Phenotypes associated with this allele

• Allele Symbol: Tg(APP)8.9Btla
• Allele Name: transgene insertion 8.9, Bruce Lamb
• Allele ID: MGI:2447591
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Tg(APP)8.9Btla/Tg(APP)8.9Btla Tg(SOD1)51Yg/Tg(SOD1)51Yg	involves: 129S2/SvPas * BALB/c * C57BL/6J * DBA/2	MGI:3718511
cx2	Tg(APP)8.9Btla/Tg(APP)8.9Btla Tg(SOD1)69Yg/Tg(SOD1)69Yg	involves: 129S2/SvPas * BALB/c * C57BL/6J * DBA/2	MGI:3718514
cx3	Psen1^tm1.1Ruvi/Psen1^tm1.1Ruvi Tg(APP)8.9Btla/0	involves: 129S2/SvPas * C57BL/6J	MGI:5439825
tg4	Tg(APP)8.9Btla/Tg(APP)8.9Btla	involves: 129S2/SvPas	MGI:3718515

Genotype
MGI:3718511

cx1

• Allelic Composition: Tg(APP)8.9Btla/Tg(APP)8.9Btla; Tg(SOD1)51Yg/Tg(SOD1)51Yg
• Genetic Background: involves: 129S2/SvPas * BALB/c * C57BL/6J * DBA/2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

abnormal learning/memory/conditioning ( J:96742 )

• 9-month old mice show impaired memory retention in Morris water maze, exhibiting delayed escape latency compared to controls in all 6 trials

impaired short-term object recognition memory ( J:96742 )

• impairment observed at 10 months of age; mice show greater impairment than Tg(APP)8.9Btla mice

increased exploration in new environment ( J:96742 )

• exploratory behavior is increased for both familiar and novel objects

abnormal spatial learning ( J:96742 )

• at 9 months, a delay of learning process in Morris water maze is observed compared to controls; double mutants are significantly impaired relative to controls

• at 4.5 months of age, rate of learning platform position during first day of Morris water maze testing is impaired compared to controls or Tg(APP)8.9Btla/Tg(SOD1)69Yg double mutants

increased anxiety-related response ( J:96742 )

• mice spend greater amounts of time in the closed arms of an elevated plus maze than Tg(SOD1)51Yg or Tg(APP)8.9Btla mice; time per entry into dark arms is increased relative to Tg(APP)8.9Btla mice and total entries into dark arms is greater than controls

• mice spend much more time in dark side of dark-light box than either single transgenic line or controls

abnormal locomotor activation ( J:96742 )

• transgenic mice display more spontaneous locomotor activity than controls

nervous system

abnormal brain morphology ( J:96742 )

• mic show a 2-fold increase of cortical APP proteins and a 2.5-2.8-fold increase in hippocampal APP levels compared to Tg(APP)8.9Btla mice

• no Abeta peptides are detected in 5 month old mice

reduced long-term potentiation ( J:96742 )

• LTP is impaired

Genotype
MGI:3718514

cx2

• Allelic Composition: Tg(APP)8.9Btla/Tg(APP)8.9Btla; Tg(SOD1)69Yg/Tg(SOD1)69Yg
• Genetic Background: involves: 129S2/SvPas * BALB/c * C57BL/6J * DBA/2

behavior/neurological

abnormal learning/memory/conditioning ( J:96742 )

• 9-month old mice show impaired memory retention in Morris water maze, exhibiting delayed escape latency compared to controls in all 6 trials

impaired short-term object recognition memory ( J:96742 )

• impairment observed at 10 months of age in Morris water maze

abnormal spatial learning ( J:96742 )

• at 9 months, a delay of learning process in Morris water maze is observed compared to controls

• at 4.5 months of age, rate of learning platform position during first day of Morris water maze testing is impaired

nervous system

abnormal excitatory postsynaptic potential ( J:96742 )

• mice display depotentiation to a smaller EPSP size

reduced long-term potentiation ( J:96742 )

• LTP is impaired

homeostasis/metabolism

amyloidosis ( J:96742 )

• mic show a 1.6-fold increase of cortical APP proteins and a 1.8-2.1-fold increase in hippocampal APP levels compared to Tg(APP)8.9Btla mice

Genotype
MGI:5439825

cx3

• Allelic Composition: Psen1^tm1.1Ruvi/Psen1^tm1.1Ruvi; Tg(APP)8.9Btla/0
• Genetic Background: involves: 129S2/SvPas * C57BL/6J

nervous system

microgliosis ( J:187475 )

• keratan sulfate-positive-activated microglia

amyloid beta deposits ( J:187475 )

• scarce small plaques in the hippocampus of young animals becoming more prominent with age

• small and scattered plaques at 6 months in the cerebral cortex

• prominent plaques with age in the neocortex and leptomeninges

• small and moderate sized plaques in the cerebral cortex

• parenchymal amyloid deposition in the cerebellum of older mice

astrocytosis ( J:187475 )

• in the cerebral cortex with amyloid deposition

neurodegeneration ( J:187475 )

• neuritic dystrophy with clusters of swollen and abnormally distorted neuritic profiles

immune system

microgliosis ( J:187475 )

• keratan sulfate-positive-activated microglia

hematopoietic system

microgliosis ( J:187475 )

• keratan sulfate-positive-activated microglia

homeostasis/metabolism

amyloid beta deposits ( J:187475 )

• scarce small plaques in the hippocampus of young animals becoming more prominent with age

• small and scattered plaques at 6 months in the cerebral cortex

• prominent plaques with age in the neocortex and leptomeninges

• small and moderate sized plaques in the cerebral cortex

• parenchymal amyloid deposition in the cerebellum of older mice

Genotype
MGI:3718515

tg4

• Allelic Composition: Tg(APP)8.9Btla/Tg(APP)8.9Btla
• Genetic Background: involves: 129S2/SvPas

behavior/neurological

impaired short-term object recognition memory ( J:96742 )

• at 5.5 months of age, mice do not appear to recall platform position from the previous day of training and show a lower learning rate over subsequent trials

• impairment observed at 10 months of age

increased exploration in new environment ( J:96742 )

• exploratory behavior is increased for both familiar and novel objects

abnormal spatial learning ( J:96742 )

• at 9 months, a delay of learning process in Morris water maze is observed compared to controls

decreased anxiety-related response ( J:96742 )

• mice spend less time per entry into dark arms than controlsmice spend less time per entry into dark arms than controls

• mice exit dark side of dark-light box more often than controls

abnormal locomotor activation ( J:96742 )

• transgenic mice display more spontaneous locomotor activity than controls; activity is higher than in Tg(APP)8.9Btla or Tg(SOD1)51Yg mice

vision/eye

vision/eye phenotype ( J:80534 )

N • little or no perturbation of the retinas structure or cornea is observed

abnormal lens morphology ( J:80534 )

• numerous bodies with circular profiles are present in all lens sections examined

• circles are present in lenses from mice >6 months of age; circles are 1-5 um in diameter

• lenses from 6 and 16 month-old mice contain swollen cortical fiber cells and lens plaques

• fiber cell nuclei are disorganized in the outer cortical region compared to wild-type

• lens degeneration is variable in severity and onset; in some cases, one lens is more severely affected than the other side

abnormal lens fiber morphology ( J:80534 )

• individual fiber cells are irregularly shaped and fiber cell arrays are misaligned

• lens fiber cell membranes are morphologically abnormal

• mice display age-related fiber cell degeneration and cortical plaque formation

nervous system

nervous system phenotype ( J:96742 )

N • no Abeta peptides are detected in 5 month old mice

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
Alzheimer's disease		DOID:10652		J:96742