Phenotypes associated with this allele

• Allele Symbol: Hhex^tm1Ngu
• Allele Name: targeted mutation 1, Tamio Noguchi
• Allele ID: MGI:2448661
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Hhex^tm1Ngu/Hhex^tm1Ngu	involves: 129/Sv * C57BL/6	MGI:3628463

Genotype
MGI:3628463

hm1

• Allelic Composition: Hhex^tm1Ngu/Hhex^tm1Ngu
• Genetic Background: involves: 129/Sv * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:65105 )

• homozygotes die between E10.5 and E12.5

liver/biliary system

abnormal liver development ( J:65105 )

• at E9.5, homozygotes display absence of a typical liver bud; a thicker liver-like capsule and matrix-like structures are observed at E11.5, consisting of mesenchymal and undifferentiated endodermal cells

• at E10.5 and E11.5, the size of the liver-like structure is significantly reduced

• surprisingly, no histologic abnormalities are observed in the lung, thyroid or any other endoderm-derived tissue at E11.5; no malformation of neural development is noted

abnormal liver bud morphology ( J:65105 )

• at E9.5, homozygotes display absence of a typical liver bud

abnormal hepatocyte morphology ( J:65105 )

• although hematopoietic cells are found within the sinusoidal spaces, no parenchymal hepatocytes or apoptotic cells are histologically detected in the liver-like structure at E10.5 and E11.5

• at E8.5, mutant livers display absence of albumin expression; at E11.5, expression of several transcription factors involved in hepatocyte differentiation is reduced while expression of others is restricted to the capsule and internal matrix-like structures, indicating presence of undifferentiated endodermal cells

hematopoietic system

abnormal monocyte differentiation ( J:65105 )

• at E10.5, homozygotes show impaired hematopoetic differentiation of the monocyte lineage, with significantly reduced granulocytic-macrophage colony counts in isolated yolk sacs; in contrast, the erythroid lineage remains unaffected

impaired hematopoiesis ( J:65105 )

• at E11.5, homozygotes display impaired definitive hematopoiesis in liver due to arrested hepatogenesis, as shown by reduced beta-major globin mRNA levels

• however, definitive hematopoiesis is unaffected in other sites (aorta-gonad-mesonephros region and peripheral blood), as shown by histological and colony-formation assays

immune system

abnormal monocyte differentiation ( J:65105 )

• at E10.5, homozygotes show impaired hematopoetic differentiation of the monocyte lineage, with significantly reduced granulocytic-macrophage colony counts in isolated yolk sacs; in contrast, the erythroid lineage remains unaffected

cellular

abnormal monocyte differentiation ( J:65105 )

• at E10.5, homozygotes show impaired hematopoetic differentiation of the monocyte lineage, with significantly reduced granulocytic-macrophage colony counts in isolated yolk sacs; in contrast, the erythroid lineage remains unaffected