Phenotypes associated with this allele

• Allele Symbol: Pou4f2^tm2Whk
• Allele Name: targeted mutation 2, William H Klein
• Allele ID: MGI:2429634
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Pou4f2^tm1(Pou4f1)Gan/Pou4f2^tm2Whk	involves: 129S7/SvEvBrd * C57BL/6J	MGI:3574999
ht2	Pou4f2^tm1(ALPP)Whk/Pou4f2^tm2Whk	Not Specified	MGI:3575000
cn3	Isl1^tm1Gan/Isl1^tm2Gan Pou4f2^tm1(ALPP)Whk/Pou4f2^tm2Whk Tg(Six3-cre)69Frty/?	involves: 129S6/SvEvTac * C57BL/6 * DBA/2	MGI:3797797
cn4	Isl1^tm2Gan/Isl1^tm2Gan Pou4f2^tm1(ALPP)Whk/Pou4f2^tm2Whk Tg(Six3-cre)69Frty/?	involves: 129S6/SvEvTac * C57BL/6 * DBA/2	MGI:3797796

Genotype
MGI:3574999

ht1

• Allelic Composition: Pou4f2^{tm1(Pou4f1)Gan}/Pou4f2^tm2Whk
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

vision/eye

vision/eye phenotype ( J:96926 )

N • normal numbers of retinal ganglion cells and normal optic nerve morphology are seen

Genotype
MGI:3575000

ht2

• Allelic Composition: Pou4f2^tm1(ALPP)Whk/Pou4f2^tm2Whk
• Genetic Background: Not Specified

vision/eye

increased retina apoptosis ( J:96926 )

• at E15.5 retinal ganglion cell apoptosis is seen and at E17.5 loss of these cells is accelerated and propagated from the central to the peripheral retina

retina ganglion cell degeneration ( J:96926 )

• a loss of 70% of the retinal ganglion cells in the retinal ganglion cell layer is seen at 6 weeks of age

abnormal optic nerve morphology ( J:96926 )

• fewer and less fasciculated axon bundles projecting into the optic disc

• the cross-sectional area of the optic nerve is reduced

• an increased proportion of the axons project into the ipsilateral optic track or into the optic nerve of the opposite eye

abnormal optic nerve innervation ( J:134978 )

• retinal ganglion axons fail to reach the midline at E13.5

• At E15.5, the axons in the optic nerve are less fasciculated and appear to be grouped into two bundles

• in addition, a substantial portion of retinal ganglion cells fail to send out axons or their axons do not exit the optic cup

nervous system

retina ganglion cell degeneration ( J:96926 )

• a loss of 70% of the retinal ganglion cells in the retinal ganglion cell layer is seen at 6 weeks of age

abnormal optic nerve morphology ( J:96926 )

• fewer and less fasciculated axon bundles projecting into the optic disc

• the cross-sectional area of the optic nerve is reduced

• an increased proportion of the axons project into the ipsilateral optic track or into the optic nerve of the opposite eye

abnormal optic nerve innervation ( J:134978 )

• retinal ganglion axons fail to reach the midline at E13.5

• At E15.5, the axons in the optic nerve are less fasciculated and appear to be grouped into two bundles

• in addition, a substantial portion of retinal ganglion cells fail to send out axons or their axons do not exit the optic cup

cellular

increased retina apoptosis ( J:96926 )

• at E15.5 retinal ganglion cell apoptosis is seen and at E17.5 loss of these cells is accelerated and propagated from the central to the peripheral retina

Genotype
MGI:3797797

cn3

• Allelic Composition: Isl1^tm1Gan/Isl1^tm2Gan; Pou4f2^tm1(ALPP)Whk/Pou4f2^tm2Whk; Tg(Six3-cre)69Frty/?
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * DBA/2

vision/eye

retina ganglion cell degeneration ( J:134978 )

• retinal ganglion cells expressing Pou4f1 are severely reduced in adult retina

• adult mice only have about 5% the number of retinal ganglion cells as do the controls

• genesis of RGCs are not affected as they are present in E13.5 embryos

optic nerve hypoplasia ( J:134978 )

• the optic nerve is barely detectable in adults with only a very limited number of axon bundles present

nervous system

retina ganglion cell degeneration ( J:134978 )

• retinal ganglion cells expressing Pou4f1 are severely reduced in adult retina

• adult mice only have about 5% the number of retinal ganglion cells as do the controls

• genesis of RGCs are not affected as they are present in E13.5 embryos

optic nerve hypoplasia ( J:134978 )

• the optic nerve is barely detectable in adults with only a very limited number of axon bundles present

Genotype
MGI:3797796

cn4

• Allelic Composition: Isl1^tm2Gan/Isl1^tm2Gan; Pou4f2^tm1(ALPP)Whk/Pou4f2^tm2Whk; Tg(Six3-cre)69Frty/?
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * DBA/2

vision/eye

retina ganglion cell degeneration ( J:134978 )

• retinal ganglion cells expressing Pou4f1 are severely reduced in adult retina

• adult mice only have about 5% the number of retinal ganglion cells as do the controls

• genesis of RGCs are not affected as they are present in E13.5 embryos

optic nerve hypoplasia ( J:134978 )

• the optic nerve is barely detectable in adults with only a very limited number of axon bundles present

nervous system

retina ganglion cell degeneration ( J:134978 )

• retinal ganglion cells expressing Pou4f1 are severely reduced in adult retina

• adult mice only have about 5% the number of retinal ganglion cells as do the controls

• genesis of RGCs are not affected as they are present in E13.5 embryos

optic nerve hypoplasia ( J:134978 )

• the optic nerve is barely detectable in adults with only a very limited number of axon bundles present