Phenotypes associated with this allele

• Allele Symbol: Il6st^tm1Ard
• Allele Name: targeted mutation 1, Ashley R Dunn
• Allele ID: MGI:2388476
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Il6st^tm1Ard/Il6st^tm1Ard	involves: 129S1/Sv * C57BL/6	MGI:3837031
cn2	Il6st^tm1Ard/Il6st^tm1Wme Tg(Alb1-cre)7Gsc/?	involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6 * FVB/N	MGI:3837204

Genotype
MGI:3837031

hm1

• Allelic Composition: Il6st^tm1Ard/Il6st^tm1Ard
• Genetic Background: involves: 129S1/Sv * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

increased susceptibility to xenobiotic induced morbidity/mortality ( J:79073 )

• remain moribund when exposure to 3.5% dextran sodium sulfate is ended

premature death ( J:79555 )

• 6 month life span for males

• 8 month life span for females

postnatal lethality, incomplete penetrance ( J:79555 )

• ratio of homozygotes normal at E18

• numbers deficient at weaning

growth/size/body

decreased body weight ( J:79555 )

• 20-30% lower body weight at birth

decreased body length ( J:79555 )

• 20-30% shorter trunk length at birth

reproductive system

failure of embryo implantation ( J:79555 )

♀ • failure of implantation, blastocysts will develop to term in wild-type recipient females

female infertility ( J:79555 )

♀ • females show no external signs of pregnancy regardless of male genotype

digestive/alimentary system

gastrointestinal hemorrhage ( J:79555 )

• fecal blood at 4 months of age

abnormal digestive system morphology ( J:79555 )

rectal prolapse ( J:79555 )

gastrointestinal ulcer ( J:79555 )

• caecal ulcers

• ulceration of the anorectal region

gastric ulcer ( J:79555 )

• ulceration of the gastric pylorus at 4 months of age

increased susceptibility to induced colitis ( J:79073 )

• severe erosion of the intestinal epithelium from exposure to 3.5% dextran sodium sulfate

• remain moribund when exposure is ended

cecum inflammation ( J:79555 )

• caecal inflammation

immune system

abnormal thrombopoiesis ( J:110742 )

• impaired maturation of megakaryocytes to produce platelets

• 40% reduction in the number of platelets produced per megakaryocyte

abnormal leukocyte cell number ( J:110742 )

• white blood cell count significantly higher than in controls

decreased immunoglobulin level ( J:79555 )

decreased IgA level ( J:79555 )

• basal IgA levels are reduced

• basal IgM is normal

• impaired ability to mount an intestinal IgA response after challenge

decreased IgG level ( J:79555 )

• impaired ability to mount an intestinal IgG response after challenge

increased inflammatory response ( J:79555 )

• nasolacrimal inflammation

increased susceptibility to induced colitis ( J:79073 )

• severe erosion of the intestinal epithelium from exposure to 3.5% dextran sodium sulfate

• remain moribund when exposure is ended

cecum inflammation ( J:79555 )

• caecal inflammation

conjunctivitis ( J:79555 )

increased susceptibility to parasitic infection ( J:79555 )

• tend to carry higher parasitic loads

hematopoietic system

abnormal common myeloid progenitor cell morphology ( J:110742 )

• colony forming cells elevated in the spleen but not in bone marrow

• elevated "CFU-S" hematopoietic progenitor cells

• hematopoietic progenitors elevated in the liver at E14 relative to controls

abnormal thrombopoiesis ( J:110742 )

• impaired maturation of megakaryocytes to produce platelets

• 40% reduction in the number of platelets produced per megakaryocyte

abnormal megakaryocyte morphology ( J:110742 )

decreased megakaryocyte cell number ( J:110742 )

• by 23% in bone marrow

increased megakaryocyte cell number ( J:110742 )

• numbers in spleen increased 2 fold relative to controls

thrombocytopenia ( J:110742 )

• platelet count reduced 30%

abnormal leukocyte cell number ( J:110742 )

• white blood cell count significantly higher than in controls

decreased immunoglobulin level ( J:79555 )

decreased IgA level ( J:79555 )

• basal IgA levels are reduced

• basal IgM is normal

• impaired ability to mount an intestinal IgA response after challenge

decreased IgG level ( J:79555 )

• impaired ability to mount an intestinal IgG response after challenge

skeleton

skeleton phenotype ( J:87612 )

N • trabecular bone volume is normal

abnormal femur morphology ( J:87612 )

decreased diameter of femur ( J:87612 )

• significantly reduced femoral width

short femur ( J:87612 )

abnormal joint morphology ( J:79555 )

joint swelling ( J:79555 )

• of weight bearing joints in 60% of adults

• observed by 7 days of age

• more severe in males than females

abnormal synovial joint capsule morphology ( J:79555 )

• cartilaginous nodules in joint capsules that replace menisci and support cartilage in knee joints

• erosion of articular surfaces in older mice

decreased joint mobility ( J:79555 )

• flexion abnormalities of hindlimbs

• reluctance to stand

• restricted flexion and extension of ankles and wrists

• stiffness of one or more limbs

homeostasis/metabolism

joint swelling ( J:79555 )

• of weight bearing joints in 60% of adults

• observed by 7 days of age

• more severe in males than females

increased susceptibility to xenobiotic induced morbidity/mortality ( J:79073 )

• remain moribund when exposure to 3.5% dextran sodium sulfate is ended

increased physiological sensitivity to xenobiotic ( J:79073 )

• severe erosion of the intestinal epithelium from exposure to 3.5% dextran sodium sulfate

cellular

increased cell proliferation ( J:79555 )

• proliferative response of fibroblasts in culture to Il6 is increased

cardiovascular system

gastrointestinal hemorrhage ( J:79555 )

• fecal blood at 4 months of age

vision/eye

conjunctivitis ( J:79555 )

endocrine/exocrine glands

abnormal involution of the mammary gland ( J:93986 )

♀ • delayed involution of transplanted mammary gland tissue

♀ • full involution does eventually occur

limbs/digits/tail

abnormal femur morphology ( J:87612 )

decreased diameter of femur ( J:87612 )

• significantly reduced femoral width

short femur ( J:87612 )

integument

abnormal involution of the mammary gland ( J:93986 )

♀ • delayed involution of transplanted mammary gland tissue

♀ • full involution does eventually occur

Genotype
MGI:3837204

cn2

• Allelic Composition: Il6st^tm1Ard/Il6st^tm1Wme; Tg(Alb1-cre)7Gsc/?
• Genetic Background: involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6 * FVB/N

mortality/aging

increased susceptibility to induced morbidity/mortality ( J:97327 )

• die within 8 hours of ConA treatment regardless of pretreatment with Il6

liver/biliary system

abnormal liver physiology ( J:97327 )

• liver damage due to ConA treatment regardless of pretreatment with Il6

abnormal liver regeneration ( J:135290 )

• maximal DNA synthesis in hepatocytes after partial hepatectomy and lipopolysaccharide treatment is reduced relative to controls

• 20% of cells as compared to 30% of cells