Phenotypes associated with this allele

• Allele Symbol: Tg(Lck-cre)1Cwi
• Allele Name: transgene insertion 1, Christopher B Wilson
• Allele ID: MGI:2448686
• Summary: 81 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Ptpn12^tm1Vei/Ptpn12^tm1Vei Tg(Lck-cre)1Cwi/0	B6.Cg-Ptpn12^tm1Vei Tg(Lck-cre)1Cwi	MGI:4830774
cn2	Ceacam1^tm1Rsb/Ceacam1^tm1Rsb Tg(Lck-cre)1Cwi/0	involves: 129	MGI:3695216
cn3	Smarca4^tm1Tich/Smarca4^+ Tg(Lck-cre)1Cwi/0	involves: 129	MGI:3830516
cn4	Smarca4^tm1.2Pcn/Smarca4^tm1Tich Tg(Lck-cre)1Cwi/0 Tg(LCKprBCL2L1)12Sjk/0	involves: 129	MGI:3830512
cn5	Smarca4^tm1.2Pcn/Smarca4^tm1Tich Tg(Lck-cre)1Cwi/0	involves: 129	MGI:3830511
cn6	Shc1^tm1Ravi/Shc1^tm1Ravi Tg(Lck-cre)1Cwi/?	involves: 129 * C57BL/6	MGI:3851535
cn7	Tg(EEF1A1-SHC1*)1Ravi/? Tg(Lck-cre)1Cwi/?	involves: 129 * C57BL/6	MGI:3851559
cn8	Sos1^tm1.1Les/Sos1^tm1.1Les Tg(Lck-cre)1Cwi/0	involves: 129 * C57BL/6	MGI:5141758
cn9	Caml^tm1Rjb/Caml^tm2Rjb Tg(Lck-cre)1Cwi/0	involves: 129 * C57BL/6 * DBA/2	MGI:3590220
cn10	Caml^tm1Rjb/Caml^tm2Rjb Tg(Lck-cre)1Cwi/0 Tg(TcraTcrb)1100Mjb/0	involves: 129 * C57BL/6 * DBA/2	MGI:3590221
cn11	Cbfb^tm1Itan/Cbfb^tm1Itan Tg(Lck-cre)1Cwi/0	involves: 129P2/OlaHsd	MGI:3761838
cn12	Runx1^tm1Tani/Runx1^tm1Tani Tg(Lck-cre)1Cwi/?	involves: 129P2/OlaHsd	MGI:3763097
cn13	Akt1^tm1Pnt/Akt1^tm1Pnt Akt2^tm1.1Mbb/Akt2^tm1.1Mbb Akt3^tm1Mbb/Akt3^tm1Mbb Tg(Lck-cre)1Cwi/0	involves: 129P2/OlaHsd	MGI:3803972
cn14	Akt1^tm1Pnt/Akt1^tm1Pnt Akt2^tm1.1Mbb/Akt2^tm1.1Mbb Tg(Lck-cre)1Cwi/0	involves: 129P2/OlaHsd	MGI:3803971
cn15	Fbxw7^tm1Kei/Fbxw7^tm1Kei Trp53^tm1Tyj/Trp53^tm1Tyj Tg(Lck-cre)1Cwi/?	involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6	MGI:3767597
cn16	Ctcf^tm2Gal/Ctcf^tm2Gal Rag2^tm1Fwa/Rag2^tm1Fwa Tg(Lck-cre)1Cwi/0	involves: 129P2/OlaHsd * 129S/SvEv * C57BL/6 * C57BL/10	MGI:3829023
cn17	Fbxw7^tm1Kei/Fbxw7^tm1Kei Tg(Lck-cre)1Cwi/?	involves: 129P2/OlaHsd * C57BL/6	MGI:3767595
cn18	Ctcf^tm2Gal/Ctcf^tm2Gal Tg(Lck-cre)1Cwi/0 Tg(TcraTcrb)425Cbn/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3829020
cn19	Ctcf^tm2Gal/Ctcf^+ Tg(Lck-cre)1Cwi/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3829017
cn20	Ctcf^tm2Gal/Ctcf^tm2Gal Tg(Lck-cre)1Cwi/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3829016
