All Phenotypes Prox1<+> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Prox1^tm1Gco/Prox1⁺	involves: 129S1/Sv * 129X1/SvJ	MGI:2669230
ht2	Prox1^tm1Gco/Prox1⁺	involves: 129S1/Sv * 129X1/SvJ * NMRI	MGI:2669229
ht3	Prox1^tm4.1Gco/Prox1⁺	involves: 129S1/Sv * C57BL/10 * CBA/Ca	MGI:4441391
ht4	Prox1^{tm5(GFP/cre)Gco}/Prox1⁺	involves: 129S1/Sv * NMRI	MGI:4441389
cn5	Gt(ROSA)26Sor^tm1Sor/Gt(ROSA)26Sor⁺ Nr2f2^tm2.1Tsa/Nr2f2^tm2.1Tsa Prox1^{tm3(cre/ERT2)Gco}/Prox1⁺	involves: 129S1/Sv * 129S4/SvJaeSor * 129S7/SvEvBrd * C57BL/6	MGI:4441394
cn6	Prox1^tm2Gco/Prox1⁺ Tg(Tek-cre)1Ywa/0	involves: 129S1/Sv * C57BL/6 * NMRI * SJL	MGI:3611343

Allelic Composition	Prox1^tm1Gco/Prox1⁺
Genetic Background	involves: 129S1/Sv * 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prox1^tm1Gco mutation (0 available); any Prox1 mutation (43 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

postnatal lethality, complete penetrance ( J:53431 )

• Background Sensitivity: all mice die within 3 days of birth unlike mice crossed to an NMRI background where 1 of 30 survive

cardiovascular system

abnormal lymph circulation ( J:57646 )

• the intestines are filled with a white fluid (probably chyle) a few hours before death

immune system

abnormal lymph circulation ( J:57646 )

• the intestines are filled with a white fluid (probably chyle) a few hours before death

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

postnatal lethality, incomplete penetrance ( J:53431 , J:102652 )

• Background Sensitivity: unlike on other backgrounds, 1 of 30 heterozygotes survive (J:53431)

respiratory system

chylothorax ( J:102652 )

• heterozygotes that die within a few days of birth have milky chylous ascites in the peritoneal cavity and/or thoracic cavity

respiratory distress ( J:102652 )

• heterozygotes that die without suckling are in respiratory distress

digestive/alimentary system

abnormal intestine morphology ( J:102652 )

• accumulation of lipid is seen in the intestine walls of heterozygotes that die postnatally

immune system

abnormal lymph circulation ( J:57646 , J:102652 )

• the intestines are filled with a white fluid (probably chyle) a few hours before death (J:57646)

• leakage of chyle from the mesenteric vessels (J:102652)

• dye injected into the dermal lymph vessels surrounding the popliteal and inguinal lymph nodes abnormally accumulates in the cutaneous lymphatics between the injection site and the draining lymph nodes (J:102652)

• dye injected into the foot pad accumulates in multiple hypoplastic and tortuous lymphatic vessels in the thoracic cavity rather than in the thoracic duct (J:102652)

increased macrophage cell number ( J:102652 )

• an increase number of Mac-2+ macrophages is seen along with liver lipid accumulation

abnormal lymphatic vessel morphology ( J:102652 )

• lymph vessels are mispatterned and dilated, with those in the intestine and mesentery most severely affected

• vessels sprout from the mesenteric lymphatics and invade the mesothelial membrane which does not normally contain lymph vessels

• some endothelial cells lining intestinal lymph ducts are discontinuous and ruptured

increased inflammatory response ( J:102652 )

• inflammation of the mesothelial membrane in obese adults

homeostasis/metabolism

increased circulating glucagon level ( J:102652 )

• after the onset of obesity serum glucagon is increased

increased circulating insulin level ( J:102652 )

• after the onset of obesity serum insulin is increased; however serum glucose levels are similar to wild-type

increased circulating leptin level ( J:102652 )

• serum leptin is increased after the onset of obesity but not before

increased circulating alanine transaminase level ( J:102652 )

• the serum ratio of alanine aminotransferase to aspartate aminotransferase is elevated in obese mice

cyanosis ( J:102652 )

• heterozygotes that die without suckling appear cyanotic

edema ( J:57646 , J:102652 )

• severe edema develops around E14.5 (J:57646)

• at E14.5 and at E16.5 all heterozygous embryos have edema; however edema is resolved before birth (J:102652)

• some heterozygotes in respiratory distress had clear fluid accumulation in the thoracic cavity (J:102652)

chylothorax ( J:102652 )

• heterozygotes that die within a few days of birth have milky chylous ascites in the peritoneal cavity and/or thoracic cavity

increased liver triglyceride level ( J:102652 )

• triglyceride accumulation is seen in the liver in obese mice

vision/eye

abnormal eye development ( J:83185 )

• after 10 days in culture heterozygous retinal explants have about more cells compared to wild-type explants but fewer than in homozygous explants

abnormal retina morphology ( J:83185 )

• severe retinal dysplasia is seen in older mice degeneration is seen in all 3 retinal layers

increased total retina thickness ( J:83185 )

• in adults the retina is thicker than in wild-type mice

growth/size/body

increased susceptibility to age related obesity ( J:102652 )

• the average weight of 6- to 12-month old heterozygotes is 67.9g compared to 51.8g for wild-type mice

• the most marked weight gain is seen between 9 weeks and 6 months of age

adipose tissue

increased total body fat amount ( J:102652 )

• weight gain is associated with accumulation of the subcutaneous and intra-abdominal fat

increased fat cell size ( J:102652 )

