Phenotypes associated with this allele

• Allele Symbol: Eif2ak4^tm1Drc
• Allele Name: targeted mutation 1, Douglas R Cavener
• Allele ID: MGI:2387965
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Eif2ak4^tm1Drc/Eif2ak4^tm1Drc	B6.129S6-Eif2ak4^tm1Drc	MGI:3688396
hm2	Eif2ak4^tm1Drc/Eif2ak4^tm1Drc	involves: 129S6/SvEvTac * C57BL/6	MGI:3688394
cx3	Eif2ak4^tm1Drc/Eif2ak4^tm1Drc Impact^tm1.1Casb/Impact^tm1.1Casb	involves: 129S6/SvEvTac * C57BL/6J	MGI:6710959

Genotype
MGI:3688396

hm1

• Allelic Composition: Eif2ak4^tm1Drc/Eif2ak4^tm1Drc
• Genetic Background: B6.129S6-Eif2ak4^tm1Drc

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

increased susceptibility to induced morbidity/mortality ( J:92545 )

• Background Sensitivity: substantial mortality when fed a leucine deficient diet

postnatal lethality, incomplete penetrance ( J:92545 )

• some litters die within 1-2 days of birth

reproductive system

increased litter size ( J:92545 )

• Background Sensitivity: only slightly larger than average litter size on a normal diet but smaller than when the strain background is more mixed

growth/size/body

growth/size/body region phenotype ( J:92545 )

N • Background Sensitivity: grow more like controls until weaning after which time growth reaches a plateau

postnatal growth retardation ( J:92545 )

• Background Sensitivity: weaning at 28 days

behavior/neurological

behavior/neurological phenotype ( J:92545 )

N • Background Sensitivity: drop in food consumption on a leucine deficient diet similar to controls

homeostasis/metabolism

abnormal circulating amino acid level ( J:92545 )

• serum amino acid levels are indicative of protein catabolism

liver/biliary system

decreased liver weight ( J:92545 )

• loss of liver weight on a leucine deficient diet is proportionately less than body weight loss, unlike control

muscle

decreased skeletal muscle mass ( J:92545 )

• loss of muscle mass is proportionately greater than overall weight loss on a leucine deficient diet

Genotype
MGI:3688394

hm2

• Allelic Composition: Eif2ak4^tm1Drc/Eif2ak4^tm1Drc
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

Eif2ak4^tm1Drc/Eif2ak4^tm1Drc mice fed a leucine-devoid diet appear scruffy and lethargic

cellular

maternal effect ( J:78945 )

• homozygous females fed a leucine deficient diet show a 3 fold reduction of homozygous progeny

• large numbers of still born pups (24%) when mothers are on a deficient diet

• diets deficient in tryptophan or glycine result in more modest and non significant reductions of homozygous progeny

mortality/aging

mortality/aging ( J:78945 , J:92545 )

N • homozygotes are unexpectedly normal at birth and survive for more than a year (J:78945)

N • Background Sensitivity: mortality on a leucine deficient diet not substantially different than controls (J:92545)

reproductive system

decreased litter size ( J:78945 )

• homozygous females fed a leucine deficient diet show a 3 fold reduction of homozygous progeny

• large numbers of still born pups (24%) when mothers are on a deficient diet

• diets deficient in tryptophan or glycine result in more modest and non significant reductions of homozygous progeny

increased litter size ( J:92545 )

• Background Sensitivity: larger than average litter size on a normal diet

growth/size/body

increased susceptibility to weight loss ( J:92545 )

• Background Sensitivity: body weight loss on a leucine deficient diet greater than in controls

increased growth rate ( J:92545 )

• Background Sensitivity: grow more rapidly than controls to 28 days of age

• Background Sensitivity: weaning at 21 days

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:78945 )

N • liver glycogen levels in adults are unexpectedly normal

abnormal circulating amino acid level ( J:92545 )

• serum amino acid levels are indicative of protein catabolism

liver/biliary system

decreased liver weight ( J:92545 )

• loss of liver weight on a leucine deficient diet is proportionately less than body weight loss, unlike controls

muscle

decreased skeletal muscle mass ( J:92545 )

• loss of muscle mass is proportionately greater than overall weight loss on a leucine deficient diet

behavior/neurological

abnormal eating behavior ( J:92545 )

• Background Sensitivity: greater drop in food consumption than in controls when fed a leucine deficient diet

Genotype
MGI:6710959

cx3

• Allelic Composition: Eif2ak4^tm1Drc/Eif2ak4^tm1Drc; Impact^tm1.1Casb/Impact^tm1.1Casb
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6J

homeostasis/metabolism

decreased susceptibility to diet-induced obesity ( J:279071 )

• maintain a lean phenotype on a high fat diet

decreased body temperature ( J:279071 )

• reduced after 18 hr of fasting but returns to normal after refeeding

• decrease in body temperature induced by fasting is less than in Impact single homozygous mice

growth/size/body

decreased susceptibility to diet-induced obesity ( J:279071 )

• maintain a lean phenotype on a high fat diet