Phenotypes associated with this allele

• Allele Symbol: Plcg1^tm1Gcrp
• Allele Name: targeted mutation 1, Graham Carpenter
• Allele ID: MGI:2388141
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Plcg1^tm1Gcrp/Plcg1^tm1Gcrp	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:3629732
cn2	Plcg1^tm1Gcrp/Plcg1^tm1Rwen Tg(Cd4-cre)1Cwi/0	either: B6.Cg-Plcg1^tm1Gcrp/Plcg1^tm1Rwen Tg(Cd4-cre)1Cwi or (involves: 129X1/SvJ * C57BL/6 * DBA/2)	MGI:4437917
cn3	Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor^+ Plcg1^tm1Gcrp/Plcg1^tm1Rwen Tg(Cd4-cre)1Cwi/0	involves: 129X1/SvJ * C57BL/6 * DBA/2	MGI:4437914
cn4	Plcg1^tm1Gcrp/Plcg1^tm1Rwen Tg(Cd4-cre)1Cwi/0 Tg(TcraH-Y,TcrbH-Y)71Vbo/0	involves: 129X1/SvJ * C57BL/6 * DBA/2	MGI:4437915

Genotype
MGI:3629732

hm1

• Allelic Composition: Plcg1^tm1Gcrp/Plcg1^tm1Gcrp
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:39281 )

• homozygotes die at ~E9.0-E9.5; embryonic lethality is consistently observed in different genetic backgrounds, including inbred 129X1/SvJ or hybrid backgrounds 129X1/SvJ C57BL/6, 129X1/SvJ Black Swiss, and 129X1/SvJ CD-1

embryo

embryonic growth arrest ( J:39281 )

• homozygotes fail to grow and develop beyond E8.5-E9.0

embryonic growth retardation ( J:39281 )

• at E8.5, ~20% of homozygotes exhibit retarded growth and development; however, placental growth and maternal-fetal connections appear normal

• by E9.5, all homozygotes are significantly retarded in size relative to wild-type embryos

growth/size/body

embryonic growth retardation ( J:39281 )

• at E8.5, ~20% of homozygotes exhibit retarded growth and development; however, placental growth and maternal-fetal connections appear normal

• by E9.5, all homozygotes are significantly retarded in size relative to wild-type embryos

Genotype
MGI:4437917

cn2

• Allelic Composition: Plcg1^tm1Gcrp/Plcg1^tm1Rwen; Tg(Cd4-cre)1Cwi/0
• Genetic Background: either: B6.Cg-Plcg1^tm1Gcrp/Plcg1^tm1Rwen Tg(Cd4-cre)1Cwi or (involves: 129X1/SvJ * C57BL/6 * DBA/2)

Plcg1^tm1Rwen/Plcg1^tm1Gcrp Tg(Cd4-cre)1Cwi/0 mice develop inflammatory/autoimmune disease

immune system

ear inflammation ( J:157757 )

increased CD4-positive, alpha-beta T cell number ( J:157757 )

• in the spleen and lymph nodes compared with Plcg1^tm1Gcrp/Plcg1⁺ Tg(CD4-cre)1Cwi mice

decreased T cell number ( J:157757 )

• peripheral T cells are reduced compared to in wild-type mice

decreased regulatory T cell number ( J:157757 )

• in the thymus and spleen compared with Plcg1^tm1Gcrp/Plcg1⁺ Tg(CD4-cre)1Cwi mice

abnormal T cell activation ( J:157757 )

• mice exhibit an increase in T cell activation compared with wild-type mice

• however, activation phenotype is not cell intrinsic

increased T cell proliferation ( J:157757 )

• mice exhibit a higher percentage of single positive T cells and splenic T cells than in wild-type mice

increased anti-double stranded DNA antibody level ( J:157757 )

• in symptomatic mice without an increase in anti-nuclear antibodies

skin inflammation ( J:157757 )

dermatitis ( J:157757 )

• in one third of mice

growth/size/body

decreased body size ( J:157757 )

• from 2 to 3 weeks

decreased body weight ( J:157757 )

• in female and male mice

digestive/alimentary system

rectal prolapse ( J:157757 )

• in one third of mice

hearing/vestibular/ear

ear inflammation ( J:157757 )

hematopoietic system

increased CD4-positive, alpha-beta T cell number ( J:157757 )

• in the spleen and lymph nodes compared with Plcg1^tm1Gcrp/Plcg1⁺ Tg(CD4-cre)1Cwi mice

decreased T cell number ( J:157757 )

• peripheral T cells are reduced compared to in wild-type mice

decreased regulatory T cell number ( J:157757 )

• in the thymus and spleen compared with Plcg1^tm1Gcrp/Plcg1⁺ Tg(CD4-cre)1Cwi mice

abnormal T cell activation ( J:157757 )

• mice exhibit an increase in T cell activation compared with wild-type mice

• however, activation phenotype is not cell intrinsic

increased T cell proliferation ( J:157757 )

