Phenotypes associated with this allele

• Allele Symbol: Tcra^tm1Cgn
• Allele Name: targeted mutation 1,University of Cologne
• Allele ID: MGI:2388328
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Tcra^tm1Cgn/Tcra^tm1Cgn Tg(Mx1-cre)1Cgn/0	involves: 129S7/SvEvBrd * C57BL/6 * CBA	MGI:3814172
cn2	Tcra^tm1Cgn/Tcra^tm1Mass Tg(Mx1-cre)1Cgn/0	involves: C57BL/6 * CBA	MGI:5532665
cn3	Tcra^tm1Cgn/Tcra^tm1Cgn Tg(Mx1-cre)1Cgn/0	involves: C57BL/6 * CBA	MGI:5532667

Genotype
MGI:3814172

cn1

• Allelic Composition: Tcra^tm1Cgn/Tcra^tm1Cgn; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal T cell morphology ( J:79298 )

• induction of cre expression at 6 weeks of age leads to loss of the T cell receptor complex (TCR)

• by 10 days after induction of cre, all T cells have lost normal expression of the TCR

• sensitive techniques reveal a very low level of TCR complex detectable on all T cells 10, 37 and 49 days after cre induction

abnormal CD4-positive, alpha beta T cell morphology ( J:79298 )

• the half-life decay of the CD4⁺ T cells after cre induction is 46 days compared to 78 days in controls

abnormal CD8-positive, alpha beta T cell morphology ( J:79298 )

• the half-life decay of the CD8⁺ T cells after cre induction is 16 days compared to 162 days in controls

abnormal memory T cell morphology ( J:79298 )

• after cre induction and loss of CD4 T cells, memory CD4 T cell numbers expand and fill the compartment

hematopoietic system

abnormal T cell morphology ( J:79298 )

• induction of cre expression at 6 weeks of age leads to loss of the T cell receptor complex (TCR)

• by 10 days after induction of cre, all T cells have lost normal expression of the TCR

• sensitive techniques reveal a very low level of TCR complex detectable on all T cells 10, 37 and 49 days after cre induction

abnormal CD4-positive, alpha beta T cell morphology ( J:79298 )

• the half-life decay of the CD4⁺ T cells after cre induction is 46 days compared to 78 days in controls

abnormal CD8-positive, alpha beta T cell morphology ( J:79298 )

• the half-life decay of the CD8⁺ T cells after cre induction is 16 days compared to 162 days in controls

abnormal memory T cell morphology ( J:79298 )

• after cre induction and loss of CD4 T cells, memory CD4 T cell numbers expand and fill the compartment

Genotype
MGI:5532665

cn2

• Allelic Composition: Tcra^tm1Cgn/Tcra^tm1Mass; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: C57BL/6 * CBA

immune system

increased T cell number ( J:201417 )

• uninduced mice exhibit an increase in tetramer+ T cells due to leaky cre expression

abnormal T cell physiology ( J:201417 )

• TCR-switched T cells treated with pIpC induce sterile inflammation in recipient mice possibly due to autoimmune activation

• however, recipient mice treated with galactosylceramide do not exhibit induction of NK T cell differentiation of TCR-switched tetramer+ T cells

hematopoietic system

increased T cell number ( J:201417 )

• uninduced mice exhibit an increase in tetramer+ T cells due to leaky cre expression

abnormal T cell physiology ( J:201417 )

• TCR-switched T cells treated with pIpC induce sterile inflammation in recipient mice possibly due to autoimmune activation

• however, recipient mice treated with galactosylceramide do not exhibit induction of NK T cell differentiation of TCR-switched tetramer+ T cells

Genotype
MGI:5532667

cn3

• Allelic Composition: Tcra^tm1Cgn/Tcra^tm1Cgn; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: C57BL/6 * CBA

immune system

immune system phenotype ( J:201417 )

N • pIpC-treated mice exhibit normal mature NK T cell maintenance, identity and secretion of cytokines