse relative to controls

reduced long-term potentiation ( J:77718 )

• long term potentiation was nearly absent at the commissural/associational (CA)-CA3 synapses in 9/11 mutants