• adipocyte hypertrophy and, in 2 of the most obese mice, hyperplasia are seen

increased total fat pad weight ( J:102652 )

• fat accumulation is most obvious in fat pads around lymph nodes

• individual fat pads in older heterozygotes weigh 2 to 3 times more than wild-type

behavior/neurological

abnormal suckling behavior ( J:102652 )

• many heterozygotes die without suckling

decreased locomotor activity ( J:102652 )

• after the onset of obesity, heterozygotes exercise much less than wild-type mice and tend to eat less; however before the onset of obesity no difference in food consumption or activity is seen

liver/biliary system

increased liver triglyceride level ( J:102652 )

• triglyceride accumulation is seen in the liver in obese mice

hepatic steatosis ( J:102652 )

• 4 of 6 obese mice have fatty livers but no lean mice have fatty livers

hematopoietic system

increased macrophage cell number ( J:102652 )

• an increase number of Mac-2+ macrophages is seen along with liver lipid accumulation

cardiovascular system

abnormal lymph circulation ( J:57646 , J:102652 )

• the intestines are filled with a white fluid (probably chyle) a few hours before death (J:57646)

• leakage of chyle from the mesenteric vessels (J:102652)

• dye injected into the foot pad accumulates in multiple hypoplastic and tortuous lymphatic vessels in the thoracic cavity rather than in the thoracic duct (J:102652)

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
obesity		DOID:9970	OMIM:601665	J:102652

Allelic Composition	Prox1^tm4.1Gco/Prox1⁺
Genetic Background	involves: 129S1/Sv * C57BL/10 * CBA/Ca

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prox1^tm4.1Gco mutation (0 available); any Prox1 mutation (43 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

cardiovascular system

cardiovascular system phenotype ( J:158959 )

N	• embryos show no obvious edema at E15.5

Allelic Composition	Prox1^{tm5(GFP/cre)Gco}/Prox1⁺
Genetic Background	involves: 129S1/Sv * NMRI

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prox1^{tm5(GFP/cre)Gco} mutation (0 available); any Prox1 mutation (43 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

decreased survivor rate ( J:158959 )

• exhibited on this background

• Background Sensitivity: on all other backgrounds tested (no data provided), lethality is nearly 100%

Allelic Composition	Gt(ROSA)26Sor^tm1Sor/Gt(ROSA)26Sor⁺ Nr2f2^tm2.1Tsa/Nr2f2^tm2.1Tsa Prox1^{tm3(cre/ERT2)Gco}/Prox1⁺
Genetic Background	involves: 129S1/Sv * 129S4/SvJaeSor * 129S7/SvEvBrd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sor^tm1Sor mutation (8 available); any Gt(ROSA)26Sor mutation (958 available)
Nr2f2^tm2.1Tsa mutation (0 available); any Nr2f2 mutation (27 available)
Prox1^{tm3(cre/ERT2)Gco} mutation (1 available); any Prox1 mutation (43 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal lymphatic vessel morphology ( J:158959 )

• embryos exposed to tamoxifen at E10.5-12.5 show some blood-filled dermal lymphatic vessels

• embryos exposed to tamoxifen show reduced number of superficial vessels (X-gal stained); severity of reduction increases with early tamoxifen treatment

• tamoxifen treatment at E10.5 or E11.5 results in severely reduced lymphatic endothelial cell numbers and lack of lymphatic vessels

• embryos exposed to tamoxifen at E10.5 or 11.5 display drastically mispatterned lymph sacs that are reduced in size compared to controls

• when tamoxifen exposure occurs at E13.5, few embryos show any lymphatic defects, while exposure later in development or postnatally causes no obvious defects despite reduction in Nr2f2 expression in LECs

homeostasis/metabolism

edema ( J:158959 )

• embryos exposed to tamoxifen at E10.5-12.5 (analyzed at E15.5) display edema

Allelic Composition	Prox1^tm2Gco/Prox1⁺ Tg(Tek-cre)1Ywa/0
Genetic Background	involves: 129S1/Sv * C57BL/6 * NMRI * SJL

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prox1^tm2Gco mutation (0 available); any Prox1 mutation (43 available)
Tg(Tek-cre)1Ywa mutation (6 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

postnatal lethality, incomplete penetrance ( J:102652 )

• most mutants die within a few days of birth but some survive to adulthood

immune system

abnormal lymph circulation ( J:102652 )

• leakage of chyle from the mesenteric vessels is seen in neonates that die postnatally, but not in surviving adults

abnormal lymphatic vessel morphology ( J:102652 )

• lymph vessels are mispatterned and dilated similar to Prox1^tm1Gco heterozygotes

digestive/alimentary system

abnormal intestine morphology ( J:102652 )

• accumulation of lipid is seen in the intestine walls

growth/size/body

increased susceptibility to age related obesity ( J:102652 )

• some mutants display weight gain similar to Prox1^tm1Gco heterozygotes, but the occurrence is decreased

homeostasis/metabolism

edema ( J:102652 )

• at E14.5 edema is seen identical to that in Prox1^tm1Gco heterozygotes

chylothorax ( J:102652 )

• mutants that die within a few days of birth have milky chylous ascites in the peritoneal cavity and thoracic cavity; however, leakage is not seen in surviving adults

cardiovascular system

abnormal lymph circulation ( J:102652 )

• leakage of chyle from the mesenteric vessels is seen in neonates that die postnatally, but not in surviving adults

respiratory system

chylothorax ( J:102652 )

• mutants that die within a few days of birth have milky chylous ascites in the peritoneal cavity and thoracic cavity; however, leakage is not seen in surviving adults

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