All Phenotypes Ltb/Tnf<tm1.1Dvk> MGI Mouse

absent germinal center B cells ( J:58981 )

• germinal centers do not form in the spleen

abnormal spleen morphology ( J:58981 )

• spleen architecture is more disrupted than in Ltb^tm1Kpf homozygotes

intermingled spleen red and white pulp ( J:58981 )

absent spleen marginal zone ( J:58981 )

• the marginal zone in the spleen is absent and T and B cell areas are variable

absent follicular dendritic cells ( J:58981 )

• mature follicular dendritic cells are absent from the mesenteric lymph nodes

absent Peyer's patches ( J:58981 )

abnormal mesenteric lymph node morphology ( J:58981 )

• in most mice only one large spherical mesenteric lymph node is present unlike in wild-type mice that possess a chain of three elongated lymph node segments

• mature follicular dendritic cells are absent from the mesenteric lymph nodes

• unlike in Ltb^tm1Kpf homozygotes and wild-type mice, PNA+ B cells do not aggregate but remain scattered

• the reticulum of the deep cortex is uniform and coarser than in wild-type mice and there is no transition to germinal center-like structures

absent axillary lymph nodes ( J:58981 )

absent brachial lymph nodes ( J:58981 )

absent cervical lymph nodes ( J:58981 )

• cervical lymph nodes could not be identified

absent inguinal lymph nodes ( J:58981 )

absent popliteal lymph nodes ( J:58981 )

absent peripheral lymph nodes ( J:58981 )

• mice lack brachial, iliac, axillary, inguinal and popliteal lymph nodes

• however, mesenteric and sacral lymph nodes are present