Phenotypes associated with this allele

• Allele Symbol: Rapgef1^{Gt(pGT1.8geo)5Pgr}
• Allele Name: gene trap 5, Peter Gruss
• Allele ID: MGI:2445403
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rapgef1^Gt(pGT1.8geo)5Pgr/Rapgef1^Gt(pGT1.8geo)5Pgr	involves: 129	MGI:3722119
hm2	Rapgef1^Gt(pGT1.8geo)5Pgr/Rapgef1^Gt(pGT1.8geo)5Pgr	involves: 129/Sv * C57BL/6 * Swiss	MGI:3722116

Genotype
MGI:3722119

hm1

• Allelic Composition: Rapgef1^{Gt(pGT1.8geo)5Pgr}/Rapgef1^{Gt(pGT1.8geo)5Pgr}
• Genetic Background: involves: 129

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal embryonic neuroepithelium morphology ( J:119299 )

• at E12.5, the cerebrocortical neuroepithelium is enlarged but differentiation fields are reduced in size

• at E12.5, cell density of the ventricular proliferation zone is increased

• at E14.5, cerebral neuroepithelium is expanded or organized in folds

• at E14.5, the differentiation zone overlying the ventricular zone is reduced in size and volume

• cells in the neuroepithelium exhibit overproliferation and continue to divide longer than in wild-type mice

embryo

abnormal embryonic neuroepithelium morphology ( J:119299 )

• at E12.5, the cerebrocortical neuroepithelium is enlarged but differentiation fields are reduced in size

• at E12.5, cell density of the ventricular proliferation zone is increased

• at E14.5, cerebral neuroepithelium is expanded or organized in folds

• at E14.5, the differentiation zone overlying the ventricular zone is reduced in size and volume

• cells in the neuroepithelium exhibit overproliferation and continue to divide longer than in wild-type mice

Genotype
MGI:3722116

hm2

• Allelic Composition: Rapgef1^{Gt(pGT1.8geo)5Pgr}/Rapgef1^{Gt(pGT1.8geo)5Pgr}
• Genetic Background: involves: 129/Sv * C57BL/6 * Swiss

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:80219 )

• mice begin to die at E10.5 and by E14.5 all mice are dead

cardiovascular system

abnormal brain vasculature morphology ( J:80219 )

• hemorrhaging initiates in the small cephalic blood vessels often in the area of the hindbrain neural epithelium close to the olfactory epithelium

• few to no smooth muscle alpha-actin positive cells are associated with the brain vasculature

abnormal vascular smooth muscle morphology ( J:80219 )

• smooth muscle alpha-actin positive cells for less compact rings around major vessels

vascular smooth muscle hypoplasia ( J:80219 )

• few to no smooth muscle alpha-actin positive cells are associated with the brain vasculature

hemorrhage ( J:80219 )

• at E10.5 and E11.5, mice exhibit hemorrhaging into the lumen of the neural tube

• at E14.5, dying mice exhibit hemorrhagic edemas

• hemorrhaging initiates in the small cephalic blood vessels often in the area of the hindbrain neural epithelium close to the olfactory epithelium

nervous system

abnormal nervous system morphology ( J:80219 )

• mice exhibit abnormal nervous system morphology

abnormal brain vasculature morphology ( J:80219 )

• hemorrhaging initiates in the small cephalic blood vessels often in the area of the hindbrain neural epithelium close to the olfactory epithelium

• few to no smooth muscle alpha-actin positive cells are associated with the brain vasculature

cellular

abnormal cell adhesion ( J:80219 )

• mouse embryonic fibroblast cells derived from E10.5 embryos exhibit reduced adhesion to laminin and gelatine

• however, adhesion to fibronectin is unaffected

decreased fibroblast cell migration ( J:80219 )

• primary fibroblast cells derived from E10.5 embryos exhibit reduced cell migration

homeostasis/metabolism

edema ( J:80219 )

• at E14.5, dying mice exhibit edema and hemorrhagic edemas

muscle

abnormal vascular smooth muscle morphology ( J:80219 )

• smooth muscle alpha-actin positive cells for less compact rings around major vessels

vascular smooth muscle hypoplasia ( J:80219 )

• few to no smooth muscle alpha-actin positive cells are associated with the brain vasculature