Analysis Tools|
Allele Symbol Allele Name Allele ID |
Hmox1tm1Poss targeted mutation 1, Kenneth D Poss MGI:2388322 |
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| Summary |
5 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• fewer than expected viable homozygous mice
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• decrease in the number of reticuloendothelial macrophages
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• decrease in the number of reticuloendothelial macrophages
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
Iron-loading of Hmox1tm1Poss/Hmox1tm1Poss tissues.
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• post-weaning survivors commonly die after 25 weeks of age; one homozygote lived up to 22 months
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• homozygotes exhibit low postnatal survival, with only 20% of the expected number of homozygotes obtained at 3 weeks; a similar survival % is observed in matings between homozygous and heterozygous mutant mice
• percentage of surviving homozygotes can be increased to 48% of those expected by in vitro fertilization techniques using gametes from homozygous and heterozygous animals
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• adult homozygotes exhibit enlarged spleens due to both extramedullary hematopoiesis and follicular hyperplasia
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• adult homozygotes display spleen follicular hyperplasia
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• adult homozygotes exhibit extramedullary hematopoiesis
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• at 20-24 weeks of age, homozygotes display normochromic and microcytic anemia which becomes severe by 40-55 weeks of age
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• at 10 (but not at 6) weeks of age, homozygotes start displaying significantly lower red blood cell counts relative to wild-type mice
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• at 10 weeks of age, homozygotes start displaying significantly reduced hematocrits relative to wild-type mice
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• at 10 (but not at 6) weeks of age, homozygotes start displaying significantly reduced blood hemoglobin concentrations relative to wild-type mice
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• at 10 weeks of age, homozygotes start displaying extremely reduced mean corpuscular volumes relative to wild-type mice
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• by 20 weeks of age, homozygotes exhibit variable erythrocyte size on blood smears
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• adult homozygotes exhibit high peripheral white blood cell counts
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• adult homozygotes display high splenic CD4+:CD8+ T-cell ratios with numerous activated CD4+ T cells
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• at day 3 after cisplatin administration, homozygotes exhibit significantly increased BUN values, indicating exacerbated renal dysfunction relative to similarly treated wild-type mice; no significant differences in body weight reduction are observed
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• despite iron deficiency, homozygotes exhibit progressively increasing serum ferritin levels up to ~50 weeks of age
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• by 20 weeks of age, homozygotes exhibit an increased total iron-binding capacity (high serum transferrin levels), resulting in a reduced iron saturation percentage and increased iron deposition relative to wild-type mice
• by ~50 weeks of age, all homozygotes exhibit pathological iron-loading (nonheme iron deposits) in renal proximal cortical tubules, liver Kupffer cells, hepatocytes, and hepatic vascular tissue (with variable severity) while the spleen remains unaffected
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• by 20 weeks of age, homozygotes display significantly reduced serum iron levels relative to wild-type mice
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• adult homozygotes exhibit enlarged spleens due to both extramedullary hematopoiesis and follicular hyperplasia
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• adult homozygotes display spleen follicular hyperplasia
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• adult homozygotes exhibit high peripheral white blood cell counts
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• adult homozygotes display high splenic CD4+:CD8+ T-cell ratios with numerous activated CD4+ T cells
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• adult homozygotes display high lymph node CD4+:CD8+ T-cell ratios with numerous activated CD4+ T cells
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• homozygotes develop a progressive chronic inflammatory disease
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• at ~50 weeks of age, homozygotes show hepatic inflammatory cell infiltrates, consisting of lymphocytes, plasma cells, neutrophils, and macrophages
• many infiltrates are periportal while others show a predilection for the portal venous tissue which often contains iron deposits
• hepatic vascular lesions invlove the proliferation of smooth muscle, the infiltration of neutrophils and lymphocytes into the vessel wall, and adherence of monocytes to the inner vessel wall
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• at ~50 weeks, homozygotes display glomerulonephritis, caused either by iron toxicity or by deposition of immune complexes
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• at ~50 weeks of age, homozygotes occasionally exhibit vascular and perivascular infiltrates in their lungs
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• at day 3 after cisplatin administration, homozygotes display a 6.6-fold increase in renal tubular apoptosis whereas wild-type mice show a 3-fold increase over saline-treated mice, respectively
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• at ~50 weeks, homozygotes display glomerulonephritis, caused either by iron toxicity or by deposition of immune complexes
