Phenotypes associated with this allele

• Allele Symbol: Myc^tm1Jlc
• Allele Name: targeted mutation 1, John L Cleveland
• Allele ID: MGI:2388702
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Myc^tm1Jlc/Myc^tm1Jlc	involves: 129S7/SvEvBrd	MGI:3812000
cx2	Apc^Min/Apc^+ Myc^tm1Jlc/Myc^+	involves: 129S7/SvEvBrd * C57BL/6 * C57BL/6J	MGI:3812011

Genotype
MGI:3812000

hm1

• Allelic Composition: Myc^tm1Jlc/Myc^tm1Jlc
• Genetic Background: involves: 129S7/SvEvBrd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

embryo

decreased embryo size ( J:79427 )

abnormal visceral yolk sac morphology ( J:79427 )

• at E9.5, the yolk sac is only 1 to 2 cell layers thick compared to 3 to 5 in wild-type mice

• at E9.5, PECAM1 (a marker for endothelial cells) staining is virtually absent from the yolk sac unlike in wild-type mice

abnormal embryonic erythropoiesis ( J:79427 )

• at E8.5 and E9.5, the number of primitive erythroid cells in the blood islands of the yolk sac and in the embryo proper are decreased compared to in wild-type mice

cardiovascular system

abnormal vascular development ( J:79427 )

• at E9.5, mice lack clearly defined vasculature including the dorsal aorta

• at E9.5, PECAM1 (a marker for endothelial cells) staining is absent unlike in wild-type mice

abnormal angiogenesis ( J:79427 )

abnormal dorsal aorta morphology ( J:79427 )

abnormal vasculogenesis ( J:79427 )

hematopoietic system

abnormal embryonic erythropoiesis ( J:79427 )

• at E8.5 and E9.5, the number of primitive erythroid cells in the blood islands of the yolk sac and in the embryo proper are decreased compared to in wild-type mice

decreased common myeloid progenitor cell number ( J:79427 )

• embryoid bodies derived from embryonic stem (ES) cells exhibit a modest reduction in their ability to form various myeloid cells compared to embryoid bodies derived from wild-type ES cells

• yolk sac cells fail to generate myeloid-like colonies when cultured unlike wild-type yolk sac cells

decreased erythroid progenitor cell number ( J:79427 )

• embryoid bodies derived from embryonic stem (ES) cells exhibit a modest reduction in their ability to form blast forming units-erythroid and a significant reduction in the number of colony forming units-erythroid compared to embryoid bodies derived from wild-type ES cells

• yolk sac cells fail to generate erythroid-like colonies when cultured and fewer colony forming units-erythroid compared to wild-type yolk sac cells

• however, treatment of embryoid bodies with VEGF restores their ability to form colony forming unit-erythroid

cellular

decreased cell proliferation ( J:79427 )

• at E8.5, proliferation of embryo and yolk sac cells is decreased compared to in wild-type mice

• embryonic stem (ES) cells exhibit a reduced proliferation and form fewer embryoid bodies than wild-type ES cells

neoplasm

decreased tumor incidence ( J:79427 )

• embryonic stem (ES) cells transplanted subcutaneously into Scid mice exhibit impaired tumorigenicity associated with decreased tumor vascularization compared to wild-type ES cells

growth/size/body

decreased embryo size ( J:79427 )

Genotype
MGI:3812011

cx2

• Allelic Composition: Apc^Min/Apc⁺; Myc^tm1Jlc/Myc⁺
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6 * C57BL/6J

mortality/aging

mortality/aging ( J:134087 )

N • mice exhibit a life span of 291 days compared to 169 days in Apc^Min heterozygotes

digestive/alimentary system

intestine polyps ( J:134087 )

• mice develop fewer and smaller polyps than in Apc^Min heterozygotes

abnormal digestive system physiology ( J:134087 )

• cell proliferation in polyps is decreased compared to in Apc^Min heterozygotes while levels of apoptosis are increased compared to in Apc^Min heterozygotes and wild-type mice

hematopoietic system

hematopoietic system phenotype ( J:134087 )

N • unlike in Apc^Min heterozygotes, hematocrit levels and spleen size are normal

abnormal hematopoietic system morphology/development ( J:134087 )

• expression of angiogenic factors is decreased compared to in Apc^Min heterozygotes