Phenotypes associated with this allele

• Allele Symbol: Npy2r^tm1.1Hhz
• Allele Name: targeted mutation 1.1, Herbert Herzog
• Allele ID: MGI:2447398
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Npy2r^tm1.1Hhz/Npy2r^tm1.1Hhz	involves: 129X1/SvJ	MGI:3771169
hm2	Npy2r^tm1.1Hhz/Npy2r^tm1.1Hhz	involves: 129X1/SvJ * BALB/cJ * C57BL/6	MGI:4361941
hm3	Npy2r^tm1.1Hhz/Npy2r^tm1.1Hhz	involves: 129X1/SvJ * C57BL/6	MGI:3693951
cx4	Npy2r^tm1.1Hhz/Npy2r^tm1.1Hhz Npy4r^tm1.1Hhz/Npy4r^tm1.1Hhz	involves: 129X1/SvJ * BALB/cJ * C57BL/6	MGI:4361940
cx5	Lep^ob/Lep^ob Npy2r^tm1.1Hhz/Npy2r^tm1.1Hhz	involves: 129X1/SvJ * C57BL/6	MGI:3694012
cx6	Npy2r^tm1.1Hhz/Npy2r^tm1.1Hhz Npy4r^tm1.1Hhz/Npy4r^tm1.1Hhz	involves: 129X1/SvJ * C57BL/6	MGI:4362036

Genotype
MGI:3771169

hm1

• Allelic Composition: Npy2r^tm1.1Hhz/Npy2r^tm1.1Hhz
• Genetic Background: involves: 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

abnormal food intake ( J:129742 )

• mice are resistant to satiating and weight-reducing effects of high-protein diets

Genotype
MGI:4361941

hm2

• Allelic Composition: Npy2r^tm1.1Hhz/Npy2r^tm1.1Hhz
• Genetic Background: involves: 129X1/SvJ * BALB/cJ * C57BL/6

skeleton

decreased osteoclast cell number ( J:84570 )

abnormal trabecular bone morphology ( J:84570 )

• mice exhibit an increase in cancellous bone volume, trabecular number, and trabecular thickness compared with wild-type mice

increased trabecular bone thickness ( J:84570 )

adipose tissue

decreased white adipose tissue amount ( J:84570 )

• in male mice

behavior/neurological

abnormal eating behavior ( J:84570 )

• in male mice

growth/size/body

decreased body weight ( J:84570 )

• in male mice

homeostasis/metabolism

increased circulating pancreatic peptide level ( J:84570 )

• plasma pancreatic peptide levels are increased compared to in wild-type mice

hematopoietic system

decreased osteoclast cell number ( J:84570 )

immune system

decreased osteoclast cell number ( J:84570 )

Genotype
MGI:3693951

hm3

• Allelic Composition: Npy2r^tm1.1Hhz/Npy2r^tm1.1Hhz
• Genetic Background: involves: 129X1/SvJ * C57BL/6

mortality/aging

increased susceptibility to xenobiotic induced morbidity/mortality ( J:124729 )

• kainic acid-exposed mice over-expressing rAAV1/2-NPY exhibit an increase in mortality compared with similarly treated wild-type mice

behavior/neurological

increased susceptibility to pharmacologically induced seizures ( J:124729 )

• mice exhibit a higher proportion of time in motor seizures induced by the over-expression of rAAV1/2-NPY compared with wild-type mice

• however, mice expressing rAAV1/2 empty vector exhibit normal seizure response

• kainic acid-exposed mice over-expressing rAAV1/2-NPY exhibit an increase in mortality compared with similarly treated wild-type mice

impaired long-term object recognition memory ( J:121312 )

• 1 and 6 hours after initial exposure, mice exhibit deteriorating object recognition compared with wild-type mice

abnormal spatial learning ( J:121312 )

• in a probe trial, mice make fewer passes over and spend less time at the original platform position compared with similarly treated wild-type mice

