Phenotypes associated with this allele

• Allele Symbol: Foxd3^tm1Lby
• Allele Name: targeted mutation 1, Patricia A Labosky
• Allele ID: MGI:2388719
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Foxd3^tm1Lby/Foxd3^tm1Lby	either: (involves: 129S6/SvEvTac) or (involves: 129S6/SvEvTac * Black Swiss) or (involves: 129S6/SvEvTac * C57BL/6) or (involves: 129S6/SvEvTac * CD-1)	MGI:3662915
hm2	Foxd3^tm1Lby/Foxd3^tm1Lby	involves: 129S6/SvEvTac * CD-1	MGI:3662919

Genotype
MGI:3662915

hm1

• Allelic Composition: Foxd3^tm1Lby/Foxd3^tm1Lby
• Genetic Background: either: (involves: 129S6/SvEvTac) or (involves: 129S6/SvEvTac * Black Swiss) or (involves: 129S6/SvEvTac * C57BL/6) or (involves: 129S6/SvEvTac * CD-1)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality, complete penetrance ( J:79491 )

embryo

abnormal anterior visceral endoderm cell migration ( J:79491 )

• at E6.5 the anterior visceral endoderm remains at the distal tip of the embryo unlike in wild-type mice where this tissue has moved anteriorly

abnormal developmental patterning ( J:79491 )

failure to gastrulate ( J:79491 )

• gastrulation does not occur

abnormal proximal-distal axis patterning ( J:79491 )

• by E7.5 the entire distal end of the embryo is severely truncated

• expression of extraembryonic cell markers is expanded distally

abnormal embryonic epiblast morphology ( J:79491 )

• transplanted E6.5 epiblasts fail to generate teratocarcinomas suggesting loss of the pluripotent cells

decreased embryonic epiblast cell proliferation ( J:79491 )

• decreased cell proliferation at E6.5

small embryonic epiblast ( J:79491 )

• small at E6.5

absent mesoderm ( J:79491 )

• at E6.5 expression analysis indicates that mesodermal cells are not formed

failure of primitive streak formation ( J:79491 )

abnormal extraembryonic endoderm formation ( J:79491 )

• at E6.5, finger-like protrusions of cells resembling extraembryonic endoderm are found at the distal tip of the embryo

cellular

abnormal anterior visceral endoderm cell migration ( J:79491 )

• at E6.5 the anterior visceral endoderm remains at the distal tip of the embryo unlike in wild-type mice where this tissue has moved anteriorly

abnormal cell proliferation ( J:79491 )

• unable to generate homozygous ES cells either from blastocysts or by retargeting heterozygous ES cells

• transplanted E6.5 epiblasts fail to generate teratocarcinomas suggesting loss of the pluripotent cells

decreased embryonic epiblast cell proliferation ( J:79491 )

• decreased cell proliferation at E6.5

Genotype
MGI:3662919

hm2

• Allelic Composition: Foxd3^tm1Lby/Foxd3^tm1Lby
• Genetic Background: involves: 129S6/SvEvTac * CD-1

embryo

abnormal placenta labyrinth morphology ( J:101652 )

• labyrinthine precursors are absent

abnormal trophoblast layer morphology ( J:101652 )

• giant cells are produced but the spongiotrophoblast lineage is absent

• marker analysis indicates that the fate of trophoblast precursor cells is shifted towards production of giant cells and away from development of spongiotrophoblast and labyrinthine lineages

• undifferentiated trophoectodermal cells are present at E8.0 but absent by E9.0 indicating a failure to maintain the multipotent progenitor cell population

• unable to derive trophoblast stem cell lines from E3.5 embryos or E6.5 extraembryonic ectoderm

absent spongiotrophoblast ( J:101652 )

• the spongiotrophoblast lineage is absent