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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Rad50tm1Jpt
targeted mutation 1, John H J Petrini
MGI:2387852
Summary 6 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Rad50tm1Jpt/Rad50tm1Jpt involves: 129S7/SvEvBrd MGI:3615763
ht2
Rad50tm1Jpt/Rad50tm4.1Jpt involves: 129/Sv * 129S7/SvEvBrd * C57BL/6 MGI:5614074
ht3
Rad50tm1Jpt/Rad50tm2Jpt involves: 129S7/SvEvBrd * C57BL/6 MGI:3615661
cn4
Rad50tm1Jpt/Rad50tm3Jpt
Gt(ROSA)26Sortm1(cre/ERT2)Tyj/Gt(ROSA)26Sor+
involves: 129/Sv * C57BL/6 MGI:5614076
cn5
Rad50tm1Jpt/Rad50tm3Jpt
Tg(Pcp2-cre)2Mpin/0
involves: 129S7/SvEvBrd * C57BL/6 MGI:3832543
cn6
Rad50tm1Jpt/Rad50tm3Jpt
Tg(Mx1-cre)1Cgn/0
involves: 129S7/SvEvBrd * C57BL/6 * CBA MGI:3832542


Genotype
MGI:3615763
hm1
Allelic
Composition
Rad50tm1Jpt/Rad50tm1Jpt
Genetic
Background
involves: 129S7/SvEvBrd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rad50tm1Jpt mutation (1 available); any Rad50 mutation (53 available)
phenotype observed in females
phenotype observed in males
N normal phenotype

Development of Rad50tm1Jpt/Rad50tm1Jpt embryos

mortality/aging
• embryos become abnormal by E6 and are largely resorbed by E7.5 and E8.5

growth/size/body
• some embryos between E6 and E8.5 are significantly smaller than wild-type, however no morphological abnormalities are seen at E5.5

embryo
• in the small E6 embryos, endodermal and ectodermal cells are packed loosely and appear not to form the normal columnar ectoderm
• some E6.5 embryos have recognizable embryonic tissues but they are not developed appreciably and resemble E6 embryos both in size and degree of germ-layer differentiation
• some embryos between E6 and E8.5 are significantly smaller than wild-type, however no morphological abnormalities are seen at E5.5

cellular
• blastocyst explants exhibit increased sensitivity to gamma-irradiation, with only the nonmitotic trophoblast giant cells remaining viable after irradiation
• E6.5 embryos exhibit a decrease in the number of proliferating cells but no differences in the levels of apoptosis




Genotype
MGI:5614074
ht2
Allelic
Composition
Rad50tm1Jpt/Rad50tm4.1Jpt
Genetic
Background
involves: 129/Sv * 129S7/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rad50tm1Jpt mutation (1 available); any Rad50 mutation (53 available)
Rad50tm4.1Jpt mutation (0 available); any Rad50 mutation (53 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• no live mice are found at birth




Genotype
MGI:3615661
ht3
Allelic
Composition
Rad50tm1Jpt/Rad50tm2Jpt
Genetic
Background
involves: 129S7/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rad50tm1Jpt mutation (1 available); any Rad50 mutation (53 available)
Rad50tm2Jpt mutation (0 available); any Rad50 mutation (53 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• 42% die by 5 months of age, however survival is increased compared to homozygous Rad50tm2Jpt mice

hematopoietic system
• 12 of 29 die with anemia
• most die from hematopoietic failure

neoplasm
• 3 of 29 die with lymphoma




Genotype
MGI:5614076
cn4
Allelic
Composition
Rad50tm1Jpt/Rad50tm3Jpt
Gt(ROSA)26Sortm1(cre/ERT2)Tyj/Gt(ROSA)26Sor+
Genetic
Background
involves: 129/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1(cre/ERT2)Tyj mutation (3 available); any Gt(ROSA)26Sor mutation (992 available)
Rad50tm1Jpt mutation (1 available); any Rad50 mutation (53 available)
Rad50tm3Jpt mutation (1 available); any Rad50 mutation (53 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• tamoxifen treated mice do not live beyond 2 weeks of age due to intestinal failure

digestive/alimentary system
• intestinal defects are seen by day 9 of tamoxifen treatment
• die of intestinal failure




Genotype
MGI:3832543
cn5
Allelic
Composition
Rad50tm1Jpt/Rad50tm3Jpt
Tg(Pcp2-cre)2Mpin/0
Genetic
Background
involves: 129S7/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rad50tm1Jpt mutation (1 available); any Rad50 mutation (53 available)
Rad50tm3Jpt mutation (1 available); any Rad50 mutation (53 available)
Tg(Pcp2-cre)2Mpin mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
behavior/neurological
• minor

nervous system
N
• mice exhibit normal nervous system morphology with no increase in DNA damage




Genotype
MGI:3832542
cn6
Allelic
Composition
Rad50tm1Jpt/Rad50tm3Jpt
Tg(Mx1-cre)1Cgn/0
Genetic
Background
involves: 129S7/SvEvBrd * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rad50tm1Jpt mutation (1 available); any Rad50 mutation (53 available)
Rad50tm3Jpt mutation (1 available); any Rad50 mutation (53 available)
Tg(Mx1-cre)1Cgn mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
liver/biliary system
• following partial hepatectomy, proliferation of pIpC-treated hepatocyte is decreased compared to in wild-type mice due to accumulation of DNA damage
• however, a subset of hepatocytes resolve their DNA damage and divide
• while liver regeneration following partial hepatectomy is normal, pIpC-treated hepatocytes exhibit an increase in DNA damage compared to wild-type cells
• however, a subset of hepatocytes resolve their DNA damage and divide
• following partial hepatectomy, pIpC-treated livers exhibit reduced cellularity and endoreduplication compared to in similarly-treated wild-type mice

hematopoietic system
• the only viable bone marrow left after treatment with pIpC fails to exhibit recombination

cellular
• following partial hepatectomy, proliferation of pIpC-treated hepatocyte is decreased compared to in wild-type mice due to accumulation of DNA damage
• however, a subset of hepatocytes resolve their DNA damage and divide





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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory