Phenotypes associated with this allele

• Allele Symbol: Tg(tetO-Vegfa)1Kesh
• Allele Name: transgene insertion 1, Eli Keshet
• Allele ID: MGI:2388011
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gt(ROSA)26Sor^tm1(rtTA,EGFP)Nagy/Gt(ROSA)26Sor^+ Sox9^tm3(cre)Crm/Sox9^+ Tg(tetO-Vegfa)1Kesh/0	involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ	MGI:3840959
cx2	Tg(Myh6-tTA)6Smbf/0 Tg(tetO-Vegfa)1Kesh/0	involves: C57BL/6 * CBA	MGI:2665570

Genotype
MGI:3840959

cn1

• Allelic Composition: Gt(ROSA)26Sor^{tm1(rtTA,EGFP)Nagy}/Gt(ROSA)26Sor⁺; Sox9^tm3(cre)Crm/Sox9⁺; Tg(tetO-Vegfa)1Kesh/0
• Genetic Background: involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cardiovascular system

abnormal blood vessel morphology ( J:147285 )

• the vascular network of the limbs is denser and more complex

• metacarpal centers are enriched for thicker vessels originating from the axial artery

• metacarpal centers are wider and split into a denser and more complex network of small capillaries in the interdigital areas

• however, formation of avascular areas is not affected

increased vascular endothelial cell number ( J:147285 )

Genotype
MGI:2665570

cx2

• Allelic Composition: Tg(Myh6-tTA)6Smbf/0; Tg(tetO-Vegfa)1Kesh/0
• Genetic Background: involves: C57BL/6 * CBA

cardiovascular system

abnormal cardiac epithelial to mesenchymal transition ( J:68214 )

• mesenchymal transformation and cellularization of the cardiac jelly are completely blocked in about half of E10.5 embryos

abnormal cardiac outflow tract development ( J:68214 )

• outflow tract development is impaired with cardiac jelly remaining empty by the time it is colonized with mesenchymal cells in controls

abnormal heart development ( J:68214 )

• exhibit abnormal cardiac development upon tetracycline treatment to induce early expression of Vegfa

abnormal endocardium morphology ( J:68214 )

• multi-layered endocardium