• however, swim speed and visible platform performance are normal

increased fluid intake ( J:115774 )

• mutants drink significantly more during the dark phase compared to controls

• total 24 hour water intake is significantly increased during the 3 day observation period

• this behavior is maintained following injection of physiological saline (vehicle). beta-adrenoreceptor antagonist propanolol, or angiotensin AT1 receptor antagonist telmisartan

abnormal eating behavior ( J:77345 )

• in male mice at 8 weeks but not 12 weeks

• in female mice at 8 and 12 weeks

decreased anxiety-related response ( J:96222 )

• in an elevated plus maze, mice make more entries and spend more time in open arms compared with wild-type mice

• in an open field test, mice exhibit a preference for the central area unlike wild-type mice

homeostasis/metabolism

decreased circulating corticosterone level ( J:107169 )

• level is attenuated compared to wild-type (64 ng/ml vs 102 ng/ml in wild-type)

decreased circulating insulin level ( J:77345 )

♂ • in corticosterone treated male mice

increased circulating insulin-like growth factor I level ( J:129609 )

• after fasting compared with similarly treated wild-type mice

increased circulating pancreatic peptide level ( J:77345 )

♂ • increased 3-fold in male mice

increased growth hormone level ( J:129609 )

• pituitary gland cell growth hormone levels are increased in fasting mice compared to non-fasting mice and unlike in similarly treated wild-type mice

abnormal testosterone level ( J:129609 )

• testosterone levels in Leydig cells are increased in fasting mice compared to in similarly treated wild-type mice

increased susceptibility to xenobiotic induced morbidity/mortality ( J:124729 )

• kainic acid-exposed mice over-expressing rAAV1/2-NPY exhibit an increase in mortality compared with similarly treated wild-type mice

nervous system

increased susceptibility to pharmacologically induced seizures ( J:124729 )

• mice exhibit a higher proportion of time in motor seizures induced by the over-expression of rAAV1/2-NPY compared with wild-type mice

• however, mice expressing rAAV1/2 empty vector exhibit normal seizure response

• kainic acid-exposed mice over-expressing rAAV1/2-NPY exhibit an increase in mortality compared with similarly treated wild-type mice

abnormal olfactory sensory neuron morphology ( J:132859 )

• fewer CR+ neurons are located in the glomerular and granular cell layers of the olfactory bulb compared to in wild-type mice

• fewer CB+ and TH+ neurons are located in the glomerular layer of the olfactory bulb compared to in wild-type mice

abnormal rostral migratory stream morphology ( J:132859 )

• fewer proliferating cells are found in the subventricular zone and migratory rostral stream compared to in wild-type mice

• fewer DC+ cells are located in the subventricular zone and rostral migratory stream than in wild-type mice

abnormal postnatal subventricular zone morphology ( J:132859 )

• fewer proliferating cells are found in the subventricular zone leading to fewer migrating neuroblasts compared to in wild-type mice

• fewer DC+ cells are located in the subventricular zone and rostral migratory stream than in wild-type mice

decreased neuronal precursor cell number ( J:132859 )

• fewer proliferating cells are found in the subventricular zone and migratory rostral stream compared to in wild-type mice

• fewer DC+ cells are located in the subventricular zone and rostral migratory stream than in wild-type mice

skeleton

abnormal trabecular bone morphology ( J:97455 )

• trabecular bone volume is increased 2-fold compared to in wild-type mice

• trabeculae number and thickness are increased compared to in wild-type mice

increased trabecular bone thickness ( J:97455 )

growth/size/body

abnormal olfactory sensory neuron morphology ( J:132859 )

• fewer CR+ neurons are located in the glomerular and granular cell layers of the olfactory bulb compared to in wild-type mice

• fewer CB+ and TH+ neurons are located in the glomerular layer of the olfactory bulb compared to in wild-type mice

decreased body weight ( J:77345 )

