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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Pik3cdtm1Jni
targeted mutation 1, James N Ihle
MGI:2446574
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Pik3cdtm1Jni/Pik3cdtm1Jni involves: 129P2/OlaHsd MGI:3796414
hm2
 		Pik3cdtm1Jni/Pik3cdtm1Jni involves: 129P2/OlaHsd * C57BL/6 MGI:3796420
cx3
 		Pik3cdtm1Jni/Pik3cdtm1Jni
Pik3cgtm1Pngr/Pik3cgtm1Pngr 		involves: 129P2/OlaHsd * C57BL/6 MGI:3796421


Genotype
MGI:3796414
hm1
Allelic
Composition		 Pik3cdtm1Jni/Pik3cdtm1Jni
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pik3cdtm1Jni mutation (0 available); any Pik3cd mutation (45 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
homeostasis/metabolism
abnormal B cell calcium ion homeostasis ( J:80614 )
• stimulated B cells only product an increase in intracellular calcium that is 25% of that observed in wild-type cells

immune system
decreased B cell number ( J:80614 )
• the total number of bone marrow B-lineage cells is decreased 25% compared to in wild-type mice
decreased mature B cell number ( J:80614 )
• mice have fewer B220+CD43-, B220+IgM+ and B220++IgMlow mature B cells compared to in wild-type mice
• mice exhibit reduced total B220+ splenocytes compared to in wild-type mice
absent B-1 B cells ( J:80614 )
• mice lack CD5+ B1 B cells in the peritoneal lymphocytes
abnormal B cell calcium ion homeostasis ( J:80614 )
• stimulated B cells only product an increase in intracellular calcium that is 25% of that observed in wild-type cells
abnormal humoral immune response ( J:80614 )
• IgM and IgG1 response to TNP-LPS treatment is 30% to 50% lower than in wild-type mice
• IgM, IgG1, IgG2b and IgG3 response to TNP-Ficoll is lower than in wild-type mice
• mice immunized with TNP-KLH fail to mount any response unlike wild-type mice
decreased immunoglobulin level ( J:80614 )
• mice exhibit lower levels of all immunoglobulins, with IgM and IgG1 levels the most decreased, compared to in wild-type mice

hematopoietic system
decreased B cell number ( J:80614 )
• the total number of bone marrow B-lineage cells is decreased 25% compared to in wild-type mice
decreased mature B cell number ( J:80614 )
• mice have fewer B220+CD43-, B220+IgM+ and B220++IgMlow mature B cells compared to in wild-type mice
• mice exhibit reduced total B220+ splenocytes compared to in wild-type mice
absent B-1 B cells ( J:80614 )
• mice lack CD5+ B1 B cells in the peritoneal lymphocytes
abnormal B cell calcium ion homeostasis ( J:80614 )
• stimulated B cells only product an increase in intracellular calcium that is 25% of that observed in wild-type cells
decreased immunoglobulin level ( J:80614 )
• mice exhibit lower levels of all immunoglobulins, with IgM and IgG1 levels the most decreased, compared to in wild-type mice

cellular




Genotype
MGI:3796420
hm2
Allelic
Composition		 Pik3cdtm1Jni/Pik3cdtm1Jni
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pik3cdtm1Jni mutation (0 available); any Pik3cd mutation (45 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:124330 )
N
• splenic NK cells are phenotypically indistinguishable from wild-type




Genotype
MGI:3796421
cx3
Allelic
Composition		 Pik3cdtm1Jni/Pik3cdtm1Jni
Pik3cgtm1Pngr/Pik3cgtm1Pngr
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pik3cdtm1Jni mutation (0 available); any Pik3cd mutation (45 available)
Pik3cgtm1Pngr mutation (0 available); any Pik3cg mutation (59 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal NK cell differentiation ( J:124330 )
• mice exhibit a high percentage of CD117+ and CD27+ NK cells and about 40% to 50% immature CD27 CD11b- cells compared to in wild-type mice
• NK cell development is blocked before the stage at which they acquire Ly49 receptors
decreased NK cell number ( J:124330 )
• mice exhibit a 60% to 70% reduction in NK cells in the spleen compared to in wild-type mice
decreased T cell number ( J:124330 )
• CD3+ T cell numbers and percentages are reduced compared to in wild-type mice
spleen hypoplasia ( J:124330 )
• total splenocyte numbers are reduced 50% compared to in wild-type mice
decreased cytotoxic T cell cytolysis ( J:124330 )
• NK cells are less lytic than wild-type cells
decreased interferon-gamma secretion ( J:124330 )
• mice exhibit decreased IFN-gamma secretion following NK1.1 or IL-12/IL-18 stimulation compared to in wild-type mice

hematopoietic system
abnormal NK cell differentiation ( J:124330 )
• mice exhibit a high percentage of CD117+ and CD27+ NK cells and about 40% to 50% immature CD27 CD11b- cells compared to in wild-type mice
• NK cell development is blocked before the stage at which they acquire Ly49 receptors
decreased NK cell number ( J:124330 )
• mice exhibit a 60% to 70% reduction in NK cells in the spleen compared to in wild-type mice
decreased T cell number ( J:124330 )
• CD3+ T cell numbers and percentages are reduced compared to in wild-type mice
spleen hypoplasia ( J:124330 )
• total splenocyte numbers are reduced 50% compared to in wild-type mice
decreased cytotoxic T cell cytolysis ( J:124330 )
• NK cells are less lytic than wild-type cells




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory