About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Gpx1tm1Kola
targeted mutation 1, Ismail Kola
MGI:2388705
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Gpx1tm1Kola/Gpx1tm1Kola B6.129S2(CF1)-Gpx1tm1Kola MGI:3606933
hm2
Gpx1tm1Kola/Gpx1tm1Kola involves: 129S2/SvPas * BALB/c * CF-1 MGI:2450520
hm3
Gpx1tm1Kola/Gpx1tm1Kola involves: 129S2/SvPas * CF-1 MGI:3606742


Genotype
MGI:3606933
hm1
Allelic
Composition
Gpx1tm1Kola/Gpx1tm1Kola
Genetic
Background
B6.129S2(CF1)-Gpx1tm1Kola
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gpx1tm1Kola mutation (2 available); any Gpx1 mutation (21 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
N
• upon infection with P. berghei ANKA or P. berghei K173, homozygotes show no significant differences in malaria parasite burdens relative to wild-type mice, suggesting normal intraerythrocytic parasite progression and malarial immunity




Genotype
MGI:2450520
hm2
Allelic
Composition
Gpx1tm1Kola/Gpx1tm1Kola
Genetic
Background
involves: 129S2/SvPas * BALB/c * CF-1
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gpx1tm1Kola mutation (2 available); any Gpx1 mutation (21 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• at 24 hrs after cold-induced brain injury, homozygotes exhibit an accelerated neuroinflammatory response, with a significant increase in Mac-1+ cells which rapidly declines by 96 hrs and reappears at 10 days post-injury, suggesting changes in response/activation/recruitment of macrophages and microglia to this type of injury

nervous system
• at 24 hrs after ischemia/reperfusion damage in the mid cerebral artery (MCA), homozygotes exhibit increased neuronal apoptosis, as shown by accelerated caspase-3 activation and increased TUNEL staining in post-ischemic mutant brains (J:71798)
• at 24 and 96 hrs after cold-induced brain injury, homozygotes display significantly increased neuronal cell death in regions immediately proximal to the infarct core (penumbra) (J:79183)
• upon MCA stroke surgery, homozygotes display increased susceptibility to ischemia/reperfusion injury relative to wild-type mice
• in response to ischemia/reperfusion damage in the MCA, homozygotes exhibit a a significantly increased infarct size (area in mm2) relative to wild-type mice
• in response to ischemia/reperfusion damage in the MCA, homozygotes show an increased neurological deficit score (1.97 vs 0.9) and higher mortality rates (19% vs 7%) relative to wild-type mice, with most mutants dying from severe stroke rather than surgical complications

cellular
• at 24 hrs after ischemia/reperfusion damage in the mid cerebral artery (MCA), homozygotes exhibit increased neuronal apoptosis, as shown by accelerated caspase-3 activation and increased TUNEL staining in post-ischemic mutant brains (J:71798)
• at 24 and 96 hrs after cold-induced brain injury, homozygotes display significantly increased neuronal cell death in regions immediately proximal to the infarct core (penumbra) (J:79183)
• in response to ischemia/reperfusion injury, homozygotes exhibit a significant increase in lipid hydroperoxide levels after 24 hrs of reperfusion relative to wild-type mice

homeostasis/metabolism
• after cold-induced brain injury, homozygotes exhibit no significant differences in infarct size or blood-brain barrier integrity relative to similarly injured wild-type mice
• however, at 24 and 96 hrs after cold-induced brain injury, homozygotes exhibit a significant increase in the number of TUNEL-positive neurons with apoptotic nuclear morphology
• upon MCA stroke surgery, homozygotes display increased susceptibility to ischemia/reperfusion injury relative to wild-type mice
• in response to ischemia/reperfusion damage in the MCA, homozygotes exhibit a a significantly increased infarct size (area in mm2) relative to wild-type mice




Genotype
MGI:3606742
hm3
Allelic
Composition
Gpx1tm1Kola/Gpx1tm1Kola
Genetic
Background
involves: 129S2/SvPas * CF-1
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gpx1tm1Kola mutation (2 available); any Gpx1 mutation (21 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
N
• homozygotes are obtained at the expected Mendelian ratios, and are fertile and healthy up to 12 months of age
• no histologic abnormalities are observed in brain, liver, kidney, lung, or heart at the age of 6 weeks

cellular
• in culture, mutant mouse embryonic fibroblasts (MEFs) display senescence-like changes, including a 3.5-fold increase in adherent cell surface area, increased Cip1 expression levels, and a 3-fold elevation in NF-kappaB activation relative to wild-type MEFs
• in culture, mutant MEFs exhibit significantly reduced [3H]-thymidine uptake, indicating reduced DNA synthesis and cellular growth relative to wild-type MEFs
• homozygotes exhibit increased susceptibility to paraquat toxicity in a dose-dependent fashion; at 30 mg/kg body weight, 100% of homozygotes die within 5 hrs whereas wild-type mice remain unaffected even up to 10 days after i.p. injection (J:49644)
• in culture, cortical neurons isolated from mutant mice show increased susceptibility to hydrogen peroxide-induced toxicity; at 65 M H2O2, 30% of mutant neurons die whereas all wild-type neurons remain viable (J:49644)
• in culture, mutant MEFs exhibit a significant dose-dependent susceptibility to oxidative stress-mediated cell death (J:87985)
• notably, in the annexin/PI assay, mutant MEFs show a small but significant increase in apoptosis in the absence of an added oxidant stress (J:87985)
• in culture, mutant MEFs exhibit a significant dose-dependent susceptibility to H2O2-mediated cell death
• upon treatment with high concentrations of H2O2, mutant MEFs exhibit significantly increased cellular debris, indicating higher susceptibility to necrotic cell death
• in culture, mutant MEFs exhibit significantly reduced cell population doubling rates relative to wild-type MEFs
• also, mutant MEFs show reduced responsiveness to the mitogenic properties of EGF and 10% FCS, indicating cell senescence





Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
10/29/2024
MGI 6.24
The Jackson Laboratory