• beginning at 4 weeks of age and continuing for the 16 week monitoring period

adipose tissue

decreased white adipose tissue amount ( J:77345 )

♂ • in male mice regardless of corticosterone treatment

craniofacial

abnormal olfactory sensory neuron morphology ( J:132859 )

• fewer CR+ neurons are located in the glomerular and granular cell layers of the olfactory bulb compared to in wild-type mice

• fewer CB+ and TH+ neurons are located in the glomerular layer of the olfactory bulb compared to in wild-type mice

respiratory system

abnormal olfactory sensory neuron morphology ( J:132859 )

• fewer CR+ neurons are located in the glomerular and granular cell layers of the olfactory bulb compared to in wild-type mice

• fewer CB+ and TH+ neurons are located in the glomerular layer of the olfactory bulb compared to in wild-type mice

taste/olfaction

abnormal olfactory sensory neuron morphology ( J:132859 )

• fewer CR+ neurons are located in the glomerular and granular cell layers of the olfactory bulb compared to in wild-type mice

• fewer CB+ and TH+ neurons are located in the glomerular layer of the olfactory bulb compared to in wild-type mice

Genotype
MGI:4361940

cx4

• Allelic Composition: Npy2r^tm1.1Hhz/Npy2r^tm1.1Hhz; Npy4r^tm1.1Hhz/Npy4r^tm1.1Hhz
• Genetic Background: involves: 129X1/SvJ * BALB/cJ * C57BL/6

skeleton

abnormal osteoblast physiology ( J:84570 )

• osteoblast bone formation is increased compared to in wild-type mice

abnormal bone structure ( J:84570 )

• periosteal diameter of the cortical shaft is reduced compared to in wild-type mice

decreased compact bone thickness ( J:84570 )

• cortical bone area and thickness are decreased compared to in wild-type mice

abnormal trabecular bone morphology ( J:84570 )

• mice exhibit an increase in cancellous bone volume, trabeculae number, and trabecular thickness compared with wild-type mice and single homozygotes

increased trabecular bone thickness ( J:84570 )

abnormal bone ossification ( J:84570 )

• mice exhibit increased bone turnover with osteoblast, osteoclast, and osteoid surfaces increased compared with wild-type mice and single homozygotes

• however, osteoblast and osteoclast numbers are normal

abnormal bone mineralization ( J:84570 )

• mineral apposition rate is increased compared to in wild-type mice

homeostasis/metabolism

decreased circulating insulin level ( J:84570 )

♂ • in male mice compared with wild-type mice

decreased circulating leptin level ( J:84570 )

♂ • in male mice compared with wild-type mice and Ppyr1^tm1.1Hhz homozygotes

increased circulating pancreatic peptide level ( J:84570 )

• plasma pancreatic peptide levels are increased compared to in wild-type mice

adipose tissue

decreased brown adipose tissue amount ( J:84570 )

decreased white adipose tissue amount ( J:84570 )

♂ • male mice exhibit a decreased in white adipose tissue compared to in wild-type mice or single homozygotes

growth/size/body

decreased body weight ( J:84570 )

♂ • male mice exhibit decreased body weight compared with wild-type mice that is not as severe as in single homozygotes

behavior/neurological

abnormal eating behavior ( J:84570 )

♂ • in male mice compared to in wild-type mice and single homozygotes

cellular

abnormal osteoblast physiology ( J:84570 )

• osteoblast bone formation is increased compared to in wild-type mice

Genotype
MGI:3694012

cx5

• Allelic Composition: Lep^ob/Lep^ob; Npy2r^tm1.1Hhz/Npy2r^tm1.1Hhz
• Genetic Background: involves: 129X1/SvJ * C57BL/6

growth/size/body

increased pancreas weight ( J:107169 )

• 0.37 g vs 0.29 in wild-type

decreased lean body mass ( J:107169 )

• decreased (19.8 g, 43% of body weight) compared to wild-type (25.5 g 87% of body weight); lean mass is increased significantly compared to Lep homozygotes (16 g, 33% of body weight)

increased body weight ( J:107169 )

