Phenotypes associated with this allele

• Allele Symbol: Rps6^tm1Gtho
• Allele Name: targeted mutation 1, George Thomas
• Allele ID: MGI:2387471
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Rps6^tm1Gtho/Rps6^+ Trp53^tm1Tyj/Trp53^+ Tg(KRT5-cre)5132Jlj/0	involves: 129 * C57BL/6J * DBA/2J	MGI:6260013
cn2	Rps6^tm1Gtho/Rps6^+ Trp53^tm1Tyj/Trp53^tm1Tyj Tg(KRT5-cre)5132Jlj/0	involves: 129 * C57BL/6J * DBA/2J	MGI:6260011
cn3	Rps6^tm1Gtho/Rps6^tm1Gtho Tg(Mx1-cre)1Cgn/0	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:3041081
cn4	Rps6^tm1Gtho/Rps6^+ Tg(Mitf-cre)7114Gsb/0	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:6260006
cn5	Rps6^tm1Gtho/Rps6^+ Tg(KRT5-cre)5132Jlj/0	involves: 129P2/OlaHsd * C57BL/6J * DBA/2J	MGI:6260003

Genotype
MGI:6260013

cn1

• Allelic Composition: Rps6^tm1Gtho/Rps6⁺; Trp53^tm1Tyj/Trp53⁺; Tg(KRT5-cre)5132Jlj/0
• Genetic Background: involves: 129 * C57BL/6J * DBA/2J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

pigmentation

increased foot pad pigmentation ( J:139244 )

• mice exhibit an intermediate footpad pigmentation phenotype and reduced Kitl mRNA levels in the footpad epidermis at P30, indicating partial amelioration of the dark skin observed in mice that are only heterozygous for Rps6^tm1Gtho and hemizygous for Tg(KRT5-cre)5132Jlj

integument

increased foot pad pigmentation ( J:139244 )

• mice exhibit an intermediate footpad pigmentation phenotype and reduced Kitl mRNA levels in the footpad epidermis at P30, indicating partial amelioration of the dark skin observed in mice that are only heterozygous for Rps6^tm1Gtho and hemizygous for Tg(KRT5-cre)5132Jlj

limbs/digits/tail

increased foot pad pigmentation ( J:139244 )

• mice exhibit an intermediate footpad pigmentation phenotype and reduced Kitl mRNA levels in the footpad epidermis at P30, indicating partial amelioration of the dark skin observed in mice that are only heterozygous for Rps6^tm1Gtho and hemizygous for Tg(KRT5-cre)5132Jlj

Genotype
MGI:6260011

cn2

• Allelic Composition: Rps6^tm1Gtho/Rps6⁺; Trp53^tm1Tyj/Trp53^tm1Tyj; Tg(KRT5-cre)5132Jlj/0
• Genetic Background: involves: 129 * C57BL/6J * DBA/2J

pigmentation

pigmentation phenotype ( J:139244 )

N • mice exhibit normal footpad pigmentation and normal Kitl mRNA levels in the footpad epidermis at P30, indicating complete reversal of the phenotype observed in mice that are only heterozygous for Rps6^tm1Gtho and hemizygous for Tg(KRT5-cre)5132Jlj

Genotype
MGI:3041081

cn3

• Allelic Composition: Rps6^tm1Gtho/Rps6^tm1Gtho; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

cellular

abnormal cell morphology ( J:62795 )

• mice induced with interferon gamma exhibited similar responses to starvation as controls, although it was noted that 40S ribosomes were absent and 60S ribosomes were increased in number in mutant liver recovering from starvation

abnormal cell cycle ( J:62795 )

• expression of cell cycle markers such as cyclin E and cyclin A were not seen in mutant hepatocytes suggesting a block in cell cycle progression

liver/biliary system

impaired liver regeneration ( J:62795 )

• after partial hepatectomy, mutant liver failed to regenerate after 10 days; histopathology revealed no evidence of regenerative or pathological processes in mutant liver; expression of cell cycle markers such as cyclin E and cyclin A were not seen in mutants suggesting a block in cell cycle progression

Genotype
MGI:6260006

cn4

• Allelic Composition: Rps6^tm1Gtho/Rps6⁺; Tg(Mitf-cre)7114Gsb/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

pigmentation

abnormal dermal pigmentation ( J:139244 )

• mice exhibit slightly decreased dermal pigmentation relative to control mice

abnormal epidermal pigmentation ( J:139244 )

• mice exhibit decreased epidermal pigmentation relative to control mice

reduced foot pad pigmentation ( J:139244 )

decreased tail pigmentation ( J:139244 )

decreased skin pigmentation ( J:139244 )

