Phenotypes associated with this allele

• Allele Symbol: Il4^tm1.1Wep
• Allele Name: targeted mutation 1.1, William E Paul
• Allele ID: MGI:2388164
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Il4^tm1.1Wep/Il4^tm1.1Wep	C.129P2-Il4^tm1.1Wep	MGI:3818432
hm2	Il4^tm1.1Wep/Il4^tm1.1Wep	involves: 129P2/OlaHsd * C57BL/6	MGI:3818339
ht3	Il4^tm1.1Wep/Il4^+	C.129P2-Il4^tm1.1Wep	MGI:3818433
cx4	Il4^tm1.1Wep/Il4^+ Stat6^tm1Gru/Stat6^tm1Gru	C.129-Stat6^tm1Gru Il4^tm1.1Wep	MGI:3818430
cx5	Il4^tm1.1Wep/Il4^tm1.1Wep Stat6^tm1Gru/Stat6^tm1Gru	C.129-Stat6^tm1Gru Il4^tm1.1Wep	MGI:3818431
cx6	Il13^tm1Anjm/Il13^+ Il4^tm1.1Wep/Il4^+	involves: 129P2/OlaHsd * BALB/c * C57BL/6	MGI:3818436

Genotype
MGI:3818432

hm1

• Allelic Composition: Il4^tm1.1Wep/Il4^tm1.1Wep
• Genetic Background: C.129P2-Il4^tm1.1Wep

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal T-helper 2 cell differentiation ( J:138827 )

• despite absent IgE and impaired eosinophil recruitment, in vivo Th2 cell differentiation in response to a parasitic nematode infection is normal with Th2 cell numbers peaking at days 9-12 after primary infection and days 5-7 after secondary infection

• these Th2 cells upregulate mRNA encoding for IL-13, IL-5, and GATA-3 proteins

• normal in vivo Th2 cell differentiation is also observed in the Th2 priming models of allergic airway using either keyhole limpet hemocyanin or OVA sensitization, IgD neutralization, and intradermal ear injection of dead nematodes

impaired eosinophil recruitment ( J:138827 )

• eosinophil recruitment to the airways is impaired during infection with a parasitic nematode compared to controls which has a sizable migration for the first two weeks after primary and secondary infections

decreased IgE level ( J:138827 )

• serum IgE is absent 15 days after parasitic nematode infection compared to the 400 ng/ml present in controls

• serum IgE is also absent when the allergic airway model or the IgD neutralization model is induced

hematopoietic system

abnormal T-helper 2 cell differentiation ( J:138827 )

• despite absent IgE and impaired eosinophil recruitment, in vivo Th2 cell differentiation in response to a parasitic nematode infection is normal with Th2 cell numbers peaking at days 9-12 after primary infection and days 5-7 after secondary infection

• these Th2 cells upregulate mRNA encoding for IL-13, IL-5, and GATA-3 proteins

• normal in vivo Th2 cell differentiation is also observed in the Th2 priming models of allergic airway using either keyhole limpet hemocyanin or OVA sensitization, IgD neutralization, and intradermal ear injection of dead nematodes

impaired eosinophil recruitment ( J:138827 )

• eosinophil recruitment to the airways is impaired during infection with a parasitic nematode compared to controls which has a sizable migration for the first two weeks after primary and secondary infections

decreased IgE level ( J:138827 )

• serum IgE is absent 15 days after parasitic nematode infection compared to the 400 ng/ml present in controls

• serum IgE is also absent when the allergic airway model or the IgD neutralization model is induced

Genotype
MGI:3818339

hm2

• Allelic Composition: Il4^tm1.1Wep/Il4^tm1.1Wep
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

decreased interleukin-4 secretion ( J:67964 )

• T cells do not secrete IL-4

Genotype
MGI:3818433

ht3

• Allelic Composition: Il4^tm1.1Wep/Il4⁺
• Genetic Background: C.129P2-Il4^tm1.1Wep

immune system

decreased IgE level ( J:138827 )

• serum IgE is half that of controls 15 days after parasitic nematode infection

• serum IgE is also significantly reduced when the allergic airway model or the IgD neutralization model is induced

hematopoietic system

decreased IgE level ( J:138827 )

• serum IgE is half that of controls 15 days after parasitic nematode infection

• serum IgE is also significantly reduced when the allergic airway model or the IgD neutralization model is induced

Genotype
MGI:3818430

cx4

• Allelic Composition: Il4^tm1.1Wep/Il4⁺; Stat6^tm1Gru/Stat6^tm1Gru
• Genetic Background: C.129-Stat6^tm1Gru Il4^tm1.1Wep

immune system

abnormal T-helper 2 cell differentiation ( J:138827 )

• CD4⁺ T cells fail to express IL-4 or GFP after in vitro activation under Th2 conditions and instead produce IFN-gamma (8%)

• these in vitro activated T cells also fail to upregulate mRNA encoding for IL-13, IL-5, IL-10, and GATA-3 proteins

• however, in vivo Th2 cell differentiation in response to a parasitic nematode infection is normal with Th2 cell numbers peaking at days 9-12 after primary infection and days 5-7 after secondary infection

• these Th2 cells upregulate mRNA encoding for IL-13, IL-5, and GATA-3 proteins

• normal in vivo Th2 cell differentiation is also observed in the Th2 priming models of allergic airway using either keyhole limpet hemocyanin or OVA sensitization, IgD neutralization, and intradermal ear injection of dead nematodes

impaired eosinophil recruitment ( J:138827 )

