Phenotypes associated with this allele

• Allele Symbol: Tg(KRT5-Tert)8043Blas
• Allele Name: transgene insertion 8043, Maria A Blasco
• Allele ID: MGI:2429416
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Terc^tm1Rdp/Terc^tm1Rdp Tg(KRT5-Tert)8043Blas/0	involves: 129/Sv * C57BL/6 * C57BL/6J * DBA/2 * SJL	MGI:3700845
cx2	Tg(KRT5-Terf2)PMBlas/Y Tg(KRT5-Tert)8043Blas/0	involves: C57BL/6 * CBA * DBA/2	MGI:6258420
tg3	Tg(KRT5-Tert)8043Blas/0	involves: C57BL/6 * DBA/2	MGI:3700914

Genotype
MGI:3700845

cx1

• Allelic Composition: Terc^tm1Rdp/Terc^tm1Rdp; Tg(KRT5-Tert)8043Blas/0
• Genetic Background: involves: 129/Sv * C57BL/6 * C57BL/6J * DBA/2 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

abnormal chromosome morphology ( J:96945 )

• mutants exhibit an increase in the frequency of chromosome aberrations in keratinocytes, in particular, of end-to-end fusions, breaks, and telomere associations

decreased telomere length ( J:96945 )

• keratinocytes exhibit a significant decrease in average telomere length compared with wild-type cells, similar to that seen in mutant Terc homozygotes

homeostasis/metabolism

decreased incidence of tumors by chemical induction ( J:96945 )

• mutants are less susceptible to DMBA + TPA induced skin tumorigenesis, with only 40% developing papillomas compared to 80%, 72% and 86% of wild-type, Tg(KRT5-Tert)8043Blas mutants, and Terc homozygous mutants, respectively

• the number of papillomas per mouse at week 15 is reduced compared to the above controls and papillomas never progress to lesions larger than 3 mm

delayed wound healing ( J:96945 )

• mutants exhibit a delayed rate of wound healing in skin compared to Tg(KRT5-Tert)8043Blas mutants

neoplasm

decreased incidence of tumors by chemical induction ( J:96945 )

• mutants are less susceptible to DMBA + TPA induced skin tumorigenesis, with only 40% developing papillomas compared to 80%, 72% and 86% of wild-type, Tg(KRT5-Tert)8043Blas mutants, and Terc homozygous mutants, respectively

• the number of papillomas per mouse at week 15 is reduced compared to the above controls and papillomas never progress to lesions larger than 3 mm

Genotype
MGI:6258420

cx2

• Allelic Composition: Tg(KRT5-Terf2)PMBlas/Y; Tg(KRT5-Tert)8043Blas/0
• Genetic Background: involves: C57BL/6 * CBA * DBA/2

pigmentation

increased skin pigmentation ( J:102653 )

• double mutant mice exhibit skin hyperpigmentation in response to light, similar to single Tg(KRT5-Terf2)PMBlas mutant mice

integument

increased skin pigmentation ( J:102653 )

• double mutant mice exhibit skin hyperpigmentation in response to light, similar to single Tg(KRT5-Terf2)PMBlas mutant mice

cellular

decreased telomere length ( J:102653 )

• double mutant mice exhibit short telomeres in tail skin, similar to single Tg(KRT5-Terf2)PMBlas mutant mice, indicating that critically short telomeres are not rescued by telomerase overexpression

Genotype
MGI:3700914

tg3

• Allelic Composition: Tg(KRT5-Tert)8043Blas/0
• Genetic Background: involves: C57BL/6 * DBA/2

homeostasis/metabolism

increased incidence of tumors by chemical induction ( J:69757 , J:96945 )

• epidermis is more susceptible to the development of skin tumors upon chemical carcinogenesis (DMBA + TPA), with an increase in the number of papillomas and an increase in mortality (J:69757)

• mutant mice are more susceptible to skin tumorigenesis induced by DMBA + TPA, developing about 1.5-fold more papillomas per mouse than wild-type at week 15 and papillomas progress to bigger lesions (J:96945)

enhanced wound healing ( J:69757 , J:96945 )

• epidermis shows an increase in wound healing rate compared to wild-type (J:69757)

neoplasm

increased incidence of tumors by chemical induction ( J:69757 , J:96945 )

• epidermis is more susceptible to the development of skin tumors upon chemical carcinogenesis (DMBA + TPA), with an increase in the number of papillomas and an increase in mortality (J:69757)

• mutant mice are more susceptible to skin tumorigenesis induced by DMBA + TPA, developing about 1.5-fold more papillomas per mouse than wild-type at week 15 and papillomas progress to bigger lesions (J:96945)

cellular

cellular phenotype ( J:69757 )

N • mutants exhibit normal telomere length

integument

skin hyperplasia ( J:69757 , J:96945 )

• TPA-treated skin shows a high number of skin keratinocyte layers (skin hyperplasia) that are not seen in controls, indicating a more sustained proliferative response of skin to TPA treatment (J:69757)