• mice exhibit lighter skin than control mice; both the epidermis and, to a lesser extent, the dermis are affected

integument

abnormal dermal pigmentation ( J:139244 )

• mice exhibit slightly decreased dermal pigmentation relative to control mice

abnormal epidermal pigmentation ( J:139244 )

• mice exhibit decreased epidermal pigmentation relative to control mice

reduced foot pad pigmentation ( J:139244 )

decreased tail pigmentation ( J:139244 )

decreased skin pigmentation ( J:139244 )

• mice exhibit lighter skin than control mice; both the epidermis and, to a lesser extent, the dermis are affected

limbs/digits/tail

reduced foot pad pigmentation ( J:139244 )

decreased tail pigmentation ( J:139244 )

Genotype
MGI:6260003

cn5

• Allelic Composition: Rps6^tm1Gtho/Rps6⁺; Tg(KRT5-cre)5132Jlj/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J * DBA/2J

pigmentation

increased ear pigmentation ( J:139244 )

• mice exhibit markedly darkened ears relative to control mice

darkened coat color ( J:139244 )

• mice exhibit markedly darkened hair relative to control mice

abnormal epidermal pigmentation ( J:139244 )

• mice show increased epidermal pigmentation in the footpads, tail and ears

• skin is much darker than that observed in single Rps19^Mhdadsk3 or Rps20^Mhdadsk4 heterozygotes

increased foot pad pigmentation ( J:139244 )

• mice exhibit markedly darkened footpads relative to control mice

• Kitl (kit ligand) mRNA levels are increased 8.2- and 3.8-fold at P3 and P30, respectively, in the footpad epidermis relative to control mice

• i.p. injection of ACK2 (a Kit-neutralizing antibody) at P2 prevents the appearance of dark footpads at P8 whereas PBS injection has no effect on pigmentation, indicating that Kit signaling is required for dark skin

• at P30, a 73-fold increase in Trp53 staining is noted in the basal layer of the footpad epidermis relative to control mice

increased tail pigmentation ( J:139244 )

• mice exhibit markedly darkened tails relative to control mice

• pigment accumulates in the tail epidermis, whereas the dermis is unaffected

integument

increased ear pigmentation ( J:139244 )

• mice exhibit markedly darkened ears relative to control mice

darkened coat color ( J:139244 )

• mice exhibit markedly darkened hair relative to control mice

abnormal epidermal pigmentation ( J:139244 )

• mice show increased epidermal pigmentation in the footpads, tail and ears

• skin is much darker than that observed in single Rps19^Mhdadsk3 or Rps20^Mhdadsk4 heterozygotes

increased foot pad pigmentation ( J:139244 )

• mice exhibit markedly darkened footpads relative to control mice

• Kitl (kit ligand) mRNA levels are increased 8.2- and 3.8-fold at P3 and P30, respectively, in the footpad epidermis relative to control mice

• i.p. injection of ACK2 (a Kit-neutralizing antibody) at P2 prevents the appearance of dark footpads at P8 whereas PBS injection has no effect on pigmentation, indicating that Kit signaling is required for dark skin

• at P30, a 73-fold increase in Trp53 staining is noted in the basal layer of the footpad epidermis relative to control mice

increased tail pigmentation ( J:139244 )

• mice exhibit markedly darkened tails relative to control mice

• pigment accumulates in the tail epidermis, whereas the dermis is unaffected

limbs/digits/tail

increased foot pad pigmentation ( J:139244 )

• mice exhibit markedly darkened footpads relative to control mice

• Kitl (kit ligand) mRNA levels are increased 8.2- and 3.8-fold at P3 and P30, respectively, in the footpad epidermis relative to control mice

• i.p. injection of ACK2 (a Kit-neutralizing antibody) at P2 prevents the appearance of dark footpads at P8 whereas PBS injection has no effect on pigmentation, indicating that Kit signaling is required for dark skin

• at P30, a 73-fold increase in Trp53 staining is noted in the basal layer of the footpad epidermis relative to control mice

increased tail pigmentation ( J:139244 )

• mice exhibit markedly darkened tails relative to control mice

• pigment accumulates in the tail epidermis, whereas the dermis is unaffected

craniofacial

increased ear pigmentation ( J:139244 )

• mice exhibit markedly darkened ears relative to control mice

hearing/vestibular/ear

increased ear pigmentation ( J:139244 )

• mice exhibit markedly darkened ears relative to control mice

growth/size/body

increased ear pigmentation ( J:139244 )

• mice exhibit markedly darkened ears relative to control mice