• higher than wild-type and similar to in Lep^ob homozygotes

increased kidney weight ( J:107169 )

• 0.46 g vs 0.41 g in wild-type; size is reduced compared to Lep homozygotes

increased liver weight ( J:107169 )

• 4.45 g vs 1.44 g in wild-type

homeostasis/metabolism

abnormal circulating glucose level ( J:107169 )

• levels are similar to wild-type, but greatly decreased from levels found in Lep^ob homozygotes

decreased circulating testosterone level ( J:107169 )

• significantly lower (5.2 nmol/l) compared to wild-type (14.2 nmol/l)

decreased circulating corticosterone level ( J:107169 )

• levels are decreased compared to Lep homozygotes, and normalized compared to wild-type levels

abnormal circulating insulin level ( J:107169 )

• circulating insulin levels are not significantly higher than wild-type but are significantly decreased from Lep^ob homozygotes

increased circulating cholesterol level ( J:107169 )

• 6.56 mmol/l vs 3.54 mmol/l in wild-type

increased circulating triglyceride level ( J:107169 )

• 2.86 mmol/l vs 1.62 mmol/l in wild-type

behavior/neurological

polyphagia ( J:107169 )

endocrine/exocrine glands

increased pancreas weight ( J:107169 )

• 0.37 g vs 0.29 in wild-type

decreased testis weight ( J:107169 )

♂ • testes weigh 0.20 g compared to 0.33 g in wild-type

adipose tissue

abnormal adipose tissue amount ( J:107169 )

• combined white adipose tissue mass (WAT) is higher than wild-type but significantly lower than in Lep^ob homozygotes (WAT % body weight - wild-type 2.32%, Lep - 8.01, Npy2r, Lep double homozygotes - 6.88%)

decreased epididymal fat pad weight ( J:107169 )

• epididymal fat depot weight is significantly decreased compared to those of Lep^ob homozygotes

decreased mesenteric fat pad weight ( J:107169 )

• mesenteric fat depot weight is significantly decreased compared to those of Lep^ob homozygotes

reproductive system

decreased testis weight ( J:107169 )

♂ • testes weigh 0.20 g compared to 0.33 g in wild-type

female infertility ( J:76479 )

♀ • no females can produce offspring

male infertility ( J:76479 , J:107169 )

♂ • no males can produce offspring (J:76479)

♂ • no males could produce offspring (J:107169)

digestive/alimentary system

abnormal intestine morphology ( J:107169 )

• intestinal hypertrophy is attenuated compared to Lep^ob homozygotes (weight - 1.61 g vs 1.89 g in Lep^ob mice) but is still much greater than wild-type (1.61 g vs 1.03 g; intestine length 43 cm vs 33 cm in wild-type)

liver/biliary system

increased liver weight ( J:107169 )

• 4.45 g vs 1.44 g in wild-type

renal/urinary system

increased kidney weight ( J:107169 )

• 0.46 g vs 0.41 g in wild-type; size is reduced compared to Lep homozygotes

Genotype
MGI:4362036

cx6

• Allelic Composition: Npy2r^tm1.1Hhz/Npy2r^tm1.1Hhz; Npy4r^tm1.1Hhz/Npy4r^tm1.1Hhz
• Genetic Background: involves: 129X1/SvJ * C57BL/6

homeostasis/metabolism

increased circulating insulin-like growth factor I level ( J:129609 )

• after fasting compared with similarly treated wild-type mice

increased growth hormone level ( J:129609 )

• pituitary gland cell growth hormone levels are increased in fasting mice compared to non-fasting mice and unlike in similarly treated wild-type mice

• pituitary gland cell growth hormone levels are increased in nonfasting mice compared with wild-type mice

abnormal testosterone level ( J:129609 )

• testosterone levels in Leydig cells are increased in fasting mice compared to in similarly treated wild-type mice