Analysis Tools
digestive/alimentary system
| N |
• homozygotes display normal weight gain and stooling pattern with no evidence of histopathologic abnormality along the digestive tract
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• in response to acute chemically-induced colitis, mutants appear to be more affected by 5% dextran sulfate sodium (DSS) than wild-type mice (based on diarrhea, visible blood in stools, weight loss)
• however, 7 days after DSS, homozygotes show no significant differences in these parameters or in colonic histology relative to wild-type mice
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growth/size/body
| N |
• homozygotes are viable, fertile, overtly normal, and display no differences in weight gain relative to wild-type
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hematopoietic system
| N |
• bone marrow aspirates indicate similar myelocytic, erythrocytic, and megakaryocytic lineages in mutant and wild-type mice
• mutants display no differences in the number or morphology of circulating erythrocytes, leukocytes or platelets relative to wild-type
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• in vitro, homozygotes show delayed activation of resident peritoneal macrophages in the first 13 h of incubation with serum; however, activation is gradually increased reaching wild-type levels by 21-24 h
• in vivo, homozygotes show normal migration of mononuclear phagocytes into the peritoneal cavity and normal activation of peritoneal macrophages 72 h after thioglycollate injection
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immune system
| N |
• homozygotes display normal healing of excisional skin wounds relative to wild-type
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• in response to acute chemically-induced colitis, mutants appear to be more affected by 5% dextran sulfate sodium (DSS) than wild-type mice (based on diarrhea, visible blood in stools, weight loss)
• however, 7 days after DSS, homozygotes show no significant differences in these parameters or in colonic histology relative to wild-type mice
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• in vitro, homozygotes show delayed activation of resident peritoneal macrophages in the first 13 h of incubation with serum; however, activation is gradually increased reaching wild-type levels by 21-24 h
• in vivo, homozygotes show normal migration of mononuclear phagocytes into the peritoneal cavity and normal activation of peritoneal macrophages 72 h after thioglycollate injection
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liver/biliary system
| N |
• mutant livers appear macroscopically normal
• in addition, serum biochemical indicators of hepatic synthetic and excretory functions appear unaffected relative to wild-type
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• at 1-6 mo of age, mutant livers display varying degrees of cytoplasmic vacuolization of hepatocytes; all other organs appear normal
• no hepatic inflammation or changes in the bile duct or other nonparenchymal cells are obseved
• cytoplasmic vacuolizations are found in 79% of homozygotes, contain lipid, and are distributed uniformly throughout the liver lobule
• such vacuolizations are detected at 1 month of age and do not appear to progress up to 6 months; however, in some livers the lesion is severe
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