Phenotypes associated with this allele

• Allele Symbol: Hmox2^tm1Poss
• Allele Name: targeted mutation 1, Kenneth D Poss
• Allele ID: MGI:2388325
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Hmox2^tm1Poss/Hmox2^tm1Poss	B6.129S2-Hmox2^tm1Poss	MGI:3612981
hm2	Hmox2^tm1Poss/Hmox2^tm1Poss	involves: 129S2/SvPas	MGI:4366929
hm3	Hmox2^tm1Poss/Hmox2^tm1Poss	involves: 129S2/SvPas * C57BL/6	MGI:3612980
cx4	Gucy1b2^tm3Mom/Gucy1b2^tm3Mom Hmox2^tm1Poss/Hmox2^tm1Poss	involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6J * FVB/N	MGI:6725099
cx5	Hmox2^tm1Poss/Hmox2^tm1Poss Nos1^tm1Plh/Nos1^tm1Plh	involves: 129S2/SvPas * 129S4/SvJae	MGI:4366930

Genotype
MGI:3612981

hm1

• Allelic Composition: Hmox2^tm1Poss/Hmox2^tm1Poss
• Genetic Background: B6.129S2-Hmox2^tm1Poss

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

respiratory system

abnormal respiration ( J:91075 )

• lung architecture and ventilation normal

• less responsive to acute hypoxia

abnormal vital capacity ( J:91075 )

• lower tidal volume and minute ventilataion

decreased pulmonary respiratory rate ( J:91075 )

• lower respiratory frequency

homeostasis/metabolism

abnormal circulating protein level ( J:91075 )

• reduced blood carboxyhemoglobin

hypoxia ( J:91075 )

• hypoxemia under ether anaesthesia

cardiovascular system

abnormal pulmonary vein morphology ( J:91075 )

• thickened walls of pulmonary veins due to hypertrophied pulmonary venous myocardium

• also overexpression of Hmox1

behavior/neurological

abnormal nociception after inflammation ( J:102990 )

• lower response to inflammatory pain as determined by formalin injection

• phase 1 licking time only 26% of controls and phase 2 time 40% of controls

Genotype
MGI:4366929

hm2

• Allelic Composition: Hmox2^tm1Poss/Hmox2^tm1Poss
• Genetic Background: involves: 129S2/SvPas

muscle

abnormal muscle physiology ( J:89581 )

• the resting membrane potential of jejunal smooth muscle cells is depolarized about 8 mV compared to wild type cells

• hyperpolarization and induction of an inhibitory junction potential in response to electrical field stimulation under nonadrenergic noncholinergic conditions in jejunal muscle strips are markedly reduced

• exposure to 10% CO gas partial restores nonadrenergic noncholinergic inhibitory transmission

abnormal muscle relaxation ( J:89581 )

• the decrease in muscle contractions in jejunal muscle strips in response to electrical field stimulation is markedly reduced

Genotype
MGI:3612980

hm3

• Allelic Composition: Hmox2^tm1Poss/Hmox2^tm1Poss
• Genetic Background: involves: 129S2/SvPas * C57BL/6

mortality/aging

increased susceptibility to induced morbidity/mortality ( J:46183 )

• increased susceptibility to hyperoxia (95% oxygen), died in 8.5 days as opposed to 13.4 days

nervous system

nervous system phenotype ( J:29523 )

N • brain morphology normal and basal synaptic functions all normal in the hippocampus, long term potentiation normal

abnormal nerve fiber response ( J:89562 )

• neuronal depolarization abnormalities result in failure of enteric smooth muscle relaxation

respiratory system

abnormal respiratory system physiology ( J:46183 )

• total glutathione in lungs increased over normal levels

• glutathione levels decrease 50% after 3 days of 95% hyperoxia

• 1.3 fold increase in lung protein carbonyls after 3 days of 95% hyperoxia

• increased Hmox1 levels after 3 days of 95% hyperoxia but not to the extent seen in controls

• higher levels of nitrotyrosine than in controls, indicating damage induced by NO

homeostasis/metabolism

abnormal iron level ( J:46183 )

• significantly increased levels of lung hemoproteins after exposure to hyperoxia

• heavy iron staining of lung endothelium after exposure to hyperoxia

• less lung ferritin protein protein after exposure to hyperoxia

digestive/alimentary system

abnormal intestinal transit time ( J:89562 )

• longer gastrointestinal transit times due to failure of enteric smooth muscle to relax normally

Genotype
MGI:6725099

cx4

• Allelic Composition: Gucy1b2^tm3Mom/Gucy1b2^tm3Mom; Hmox2^tm1Poss/Hmox2^tm1Poss
• Genetic Background: involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6J * FVB/N

homeostasis/metabolism

abnormal physiological response to hypoxia ( J:307157 )

• Type B olfactory sensory neurons of the main olfactory epithelium respond normally to NaHS but not reduced oxygen with HMOX2 absent from type B olfactory sensory neurons, homozygotes fail to respond to hypoxia by the usual avoidance behavior and increased stress-induced self-grooming that occurs in controls, but respond with normal avoidance and increased self-grooming in response to NaHS

Genotype
MGI:4366930

cx5

• Allelic Composition: Hmox2^tm1Poss/Hmox2^tm1Poss; Nos1^tm1Plh/Nos1^tm1Plh
• Genetic Background: involves: 129S2/SvPas * 129S4/SvJae

muscle

abnormal muscle physiology ( J:89581 )

• the resting membrane potential of jejunal smooth muscle cells is depolarized about 13 mV compared to wild type cells

• hyperpolarization and induction of an inhibitory junction potential in response to electrical field stimulation in jejunal muscle strips are markedly reduced

• unlike in wild-type controls electrical field stimulation under nonadrenergic noncholinergic conditions induces a depolarization of membrane potential in jejunal smooth muscle cells

abnormal muscle relaxation ( J:89581 )

• the decrease in muscle contractions in jejunal muscle strips in response to electrical field stimulation is markedly reduced