Phenotypes associated with this allele

• Allele Symbol: Catsper1^tm1Clph
• Allele Name: targeted mutation 1, David E Clapham
• Allele ID: MGI:2387461
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Catsper1^tm1Clph/Catsper1^tm1Clph	involves: 129S4/SvJae	MGI:3694476
hm2	Catsper1^tm1Clph/Catsper1^tm1Clph	involves: 129S4/SvJae * C57BL/6J	MGI:2450131
cx3	Catsper1^tm1Clph/Catsper1^tm1Clph Lrrc52^tm1.1(KOMP)Vlcg/Lrrc52^tm1.1(KOMP)Vlcg	involves: 129S4/SvJae * C57BL/6NTac	MGI:5896618

Genotype
MGI:3694476

hm1

• Allelic Composition: Catsper1^tm1Clph/Catsper1^tm1Clph
• Genetic Background: involves: 129S4/SvJae

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

reproductive system phenotype ( J:226539 )

♂N • sperm midpiece is not rigid as in Ppp3cc and Ppp3r2 sperm

abnormal sperm physiology ( J:116406 )

♂ • calcium-selective pH sensitive sperm current (I_CatSper) is not detectable

abnormal sperm motility ( J:116406 )

♂ • percent motility of mutant sperm decreases more rapidly than wild-type

asthenozoospermia ( J:116406 )

♂ • after 90 minutes in viscous conditions, sperm lose their motility compared to wild-type which move more vigorously than initially

cellular

abnormal sperm motility ( J:116406 )

♂ • percent motility of mutant sperm decreases more rapidly than wild-type

asthenozoospermia ( J:116406 )

♂ • after 90 minutes in viscous conditions, sperm lose their motility compared to wild-type which move more vigorously than initially

Genotype
MGI:2450131

hm2

• Allelic Composition: Catsper1^tm1Clph/Catsper1^tm1Clph
• Genetic Background: involves: 129S4/SvJae * C57BL/6J

reproductive system

male infertility ( J:72046 )

♂ • male homozygotes exhibit normal mounting behavior but are sterile

♂ • however, mutant males display no significant differences in body and testis weights, spermatogenesis, sperm morphology or sperm counts relative to wild-type littermates

impaired fertilization ( J:72046 )

• in vitro, mutant sperm are unable to fertilize eggs with an intact zona pellucida

• in contrast, mutant sperm are able to fertilize eggs in which the zona pellucida has been removed

impaired sperm penetration of zona pellucida ( J:72046 )

♂ • in vitro, mutant sperm are unable to penetrate the zona pellucida

abnormal sperm physiology ( J:72046 )

♂ • neither cAMP nor cGMP is shown to elicit a significant Ca²⁺ influx in fully differentiated mutant sperm

♂ • in contrast, progesterone, which is used as a positive control for Ca²⁺ responses, initiates a large Ca²⁺ increase in both mutant and wild-type sperm

asthenozoospermia ( J:72046 )

♂ • mutant sperm are sluggish, exhibit less directed movements, and lack the vigorous beating and bending in the tail region noted in wild-type sperm

♂ • major motility parameters, including path velocity, progressive velocity, and track speed, are significantly impaired

homeostasis/metabolism

abnormal calcium ion homeostasis ( J:72046 )

• the cyclic-AMP-induced Ca²⁺ entry in the sperm tail is abolished in mutant sperm

cellular

asthenozoospermia ( J:72046 )

♂ • mutant sperm are sluggish, exhibit less directed movements, and lack the vigorous beating and bending in the tail region noted in wild-type sperm

♂ • major motility parameters, including path velocity, progressive velocity, and track speed, are significantly impaired

Genotype
MGI:5896618

cx3

• Allelic Composition: Catsper1^tm1Clph/Catsper1^tm1Clph; Lrrc52^{tm1.1(KOMP)Vlcg}/Lrrc52^{tm1.1(KOMP)Vlcg}
• Genetic Background: involves: 129S4/SvJae * C57BL/6NTac

reproductive system

abnormal sperm physiology ( J:220171 )

♂ • maximal peak conductances measured at +200 mV at pH 8 are 6.1+/- 0.7 nS