mortality/aging
• embryos become abnormal by E6 and are largely resorbed by E7.5 and E8.5
|
growth/size/body
• some embryos between E6 and E8.5 are significantly smaller than wild-type, however no morphological abnormalities are seen at E5.5
|
embryo
• in the small E6 embryos, endodermal and ectodermal cells are packed loosely and appear not to form the normal columnar ectoderm
|
• some E6.5 embryos have recognizable embryonic tissues but they are not developed appreciably and resemble E6 embryos both in size and degree of germ-layer differentiation
|
• some embryos between E6 and E8.5 are significantly smaller than wild-type, however no morphological abnormalities are seen at E5.5
|
cellular
• blastocyst explants exhibit increased sensitivity to gamma-irradiation, with only the nonmitotic trophoblast giant cells remaining viable after irradiation
|
• E6.5 embryos exhibit a decrease in the number of proliferating cells but no differences in the levels of apoptosis
|