Phenotypes associated with this allele

• Allele Symbol: Tg(Tek-GFP/Cbfb)2Spe
• Allele Name: transgene insertion 2, Nancy A Speck
• Allele ID: MGI:2447351
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Cbfb^tm1Spe/Cbfb^tm1Spe Tg(Tek-GFP/Cbfb)2Spe/0	either: (involves: 129S4/SvJae * BALB/c * CD-1 * Swiss Webster) or (involves: 129S4/SvJae * C57BL/6 * CD-1 * Swiss Webster)	MGI:2653204

Genotype
MGI:2653204

cx1

• Allelic Composition: Cbfb^tm1Spe/Cbfb^tm1Spe; Tg(Tek-GFP/Cbfb)2Spe/0
• Genetic Background: either: (involves: 129S4/SvJae * BALB/c * CD-1 * Swiss Webster) or (involves: 129S4/SvJae * C57BL/6 * CD-1 * Swiss Webster)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

abnormal osteoblast differentiation ( J:80670 )

• bone collar shows few differentiating osteoblasts

mortality/aging

perinatal lethality, complete penetrance ( J:80670 )

• die at birth

craniofacial

protruding tongue ( J:80670 )

short snout ( J:80670 )

hematopoietic system

small thymus ( J:80670 )

• thymus is small and contains 50% fewer cells than in wild-type

abnormal definitive hematopoiesis ( J:80670 )

abnormal common myeloid progenitor cell morphology ( J:80670 )

• fetal livers contain 50% as many colony forming unit (CFU)-granulocyte/erythroid/monocyte/macrophage (GEMM) progenitors as do wild-type

abnormal erythropoiesis ( J:80670 )

• overall increase in the proportion of red blood cell precursors from 38% to 79% of nucleated cells at E17.5

abnormal myelopoiesis ( J:80670 )

• few mature myeloid cells are present

increased erythroid progenitor cell number ( J:80670 )

• ratio of erythroid precursors to myeloid precursors is increased from 0.48 in wild-type fetuses to 1.22 in mutant fetuses at E17.5

increased CD4-positive, alpha-beta T cell number ( J:80670 )

• increase in numbers of single CD4+ cells in thymuses

increased hematopoietic stem cell number ( J:80670 )

• 2-fold increase in hematopoietic progenitors

spleen hypoplasia ( J:80670 )

• spleens are hypocellular, but overall structure and organization is normal

abnormal splenic cell ratio ( J:80670 )

• fewer B220+ cells, GR-1+ neutrophils and Mac-1+ cells in E17.5 spleens

immune system

small thymus ( J:80670 )

• thymus is small and contains 50% fewer cells than in wild-type

abnormal myelopoiesis ( J:80670 )

• few mature myeloid cells are present

increased CD4-positive, alpha-beta T cell number ( J:80670 )

• increase in numbers of single CD4+ cells in thymuses

spleen hypoplasia ( J:80670 )

• spleens are hypocellular, but overall structure and organization is normal

abnormal splenic cell ratio ( J:80670 )

• fewer B220+ cells, GR-1+ neutrophils and Mac-1+ cells in E17.5 spleens

skeleton

abnormal osteoblast differentiation ( J:80670 )

• bone collar shows few differentiating osteoblasts

bowed tibia ( J:80670 )

abnormal long bone hypertrophic chondrocyte zone ( J:80670 )

delayed chondrocyte differentiation ( J:80670 )

• delay in chondrocyte maturation

delayed bone ossification ( J:80670 )

• ossification of the limbs, ribs and vertebra is delayed at E17.5

delayed endochondral bone ossification ( J:80670 )

delayed intramembranous bone ossification ( J:80670 )

• intramembranous bone formation is initiated but is either prematurely terminated or delayed at an early stage of osteoblast differentiation

vision/eye

eyelids open at birth ( J:80670 )

• delay in or incomplete closure of the eyelids

digestive/alimentary system

protruding tongue ( J:80670 )

limbs/digits/tail

bowed tibia ( J:80670 )

short limbs ( J:80670 )

liver/biliary system

abnormal liver morphology ( J:80670 )

• decrease in the percentage of CD19+ and B220+ B cells, GR1+ neutrophils and Mac-1+ cells in fetal liver

endocrine/exocrine glands

small thymus ( J:80670 )

• thymus is small and contains 50% fewer cells than in wild-type

growth/size/body

protruding tongue ( J:80670 )

short snout ( J:80670 )