• eosinophil recruitment to the airways does not occur during infection with a parasitic nematode compared to controls which has a sizable migration for the first two weeks after primary and secondary infections

decreased IgE level ( J:138827 )

• serum IgE is absent 15 days after parasitic nematode infection compared to the 400 ng/ml present in controls

• serum IgE is also absent when the allergic airway model or the IgD neutralization model are induced

hematopoietic system

abnormal T-helper 2 cell differentiation ( J:138827 )

• CD4⁺ T cells fail to express IL-4 or GFP after in vitro activation under Th2 conditions and instead produce IFN-gamma (8%)

• these in vitro activated T cells also fail to upregulate mRNA encoding for IL-13, IL-5, IL-10, and GATA-3 proteins

• however, in vivo Th2 cell differentiation in response to a parasitic nematode infection is normal with Th2 cell numbers peaking at days 9-12 after primary infection and days 5-7 after secondary infection

• these Th2 cells upregulate mRNA encoding for IL-13, IL-5, and GATA-3 proteins

• normal in vivo Th2 cell differentiation is also observed in the Th2 priming models of allergic airway using either keyhole limpet hemocyanin or OVA sensitization, IgD neutralization, and intradermal ear injection of dead nematodes

impaired eosinophil recruitment ( J:138827 )

• eosinophil recruitment to the airways does not occur during infection with a parasitic nematode compared to controls which has a sizable migration for the first two weeks after primary and secondary infections

decreased IgE level ( J:138827 )

• serum IgE is absent 15 days after parasitic nematode infection compared to the 400 ng/ml present in controls

• serum IgE is also absent when the allergic airway model or the IgD neutralization model are induced

Genotype
MGI:3818431

cx5

• Allelic Composition: Il4^tm1.1Wep/Il4^tm1.1Wep; Stat6^tm1Gru/Stat6^tm1Gru
• Genetic Background: C.129-Stat6^tm1Gru Il4^tm1.1Wep

hematopoietic system

abnormal T-helper 2 cell differentiation ( J:138827 )

• CD4⁺ T cells fail to express IL-4 or GFP after in vitro activation under Th2 conditions

• these in vitro activated T cells also fail to upregulate mRNA encoding for IL-13, IL-5, IL-10, and GATA-3 proteins

• however, in vivo Th2 cell differentiation in response to a parasitic nematode infection is normal with Th2 cell numbers peaking at days 9-12 after primary infection and days 5-7 after secondary infection

• these Th2 cells upregulate mRNA encoding for IL-13, IL-5, and GATA-3 proteins

• normal in vivo Th2 cell differentiation is also observed in the Th2 priming models of allergic airway using either keyhole limpet hemocyanin or OVA sensitization, IgD neutralization, and intradermal ear injection of dead nematodes

impaired eosinophil recruitment ( J:138827 )

• eosinophil recruitment to the airways does not occur during infection with a parasitic nematode compared to controls which has a sizable migration for the first two weeks after primary and secondary infections

decreased IgE level ( J:138827 )

• serum IgE is absent 15 days after parasitic nematode infection compared to the 400 ng/ml present in controls

• serum IgE is also absent when the allergic airway model or the IgD neutralization model is induced

immune system

abnormal T-helper 2 cell differentiation ( J:138827 )

• CD4⁺ T cells fail to express IL-4 or GFP after in vitro activation under Th2 conditions

• these in vitro activated T cells also fail to upregulate mRNA encoding for IL-13, IL-5, IL-10, and GATA-3 proteins

• however, in vivo Th2 cell differentiation in response to a parasitic nematode infection is normal with Th2 cell numbers peaking at days 9-12 after primary infection and days 5-7 after secondary infection

• these Th2 cells upregulate mRNA encoding for IL-13, IL-5, and GATA-3 proteins

• normal in vivo Th2 cell differentiation is also observed in the Th2 priming models of allergic airway using either keyhole limpet hemocyanin or OVA sensitization, IgD neutralization, and intradermal ear injection of dead nematodes

impaired eosinophil recruitment ( J:138827 )

• eosinophil recruitment to the airways does not occur during infection with a parasitic nematode compared to controls which has a sizable migration for the first two weeks after primary and secondary infections

decreased IgE level ( J:138827 )

• serum IgE is absent 15 days after parasitic nematode infection compared to the 400 ng/ml present in controls

• serum IgE is also absent when the allergic airway model or the IgD neutralization model is induced

Genotype
MGI:3818436

cx6

• Allelic Composition: Il13^tm1Anjm/Il13⁺; Il4^tm1.1Wep/Il4⁺
• Genetic Background: involves: 129P2/OlaHsd * BALB/c * C57BL/6

immune system

decreased interleukin-4 secretion ( J:69099 )

• F1 crosses between mice homozygote for either allele creates offspring that has only one functional Il13 allele that is in cis with Il4^tm1.1Wep GFP reporter allele

• conversely, the one functional, wild-type Il4 allele is in cis with the null Il13^tm1Anjm allele

• CD4⁺ T cells from these mice primed in vitro under Th2 conditions have only 4.5% of T cells producing IL-4 compared to 14.8% of T cells producing IL-4 that are homozygous for wild-type Il13 and heterozygote for Il4^tm1.1Wep allele

• in contrast, GFP expression is similar in primed T cells from both sets of mice (5.9% and 6.8%)

• F1 crosses between 129 mice and Il4^tm1.1Wep homozygotes demonstrated the diminished IL-4 secretion was not the result of 129 polymorphisms in the Il4-Il13 genomic region