All Phenotypes Mycn<tm1Psk> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Mycn^tm1Psk/Mycn⁺ Tg(Sftpc-cre)1Blh/0	involves: 129 * C57BL/6 * ICR	MGI:3575016
cn2	Mycn^tm1Psk/Mycn^tm1Psk Tg(Sftpc-cre)1Blh/0	involves: 129 * C57BL/6 * ICR	MGI:3575015
cn3	Mycn^tm1Psk/Mycn^tm1Psk Tg(Nes-cre)1Kln/0	involves: 129 * C57BL/6 * SJL	MGI:3836814

Allelic Composition	Mycn^tm1Psk/Mycn⁺ Tg(Sftpc-cre)1Blh/0
Genetic Background	involves: 129 * C57BL/6 * ICR

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mycn^tm1Psk mutation (2 available); any Mycn mutation (25 available)
Tg(Sftpc-cre)1Blh mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

decreased survivor rate ( J:97215 )

• ~50% of mutants develop normally into adulthood with no obvious lung defects

neonatal lethality, incomplete penetrance ( J:97215 )

• ~50% of mutants die at or shortly after birth with a severe lung phenotype

respiratory system

abnormal lung morphology ( J:97215 )

• at E18.5 the lungs of affected mutants are composed of numerous large fluid-filled sacs containing cellular debris lined by highly attenuated epithelial cells separated by a thin layer of mesoderm

• however, lung size, lobulation pattern, and trachea morphology are normal

abnormal lung development ( J:97215 )

• at E15.5-E16.5, a reduction in the proportion of proliferating cells in the endoderm is seen

• at E15.5, E16.5 and E18.5, abundant apoptotic cells are detected in the lumen, epithelium and mesenchyme, unlike in wild-type lungs

abnormal branching involved in lung morphogenesis ( J:97215 )

• at E14.5-E16.5 in affected mutants, branching is reduced with only a few expanded tubes separated by abundant mesoderm present

abnormal lung epithelium morphology ( J:97215 )

• at E14.5, E15.5 and E18.5, epithelial cell size is irregular, dead cells are seen in the lumen, and increased apoptosis is seen

decreased type I pneumocyte number ( J:97215 )

• at E18.5, attenuated presumptive type I cells are lining the large sacs

decreased type II pneumocyte number ( J:97215 )

• at E18.5, a paucity of type II cells is observed in the mutant lung

Allelic Composition	Mycn^tm1Psk/Mycn^tm1Psk Tg(Sftpc-cre)1Blh/0
Genetic Background	involves: 129 * C57BL/6 * ICR

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mycn^tm1Psk mutation (2 available); any Mycn mutation (25 available)
Tg(Sftpc-cre)1Blh mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

neonatal lethality, complete penetrance ( J:97215 )

• all mutants die at birth

respiratory system

abnormal lung morphology ( J:97215 )

• at E18.5 the lungs are composed of numerous large fluid-filled sacs containing cellular debris lined by highly attenuated epithelial cells separated by a thin layer of mesoderm

• however, lung size, lobulation pattern, and trachea morphology are normal

abnormal lung development ( J:97215 )

• at E15.5-E16.5, a reduction in the proportion of proliferating cells in the endoderm is seen (17% compared to 65% in wild-type at E15.5)

• at E15.5, E16.5 and E18.5, abundant apoptotic cells are detected in the lumen, epithelium and mesenchyme, unlike in wild-type lungs

abnormal branching involved in lung morphogenesis ( J:97215 )

• at E14.5-E16.5, branching is reduced with only a few expanded tubes separated by abundant mesoderm present

abnormal lung epithelium morphology ( J:97215 )

• at E14.5, E15.5 and E18.5, epithelial cell size is irregular, dead cells are seen in the lumen, and increased apoptosis is seen

abnormal pulmonary alveolus epithelial cell morphology ( J:97215 )

• expression of Aqp5 (a marker for type I cells) is prematurely increased at E16, suggesting premature differentiation of epithelial cells

decreased type I pneumocyte number ( J:97215 )

• at E18.5, attenuated presumptive type I cells are lining the large sacs

decreased type II pneumocyte number ( J:97215 )

• at E18.5, a paucity of type II cells is observed in the mutant lung

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

growth/size/body

microcephaly ( J:79489 )

• decrease in head size can be detected at E12.5

postnatal growth retardation ( J:79489 )

• moderate growth retardation

nervous system

nervous system phenotype ( J:79489 )

N	• neuronal precursor cell apoptosis is similar to controls

abnormal neuron differentiation ( J:79489 )

• progenitor cells display smaller nuclei

premature neuronal precursor differentiation ( J:79489 )

• premature differentiation is seen both in vivo and in vitro

abnormal neuronal precursor proliferation ( J:79489 )

• striking reduction in proliferation in the central nervous system in general at E13

• only a very small fraction of cells in the interior of the cerebellum primordium are proliferating at E13

• significant decrease in the number of proliferating cells in the ventricular zone of the telencephalon at E13

abnormal embryonic neuroepithelium morphology ( J:79489 )

• the cerebellar neuroepithelium appears thinner, displays breaks, and has a deficiency in progenitor cells

decreased embryonic neuroepithelium thickness ( J:79489 )

• the cerebellar neuroepithelium appears thinner

abnormal cortical ventricular zone morphology ( J:79489 )

• many regions of the VZ are noticeably reduced in thickness

• at E17.5 in the lateral VZ neuroprogenitor cells are more rounded and more densely packed

decreased brain size ( J:79489 )

• at 8 - 16 weeks of age mice display profound microencephaly

• decrease in brain size can be detected at E12.5

• total brain mass relative to total body weight is reduced 2 fold compared to controls

abnormal cerebral cortex morphology ( J:79489 )

• the developing cerebral cortex is particularly small

abnormal cerebellum morphology ( J:79489 )

• cerebellum appears disorganized

• at P20 in the rostral region all 3 layers are severely affected

abnormal cerebellum development ( J:79489 )

• profound reduction in the progenitor cell pool in both the external granule cell layer and in the neuroepithelium

• at E12.5 a significant reduction in neuroprogenitor cell numbers is seen in the cerebellar neuroepithelium and rhombic lip and the interior differentiating zone is reduced in size

• at E17.5 a subset of rhombic lip neuroprogenitor cells are more rounded and more densely packed

abnormal cerebellar foliation ( J:79489 )

• severe defects in foliation

abnormal cerebellum external granule cell layer morphology ( J:79489 )

• profound reduction in the progenitor cell pool

• decrease is more pronounced in the rostral region

• the most rostral portion of the EGL is absent

abnormal cerebellar Purkinje cell layer ( J:79489 )

• disrupted

decreased Purkinje cell number ( J:79489 )

ectopic Purkinje cell ( J:79489 )

abnormal cerebellar granule layer morphology ( J:79489 )

• granule cell density is reduced 3- to 6-fold

• at P20 total granule cell numbers are reduced about 30-fold

abnormal cerebellar molecular layer ( J:79489 )

• fails to expand resulting in decreased area and decreased cell number

small cerebellum ( J:79489 )

• the developing cerebellum is particularly small

• at P20 the absolute weight and percent of total brain weight of the cerebellum is reduced by 4- to 6-fold compared to controls

decreased neuronal precursor cell number ( J:79489 )

• in the cerebellar neuroepithelium, rhombic lip, and external granule cell layer at E12.5 and E17.5

vision/eye

microphthalmia ( J:79489 )

embryo