Phenotypes associated with this allele

• Allele Symbol: Dad1^tm1Brw
• Allele Name: targeted mutation 1, Jay L Brewster
• Allele ID: MGI:2445490
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Dad1^tm1Brw/Dad1^tm1Brw	involves: 129P2/OlaHsd * Black Swiss	MGI:3639723
ht2	Dad1^tm1Brw/Dad1^+	involves: 129P2/OlaHsd * Black Swiss	MGI:3639729

Genotype
MGI:3639723

hm1

• Allelic Composition: Dad1^tm1Brw/Dad1^tm1Brw
• Genetic Background: involves: 129P2/OlaHsd * Black Swiss

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:62083 )

• homozygous mutant embryos die early in development between E3.5 and E6.5

embryo

abnormal inner cell mass apoptosis ( J:62083 )

• upon culture, E7.5 homozygous mutant embryos show a dramatic increase in apoptotic cell death within the inner cell mass relative to wild-type and heterozygous embryos (5.3-fold and 2.6-fold, respectively)

abnormal inner cell mass morphology ( J:62083 )

• in culture, E7.5 homozygous mutant embryos display a reduced inner cell mass relative to wild-type embryos

cellular

abnormal inner cell mass apoptosis ( J:62083 )

• upon culture, E7.5 homozygous mutant embryos show a dramatic increase in apoptotic cell death within the inner cell mass relative to wild-type and heterozygous embryos (5.3-fold and 2.6-fold, respectively)

Genotype
MGI:3639729

ht2

• Allelic Composition: Dad1^tm1Brw/Dad1⁺
• Genetic Background: involves: 129P2/OlaHsd * Black Swiss

mortality/aging

mortality/aging ( J:62083 )

N • upon culture, E7.5 heterozygous mutant embryos are slightly under-represented relative to wild-type embryos; however, a normal Mendelian ratio of 2:1 is observed in vivo at weaning

cellular

increased embryonic tissue cell apoptosis ( J:62083 )

• in culture, E7.5 heterozygous mutant embryos show a 2-fold increase in apoptotic cell death relative to wild-type embryos, with variable involvement; however, this difference does not reach statistical significance

embryo

increased embryonic tissue cell apoptosis ( J:62083 )

• in culture, E7.5 heterozygous mutant embryos show a 2-fold increase in apoptotic cell death relative to wild-type embryos, with variable involvement; however, this difference does not reach statistical significance