Phenotypes associated with this allele

• Allele Symbol: Dlg2^tm1Dsb
• Allele Name: targeted mutation 1, David S Bredt
• Allele ID: MGI:2388036
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Dlg2^tm1Dsb/Dlg2^tm1Dsb	involves: 129/Sv * C57BL/6	MGI:3693953
cx2	Dlg2^tm1Dsb/Dlg2^tm1Dsb Tg(Thy1-EGFP)JJrs/?	involves: 129/Sv * C57BL/6J * CBA	MGI:3693954

Genotype
MGI:3693953

hm1

• Allelic Composition: Dlg2^tm1Dsb/Dlg2^tm1Dsb
• Genetic Background: involves: 129/Sv * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

behavior/neurological phenotype ( J:68924 )

N • generally normal

abnormal touch/ nociception ( J:84686 )

• withdrawl latencies and frequencies in von Frey and high intensity radiant heat tests were similar to controls

unresponsive to tactile stimuli ( J:84686 )

• complete Freund's reagent failed to cause hyppersensitivity to mechanical pain stimuli

• only modest increases in response to mechanical stimulus after 5th lumbar nerve transection and no increase in contralateral withdrawal frequencies

nervous system

abnormal nervous system morphology ( J:68924 , J:84686 )

• gossly normal (J:68924)

• surface expression of NR2A and NR2B N-methyl-D-aspartate (NMDA) receptors is reduced by 64 and 50% respectively on cultured spinal dorsal horn neurons and on the spinal cord itself (J:84686)

abnormal CNS synaptic transmission ( J:84686 )

abnormal excitatory postsynaptic currents ( J:84686 )

• reduced or absent EPSC responses related to NMDA receptors in dorsal horn neurons

abnormal excitatory postsynaptic potential ( J:84686 )

• reduced or absent EPSP responses related to NMDA receptors in dorsal horn neurons

• fEPSP related to NMPA receptors in various regions of the forebrain also reduced or absent

Genotype
MGI:3693954

cx2

• Allelic Composition: Dlg2^tm1Dsb/Dlg2^tm1Dsb; Tg(Thy1-EGFP)JJrs/?
• Genetic Background: involves: 129/Sv * C57BL/6J * CBA

nervous system

abnormal submandibular ganglion morphology ( J:87449 )

• post synaptic receptor clusters were lost more rapidly after denervation of the presynaptic fibers