Phenotypes associated with this allele

• Allele Symbol: Brs3^tm1Hoh
• Allele Name: targeted mutation 1, Hiroko Ohki-Hamazaki
• Allele ID: MGI:2387858
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Brs3^tm1Hoh/Brs3^tm1Hoh	involves: 129P2/OlaHsd * C57BL/6J	MGI:2658811

Genotype
MGI:2658811

hm1

• Allelic Composition: Brs3^tm1Hoh/Brs3^tm1Hoh
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

adipose tissue

increased white adipose tissue amount ( J:44242 )

♀ • at 27-34 weeks, mutants exhibited an increased amount of white adipose tissue mass

behavior/neurological

behavior/neurological phenotype ( J:44242 )

♀N • when placed in a cage, mutants did not display a reduction in activity compared to controls during the test period (1 hour)

♀N • mutants showed normal activity levels both during light and dark periods over a 3-week observation period

decreased fluid intake ( J:44242 )

♀ • at 10-12 weeks, food intake was similar to that of wild-type, but water intake was reduced

polyphagia ( J:44242 )

♀ • at 20-24 weeks, while obesity progressed, mutants ingested ~9% more calories than wild-type

♀ • in addition to hyperphagia, mutants exhibited increased feed efficiency: as a result, they displayed a slower rate of weight loss when food was restricted

cardiovascular system

increased systemic arterial blood pressure ( J:44242 )

♀ • at 40 weeks of age, mutants showed increases of 13% and 27% in systolic and diastolic blood pressure, respectively

growth/size/body

obese ( J:44242 )

♀ • beginning at ~16 weeks, mutants (F2 generation) showed a significantly greater weight gain relative to wild-type

♀ • mild obesity was preceded by a reduced metabolic rate

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:44242 )

♀N • mutants showed no defects in body temperature regulation, and displayed normal non-shivering brown-fat thermogenesis

increased circulating leptin level ( J:44242 )

♀ • the level of circulating leptin was elevated by 38% at 10 weeks; at 21-22 weeks it reached a concentration 5x higher than that measured in wild-type

decreased circulating growth hormone level ( J:44242 )

♀ • in mutants, the secretion of growth hormone was diminished by 46%; in contrast, blood contents of thyroid hormone and glucagon appeared unaffected

decreased oxygen consumption ( J:44242 )

♀ • at 6-7 weeks, mutants consumed 11% less oxygen than wild-type in the resting state

abnormal glucose homeostasis ( J:44242 )

♀ • following oral glucose administration, 26-week-old mutants showed higher levels of blood glucose and insulin relative to wild-type

♀ • following insulin injection, mutants were unable to clear glucose as efficiently as wild-type

increased circulating insulin level ( J:44242 )

♀ • following a 24 hr fasting period, the blood glucose, cholesterol and triacyl glycerol levels were normal; however, at 23 weeks, the insulin level was elevated significantly relative to wild-type

decreased response to leptin ( J:44242 )

♀ • mutants became obese despite the elevated plasma leptin level, suggesting reduced leptin sensitivity