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• by ~75 weeks, homozygotes display severely damaged glomeruli with membranous proliferation, lobularity, crescent formation, and sclerosis
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• by ~75 weeks, homozygotes display glomerular crescent formation
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• at day 3 after cisplatin-induced renal injury, homozygotes display more severe loss of brush border than wild-type mice
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• at day 3, cisplatin-treated homozygotes exhibit severe changes in acute renal injury, including tubular necrosis, degeneration, loss of brush border, red cell extravasation, tubular casts, and apoptotic bodies in the proximal and distal tubules; in contrast, wild-type mice display only mild changes with loss of brush border and red cell extravasation
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• at day 3 after cisplatin-induced renal injury, homozygotes display tubular casts
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• at day 3 after cisplatin-induced renal injury, homozygotes develop more severe renal failure than wild-type mice
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• at ~50 weeks of age, homozygotes show hepatic inflammatory cell infiltrates, consisting of lymphocytes, plasma cells, neutrophils, and macrophages
• many infiltrates are periportal while others show a predilection for the portal venous tissue which often contains iron deposits
• hepatic vascular lesions invlove the proliferation of smooth muscle, the infiltration of neutrophils and lymphocytes into the vessel wall, and adherence of monocytes to the inner vessel wall
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• at ~50 weeks of age, homozygotes display fibrosis within hepatic inflammatory cell infiltrates
• regenerative nodules indicative of hepatic injury are occasionally observed
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• as early as 25 weeks of age, most post-weaning survivors exhibit poor grooming
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• as early as 25 weeks of age, most post-weaning survivors appear less active than wild-type mice
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• homozygotes are slightly smaller than wild-type or heterozygous littermates from birth to early adulthood
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• between 20 and 40 weeks of age, most surviving homozygotes exhibit significant weight loss indicative of wasting
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• adult homozygotes exhibit enlarged spleens due to both extramedullary hematopoiesis and follicular hyperplasia
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• adult homozygotes display spleen follicular hyperplasia
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• at day 3 after cisplatin administration, homozygotes display a 6.6-fold increase in renal tubular apoptosis whereas wild-type mice show a 3-fold increase over saline-treated mice, respectively
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• as a result of iron deposition, livers from 20-24-wk-old homozygotes display increased oxidized proteins and lipid peroxidation values of 51% and 95% while kidneys show increases of 69% and 74% relative to heterozygous values, respectively
• notably, mutant brains, which have no iron deposition, show no evidence of enhanced oxidative damage
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• mature male homozygotes show a ~25% reduction in testicular size relative to similarly sized heterozygotes
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• as early as 25 weeks of age, most post-weaning survivors breed poorly
• mating pairs of homozygous mutant mice fail to yield viable litters
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• mature male homozygotes show a ~25% reduction in testicular size relative to similarly sized heterozygotes
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• at ~50 weeks of age, homozygotes occasionally exhibit vascular and perivascular infiltrates in their lungs
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Mouse Models of Human Disease |
DO ID | OMIM ID(s) | Ref(s) | |
| hemochromatosis | DOID:2352 |
OMIM:231100 OMIM:PS235200 |
J:79254 | |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• fewer than expected viable homozygous mice
|
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• decrease in the number of reticuloendothelial macrophages
|
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• decrease in the number of reticuloendothelial macrophages
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• decreased ratio of Hmox1 heterozygous, Slc48a1 homozygous offspring at birth indicating an almost 40% prenatal lethality
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• decrease in the total number of Ter-119+ cells in the bone marrow and spleen
• reduction in total Ter-119+ cells is increased compared to mice wild-type for Slc48a1
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• increase in immature erythroid precursor cells (population II+III) in the spleen
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• decrease in the number of reticuloendothelial macrophages
• reduction in splenic reticuloendothelial macrophages is increased compared to mice wild-type for Slc48a1
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• decrease in the number of reticuloendothelial macrophages
• reduction in splenic reticuloendothelial macrophages is increased compared to mice wild-type for Slc48a1
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• exposure to high levels of heme results in decreased numbers of bone marrow derived macrophages (BMDM) within 24 hours
• mice have reduced numbers of splenic red pulp macrophages and bone marrow macrophages
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• exposure to high levels of heme results in decreased numbers of bone marrow derived macrophages (BMDM) within 24 hours
• mice have reduced numbers of splenic red pulp macrophages and bone marrow macrophages
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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