• mice exhibit a higher percentage of single positive T cells and splenic T cells than in wild-type mice

integument

skin inflammation ( J:157757 )

dermatitis ( J:157757 )

• in one third of mice

alopecia ( J:157757 )

• in one third of mice

cellular

increased T cell proliferation ( J:157757 )

• mice exhibit a higher percentage of single positive T cells and splenic T cells than in wild-type mice

Genotype
MGI:4437914

cn3

• Allelic Composition: Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺; Plcg1^tm1Gcrp/Plcg1^tm1Rwen; Tg(Cd4-cre)1Cwi/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6 * DBA/2

immune system

increased T cell apoptosis ( J:157757 )

• following reactivation

abnormal CD4-positive T cell differentiation ( J:157757 )

• differentiation into CD4 or CD8 single positive thymocytes is reduced compared to in wild-type mice

abnormal CD8-positive, alpha-beta T cell differentiation ( J:157757 )

• differentiation into CD4 or CD8 single positive thymocytes is reduced compared to in wild-type mice

increased double-positive T cell number ( J:157757 )

decreased T cell number ( J:157757 )

• peripheral T cells are reduced compared to in wild-type mice

• in transplantation experiments, T cells are less competitive than wild-type T cells

decreased CD4-positive, alpha-beta T cell number ( J:157757 )

• in the thymus

decreased CD8-positive, alpha-beta T cell number ( J:157757 )

• in the thymus

decreased single-positive T cell number ( J:157757 )

• CD4+ thymocytes are more profoundly decreased than CD8+ thymocytes

abnormal T cell activation ( J:157757 )

• mice exhibit an increase in T cell activation compared with wild-type mice

• however, activation phenotype is not cell intrinsic

decreased T cell proliferation ( J:157757 )

• in response to stimulation with anti-CD3m anti-CD3/anti-CD28, or anti-CD3/IL2, T cell proliferation is reduced compared to in similarly treated Plcg1^tm1Gcrp/Plcg1⁺ Tg(CD4-cre)1Cwi Gt(ROSA)26Sor^tm1(EYFP)Cos mice

• however, treatment with PMA/ionomycin restores proliferation

abnormal regulatory T cell physiology ( J:157757 )

• YFP+ T regulatory cells exhibit reduced ability to suppress naive T cell proliferation compared with wild-type T cells

decreased interferon-gamma secretion ( J:157757 )

• following anti-CD3/anti-CD28 stimulation, slightly fewer IFN-gamma producing single positive T cells are detected compared to in similarly treated Plcg1^tm1Gcrp/Plcg1⁺ Tg(CD4-cre)1Cwi Gt(ROSA)26Sor^tm1(EYFP)Cos mice

decreased interleukin-2 secretion ( J:157757 )

• following anti-CD3/anti-CD28 stimulation, IL2 production by single positive T cells is reduced compared to in similarly treated Plcg1^tm1Gcrp/Plcg1⁺ Tg(CD4-cre)1Cwi Gt(ROSA)26Sor^tm1(EYFP)Cos mice

dermatitis ( J:157757 )

• mice exhibit dermatitis unlike wild-type mice

• however, treatment with wild-type T regulatory cells prevents development of symptom

growth/size/body

decreased body weight ( J:157757 )

• mice weight less than wild-type mice

• however, transfer of regulatory T cells restores normal weight gain

digestive/alimentary system

rectal prolapse ( J:157757 )

• mice exhibit rectal prolapse unlike wild-type mice

• however, treatment with wild-type T regulatory cells prevents development of symptom

hematopoietic system

increased T cell apoptosis ( J:157757 )

• following reactivation

abnormal CD4-positive T cell differentiation ( J:157757 )

• differentiation into CD4 or CD8 single positive thymocytes is reduced compared to in wild-type mice

abnormal CD8-positive, alpha-beta T cell differentiation ( J:157757 )

• differentiation into CD4 or CD8 single positive thymocytes is reduced compared to in wild-type mice

increased double-positive T cell number ( J:157757 )

decreased T cell number ( J:157757 )

• peripheral T cells are reduced compared to in wild-type mice

• in transplantation experiments, T cells are less competitive than wild-type T cells

decreased CD4-positive, alpha-beta T cell number ( J:157757 )

• in the thymus

decreased CD8-positive, alpha-beta T cell number ( J:157757 )

• in the thymus

decreased single-positive T cell number ( J:157757 )

• CD4+ thymocytes are more profoundly decreased than CD8+ thymocytes

abnormal T cell activation ( J:157757 )

• mice exhibit an increase in T cell activation compared with wild-type mice

• however, activation phenotype is not cell intrinsic

decreased T cell proliferation ( J:157757 )

• in response to stimulation with anti-CD3m anti-CD3/anti-CD28, or anti-CD3/IL2, T cell proliferation is reduced compared to in similarly treated Plcg1^tm1Gcrp/Plcg1⁺ Tg(CD4-cre)1Cwi Gt(ROSA)26Sor^tm1(EYFP)Cos mice

