Analysis Tools
adipose tissue
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• at 20 weeks, increased body weight was correlated with a 2-fold increase in the epididymal and lumbar fat pad deposition; however, no differences were detected in the amount of brown adipose tissue fat pad or in blood glucose levels
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behavior/neurological
| N |
• intracerebroventricular (i.c.v.) injection of neurotensin induced a similar analgesic effect on PBQ-induced writhing in homozygous null and wild-type mice
• homozygous null mice displayed no significant differences in spontaneous locomotion relative to wild-type; however, in contrast to control littermates, mutant mice failed to respond to neurotensin-induced (400 ng, via i.c.v.) hypolocomotion
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polyphagia
(
J:79712
)
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• at 11 weeks, increased body weight in homozygous null males was correlated with a 10% increase in spontaneous food consumption relative to wild-type males; however, no significant differences were observed in water uptake, leptin, blood glucose, plasma insulin levels or in adipose tissue composition
• no differences were observed between wild-type and homozygous null mice in feeding behavior following 18 hours of food deprivation
• in wild-type mice, i.c.v. administration of 10 ng of neurotensin fully inhibited feeding for an hour, with a slow recovery in appetite; in contrast, neurotensin had no effect on the feeding behavior of mutant mice at either low or high neurotensin concentration
• i.c.v. administration of the orexigenic neuropeptide Y stimulated a similar increase in food consumption in wild-type and mutant male mice; also, the pattern of PYY binding was identical in both strains
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growth/size/body
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• both male and female homozygous null mice were viable, fertile, and displayed no obvious physical abnormalities
• starting at 10 weeks of age, homozygous null males became significantly heavier than wild-type males: a difference of 15% was noted at approximately 15 weeks persisting up to 45 weeks
• at 8 weeks, homozygous null females also displayed a small difference in body weight relative to wild-type; this difference persisted up to at least 12 weeks
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homeostasis/metabolism
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• untreated 11-week-old homozygous null mice were hyperthermic compared with wild-type littermates
• notably, i.c.v. administration of 200 ng of neurotensin to wild-type mice caused a drop in rectal temperature of 3.2 C; in contrast, i.c.v. injection of up to 400 ng neurotensin in homozygous null mice had no significant effect on rectal temperature
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