Phenotypes associated with this allele

• Allele Symbol: Dkc1^tm1Bsl
• Allele Name: targeted mutation 1, Monica Bessler
• Allele ID: MGI:2446497
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Dkc1^tm1Bsl/Dkc1^tm1Bsl	involves: 129/Sv * C57BL/6	MGI:3778161
cn2	Dkc1^tm1Bsl/Y Tg(Gata1-cre)1Sho/0	involves: 129/Sv * C57BL/6 * CD-1	MGI:3778208
cn3	Dkc1^tm1Bsl/Dkc1^+ Tg(Gata1-cre)1Sho/0	involves: 129/Sv * C57BL/6 * CD-1	MGI:3778210
ot4	Dkc1^tm1Bsl/Y	involves: 129/Sv * C57BL/6	MGI:3778162

Genotype
MGI:3778161

hm1

• Allelic Composition: Dkc1^tm1Bsl/Dkc1^tm1Bsl
• Genetic Background: involves: 129/Sv * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:80494 )

♀ • mice are viable and fertile and indistinguishable from wild-type littermates

Genotype
MGI:3778208

cn2

• Allelic Composition: Dkc1^tm1Bsl/Y; Tg(Gata1-cre)1Sho/0
• Genetic Background: involves: 129/Sv * C57BL/6 * CD-1

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:80494 )

♂ • no male embryos are recovered at E7.5 when the mutant allele is derived maternally

♂ • same phenotype is observed when EIIa-cre transgenic males are used

Genotype
MGI:3778210

cn3

• Allelic Composition: Dkc1^tm1Bsl/Dkc1⁺; Tg(Gata1-cre)1Sho/0
• Genetic Background: involves: 129/Sv * C57BL/6 * CD-1

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:80494 )

♀ • no live embryos are recovered at E9.5 and most have been resorbed at E12.5, with inheritance of the maternally derived mutant allele

♀ • same phenotype is observed when EIIa-cre transgenic males are used

embryo

increased trophectoderm apoptosis ( J:80494 )

♀ • after E7.5, apoptotic nuclei are observed in the trophectoderm

abnormal embryo development ( J:80494 )

♀ • at E8.5 and E9.5, embryos appear morphologically disorganized relative to wild-type

failure of initiation of embryo turning ( J:80494 )

♀ • at E8.5 or E9.5, embryos do not show signs of axial rotation

embryonic growth arrest ( J:80494 )

♀ • after E7.5, embryos become developmentally growth arrested

decreased embryo size ( J:80494 )

♀ • at E8.5 and E9.5, embryos are visibly smaller than littermates

trophectoderm cell degeneration ( J:80494 )

• at E8.5 and E9.5, degeneration within the trophectoderm becomes more extensive

growth/size/body

decreased embryo size ( J:80494 )

♀ • at E8.5 and E9.5, embryos are visibly smaller than littermates

reproductive system

transmission ratio distortion ( J:80494 )

♀ • females with the deletion on the paternally-inherited allele are unable to transmit the deletion to offspring; only homozygous Dkc1⁺, cre transgene-negative male and female offspring are obtained when females heterozygous for the deleted allele are crossed to wild-type males

cellular

increased trophectoderm apoptosis ( J:80494 )

♀ • after E7.5, apoptotic nuclei are observed in the trophectoderm

Genotype
MGI:3778162

ot4

• Allelic Composition: Dkc1^tm1Bsl/Y
• Genetic Background: involves: 129/Sv * C57BL/6

normal phenotype

no abnormal phenotype detected ( J:80494 )

♂ • mice are viable and fertile and indistinguishable from wild-type littermates