About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Zap70tm1.1Mmal
targeted mutation 1.1, Marie Malissen
MGI:2388913
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Zap70tm1.1Mmal/Zap70tm1.1Mmal B6.129P2-Zap70tm1.1Mmal MGI:3790314
cx2
 		Zap70tm1.1Mmal/Zap70tm1.1Mmal
Tg(TcrLCMV)327Sdz/0 		involves: 129P2/OlaHsd * BALB/cJ * C57BL/6 * DBA/2 MGI:3790319
cx3
 		Zap70tm1.1Mmal/Zap70tm1.1Mmal
Tg(TcraH-Y,TcrbH-Y)71Vbo/0 		involves: 129P2/OlaHsd * BALB/cJ * C57BL/6J * DBA/2J MGI:3790316


Genotype
MGI:3790314
hm1
Allelic
Composition		 Zap70tm1.1Mmal/Zap70tm1.1Mmal
Genetic
Background		 B6.129P2-Zap70tm1.1Mmal
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Zap70tm1.1Mmal mutation (0 available); any Zap70 mutation (55 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:71099 )
N
• mice show no abnormal immune organ cellularity or T lymphocyte representation




Genotype
MGI:3790319
cx2
Allelic
Composition		 Zap70tm1.1Mmal/Zap70tm1.1Mmal
Tg(TcrLCMV)327Sdz/0
Genetic
Background		 involves: 129P2/OlaHsd * BALB/cJ * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(TcrLCMV)327Sdz mutation (8 available)
Zap70tm1.1Mmal mutation (0 available); any Zap70 mutation (55 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:71099 )
N
• generation of CD8+/V alpha2high thymocytes and lymph node T cells is normal
• double negative and double positive cells from thymi of female mutants and wild-type mice have similar levels of CD5 expression
abnormal T cell proliferation ( J:71099 )
• when challenged with subnanomolar concentrations of p33 peptide in vitro, T cells proliferate more than wild-type Tg(TcrLCMV)327Sdz T cells
abnormal cytokine secretion ( J:71099 )
• T cells produce 2-3-fold more IL-2 in response to graded p33 concentrations in vitro compared to stimulated wild-type Tg(TcrLCMV)327Sdz or Zap70tm2.1Mmal/ Tg(TcrLCMV)327Sdz T cells
• this is not observed at a single-cell level of cytokine production, indicating that increased IL-2 is result of greater T cell numbers

hematopoietic system
abnormal T cell proliferation ( J:71099 )
• when challenged with subnanomolar concentrations of p33 peptide in vitro, T cells proliferate more than wild-type Tg(TcrLCMV)327Sdz T cells

cellular
abnormal T cell proliferation ( J:71099 )
• when challenged with subnanomolar concentrations of p33 peptide in vitro, T cells proliferate more than wild-type Tg(TcrLCMV)327Sdz T cells




Genotype
MGI:3790316
cx3
Allelic
Composition		 Zap70tm1.1Mmal/Zap70tm1.1Mmal
Tg(TcraH-Y,TcrbH-Y)71Vbo/0
Genetic
Background		 involves: 129P2/OlaHsd * BALB/cJ * C57BL/6J * DBA/2J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(TcraH-Y,TcrbH-Y)71Vbo mutation (4 available)
Zap70tm1.1Mmal mutation (0 available); any Zap70 mutation (55 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:71099 )
N
• numbers of CD8+/T3.70high thymocytes and lymph node T cells are normal, indicating that the Zap70 mutation has no effect on positive selection of T cells
• double negative and double positive cells from thymi of female mutants and wild-type mice have similar levels of CD5 expression




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
12/10/2024
MGI 6.24 		The Jackson Laboratory