Phenotypes associated with this allele

• Allele Symbol: Pmch^tm1Emf
• Allele Name: targeted mutation 1, Eleftheria Maratos-Flier
• Allele ID: MGI:2445313
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pmch^tm1Emf/Pmch^tm1Emf	involves: 129S4/SvJae * C57BL/6	MGI:3041645
cx2	Lep^ob/Lep^ob Pmch^tm1Emf/Pmch^tm1Emf	involves: 129S4/SvJae * C57BL/6	MGI:3041647

Genotype
MGI:3041645

hm1

• Allelic Composition: Pmch^tm1Emf/Pmch^tm1Emf
• Genetic Background: involves: 129S4/SvJae * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

decreased food intake ( J:51659 )

• hypophagia - 6 week old mice ingested 12% fewer calories over a 24 hour period than did controls

growth/size/body

decreased body weight ( J:51659 )

• reduced size became apparent around the 4th to 5th week

• at 17 weeks males weighed 28% less and females weighed 24% less than controls

• body length was normal at 16 weeks

increased susceptibility to weight loss ( J:51659 )

• weight loss during starvation greater than in controls

• after 48 hours of starvation 3 out of 4 mice died wheras none of the controls died

• hyperphagic response to starvation was normal

homeostasis/metabolism

decreased circulating leptin level ( J:51659 )

• decreased leptin levels in the body

increased oxygen consumption ( J:51659 )

• body weight reduction greater than expected from reduction in food intake

• oxygen consumption increased about 20% when normalized to body mass

decreased triglyceride level ( J:51659 )

• body content of triglycerides reduced

Genotype
MGI:3041647

cx2

• Allelic Composition: Lep^ob/Lep^ob; Pmch^tm1Emf/Pmch^tm1Emf
• Genetic Background: involves: 129S4/SvJae * C57BL/6

adipose tissue

abnormal adipose tissue amount ( J:85176 )

• considerably less fat tissue than Lep^ob homozygous mice

behavior/neurological

increased food intake ( J:85176 )

• food intake about 3X wild-type

decreased locomotor activity ( J:85176 )

• much less active than wild-type mice but 3X as active as mice homozygous only for Lep^ob

growth/size/body

increased body weight ( J:85176 )

• heavier than wild-type but weighed less than mice homozygous only for Lep^ob

homeostasis/metabolism

impaired adaptive thermogenesis ( J:85176 )

• slower and smaller drop in body temperature with cold exposure

abnormal circulating corticosterone level ( J:85176 )

• levels 2X wild-type mice but half of levels in mice homozygous only for Lep^ob

increased oxygen consumption ( J:85176 )

abnormal glucose homeostasis ( J:85176 )

decreased circulating glucose level ( J:85176 )

• fasting glucose levels were significantly decreased

increased circulating insulin level ( J:85176 )

abnormal glucose tolerance ( J:85176 )

• glucose levels ranged from 11.5-31.6% lower than in mice homozygous only for Lep^ob

liver/biliary system

hepatic steatosis ( J:85176 )

• hepatosteatosis and heavy livers