Phenotypes associated with this allele

• Allele Symbol: Vcam1^tm2Roml
• Allele Name: targeted mutation 2, Mark A Labow
• Allele ID: MGI:2387553
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Vcam1^tm2Roml/Vcam1^tm2Roml	involves: 129S1/Sv * C57BL/6	MGI:3841673
cn2	Vcam1^tm2Roml/Vcam1^tm2Roml Tg(Mx1-cre)1Cgn/?	involves: 129S1/Sv * C57BL/6 * CBA	MGI:3841674

Genotype
MGI:3841673

hm1

• Allelic Composition: Vcam1^tm2Roml/Vcam1^tm2Roml
• Genetic Background: involves: 129S1/Sv * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

postnatal lethality ( J:43561 )

• homozygotes are recovered in somewhat less than expected numbers (16%) in the F1 generation from heterozygote crosses

reproductive system

reproductive system phenotype ( J:43561 )

N • mice appear normal, are fertile and produce normal sized litters

Genotype
MGI:3841674

cn2

• Allelic Composition: Vcam1^tm2Roml/Vcam1^tm2Roml; Tg(Mx1-cre)1Cgn/?
• Genetic Background: involves: 129S1/Sv * C57BL/6 * CBA

immune system

decreased B cell number ( J:68148 )

• phenotype examined after cre activation by IP injection of IFN-alpha

• slightly reduced percentage of B220+ cells in the bone marrow of the femur

decreased transitional stage B cell number ( J:68148 )

• fraction E cells are reduced 40% in bone marrow

• failure to retain immature B cells in bone marrow to complete maturity

decreased mature B cell number ( J:68148 )

• 63% reduction in numbers of fraction F cells in the bone marrow

increased B cell number ( J:68148 )

• phenotype examined after cre activation by IP injection of IFN-alpha

increased immature B cell number ( J:68148 )

• modestly increased in the spleen

increased transitional stage B cell number ( J:68148 )

• dramatically increased number of fraction E cells in the circulation (30% vs 15%)

increased mature B cell number ( J:68148 )

• circulating fraction F cells elevated about 3 fold

abnormal humoral immune response ( J:68148 )

• phenotype examined after cre activation by IP injection of IFN-alpha

• impaired primary and 5 fold reduced secondary IgG1 response to the T cell dependent antigen NP-CG

• response to T-cell independent antigens is normal

hematopoietic system

decreased B cell number ( J:68148 )

• phenotype examined after cre activation by IP injection of IFN-alpha

• slightly reduced percentage of B220+ cells in the bone marrow of the femur

decreased transitional stage B cell number ( J:68148 )

• fraction E cells are reduced 40% in bone marrow

• failure to retain immature B cells in bone marrow to complete maturity

decreased mature B cell number ( J:68148 )

• 63% reduction in numbers of fraction F cells in the bone marrow

increased B cell number ( J:68148 )

• phenotype examined after cre activation by IP injection of IFN-alpha

increased immature B cell number ( J:68148 )

• modestly increased in the spleen

increased transitional stage B cell number ( J:68148 )

• dramatically increased number of fraction E cells in the circulation (30% vs 15%)

increased mature B cell number ( J:68148 )

• circulating fraction F cells elevated about 3 fold