• however, treatment with PMA/ionomycin restores proliferation

abnormal regulatory T cell physiology ( J:157757 )

• YFP+ T regulatory cells exhibit reduced ability to suppress naive T cell proliferation compared with wild-type T cells

integument

dermatitis ( J:157757 )

• mice exhibit dermatitis unlike wild-type mice

• however, treatment with wild-type T regulatory cells prevents development of symptom

alopecia ( J:157757 )

• mice exhibit alopecia unlike wild-type mice

• however, treatment with wild-type T regulatory cells prevents development of symptom

cellular

increased T cell apoptosis ( J:157757 )

• following reactivation

decreased T cell proliferation ( J:157757 )

• in response to stimulation with anti-CD3m anti-CD3/anti-CD28, or anti-CD3/IL2, T cell proliferation is reduced compared to in similarly treated Plcg1^tm1Gcrp/Plcg1⁺ Tg(CD4-cre)1Cwi Gt(ROSA)26Sor^tm1(EYFP)Cos mice

• however, treatment with PMA/ionomycin restores proliferation

Genotype
MGI:4437915

cn4

• Allelic Composition: Plcg1^tm1Gcrp/Plcg1^tm1Rwen; Tg(Cd4-cre)1Cwi/0; Tg(TcraH-Y,TcrbH-Y)71Vbo/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6 * DBA/2

immune system

thymus hyperplasia ( J:157757 )

• in male mice, which undergo negative selection, compared with Plcg1^tm1Gcrp/Plcg1⁺ Tg(CD4-cre)1Cwi Tg(TcraH-Y,TcrbH-Y)71Vbo mice

decreased double-negative T cell number ( J:157757 )

• in male mice, which undergo negative selection, compared with Plcg1^tm1Gcrp/Plcg1⁺ Tg(CD4-cre)1Cwi Tg(TcraH-Y,TcrbH-Y)71Vbo mice

increased double-positive T cell number ( J:157757 )

• in male mice, which undergo negative selection, compared with Plcg1^tm1Gcrp/Plcg1⁺ Tg(CD4-cre)1Cwi Tg(TcraH-Y,TcrbH-Y)71Vbo mice

decreased CD8-positive, alpha-beta T cell number ( J:157757 )

• female mice, which undergo positive selection, exhibit a decrease in total CD8 single positive cells and CD8 single positive cells in the T3-70^hi cell compartment compared with Plcg1^tm1Gcrp/Plcg1⁺ Tg(CD4-cre)1Cwi Tg(TcraH-Y,TcrbH-Y)71Vbo mice

increased CD8-positive, alpha-beta T cell number ( J:157757 )

• male mice, which undergo negative selection, exhibit an increase in CD8 single positive cells in the T3-70^hi cell compartment compared with Plcg1^tm1Gcrp/Plcg1⁺ Tg(CD4-cre)1Cwi Tg(TcraH-Y,TcrbH-Y)71Vbo mice

hematopoietic system

thymus hyperplasia ( J:157757 )

• in male mice, which undergo negative selection, compared with Plcg1^tm1Gcrp/Plcg1⁺ Tg(CD4-cre)1Cwi Tg(TcraH-Y,TcrbH-Y)71Vbo mice

decreased double-negative T cell number ( J:157757 )

• in male mice, which undergo negative selection, compared with Plcg1^tm1Gcrp/Plcg1⁺ Tg(CD4-cre)1Cwi Tg(TcraH-Y,TcrbH-Y)71Vbo mice

increased double-positive T cell number ( J:157757 )

• in male mice, which undergo negative selection, compared with Plcg1^tm1Gcrp/Plcg1⁺ Tg(CD4-cre)1Cwi Tg(TcraH-Y,TcrbH-Y)71Vbo mice

decreased CD8-positive, alpha-beta T cell number ( J:157757 )

• female mice, which undergo positive selection, exhibit a decrease in total CD8 single positive cells and CD8 single positive cells in the T3-70^hi cell compartment compared with Plcg1^tm1Gcrp/Plcg1⁺ Tg(CD4-cre)1Cwi Tg(TcraH-Y,TcrbH-Y)71Vbo mice

increased CD8-positive, alpha-beta T cell number ( J:157757 )

• male mice, which undergo negative selection, exhibit an increase in CD8 single positive cells in the T3-70^hi cell compartment compared with Plcg1^tm1Gcrp/Plcg1⁺ Tg(CD4-cre)1Cwi Tg(TcraH-Y,TcrbH-Y)71Vbo mice

endocrine/exocrine glands

thymus hyperplasia ( J:157757 )

• in male mice, which undergo negative selection, compared with Plcg1^tm1Gcrp/Plcg1⁺ Tg(CD4-cre)1Cwi Tg(TcraH-Y,TcrbH-Y)71Vbo mice