cn21	Cbfb^tm1Itan/Cbfb^tm1Itan Zbtb7b^tm2Litt/Zbtb7b^tm1.2Litt Tg(Lck-cre)1Cwi/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3821709
cn22	Cbfb^tm1Itan/Cbfb^tm1Itan Zbtb7b^tm2Litt/Zbtb7b^+ Tg(Lck-cre)1Cwi/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3821708
cn23	Zbtb7b^tm2Litt/Zbtb7b^tm1.2Litt Tg(Lck-cre)1Cwi/?	involves: 129P2/OlaHsd * C57BL/6	MGI:3821707
cn24	Ikzf1^tm1(Pax5)Mbu/Ikzf1^+ Tg(Lck-cre)1Cwi/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3701081
cn25	Ctcf^tm2Gal/Ctcf^tm2Gal Tg(Lck-cre)1Cwi/0 Tg(TcraH-Y,TcrbH-Y)71Vbo/0	involves: 129P2/OlaHsd * C57BL/6 * C57BL/10 * DBA/2J	MGI:3829022
cn26	Tnfsf11^tm1.1Htaka/Tnfsf11^tm1.2Htaka Tg(Lck-cre)1Cwi/0	involves: 129P2/OlaHsd * C57BL/6 * C57BL/6J	MGI:5297085
cn27	Myb^tm1Cgn/Myb^tm1Cgn Tg(Lck-cre)1Cwi/0	involves: 129P2/OlaHsd * C57BL/6 * DBA/2	MGI:3047333
cn28	Nkap^tm1.1Viss/Y Tg(Lck-cre)1Cwi/0	involves: 129S1/Sv * 129X1/SvJ	MGI:4839039
cn29	Vdac2^tm1.1Ehyc/Vdac2^tm1.1Ehyc Bak1^tm1Thsn/Bak1^tm1Thsn Tg(Lck-cre)1Cwi/?	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2	MGI:5691371
cn30	Bak1^tm1Thsn/Bak1^tm1Thsn Bax^tm2Sjk/Bax^tm2Sjk Tg(Lck-cre)1Cwi/?	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2	MGI:5691376
cn31	Igbp1^tm1Cbt/Igbp1^+ Tg(Lck-cre)1Cwi/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2	MGI:3056654
cn32	Igbp1^tm1Cbt/Y Tg(Lck-cre)1Cwi/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2	MGI:3056653
cn33	Chd4^tm1.1Kge/Chd4^tm1.2Kge Tg(CAG-Bgeo/GFP)21Lbe/0 Tg(Lck-cre)1Cwi/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL	MGI:3713384
cn34	Kmt2a^tm1.1Erns/Kmt2a^tm1.1Erns Tg(Lck-cre)1Cwi/0	involves: 129S2/SvPas * C57BL/6 * SJL	MGI:3839884
cn35	Foxo1^tm1.1Stlp/Foxo1^tm1.1Stlp Tg(Lck-cre)1Cwi/0	involves: 129S4/SvJae	MGI:4367754
cn36	Fadd^tm1Wnt/Fadd^tm1Wnt Tg(Fadd/EGFP)#Jizh/? Tg(Lck-cre)1Cwi/?	involves: 129S4/SvJae	MGI:4943254
cn37	Trp53^tm6Xu/Trp53^tm6Xu Tg(Lck-cre)1Cwi/0	involves: 129S4/SvJae	MGI:4430744
cn38	Tox^tm1Kay/Tox^tm1Kay Tg(Lck-cre)1Cwi/0	involves: 129S6/SvEvTac * C57BL/6	MGI:4839179
cn39	Was^tm1Sbs/Was^tm1Sbs Wasl^tm2Sbs/Wasl^tm2Sbs Tg(Lck-cre)1Cwi/?	involves: 129S6/SvEvTac * C57BL/6	MGI:3760284
cn40	Ptpmt1^tm2.1Ckq/Ptpmt1^tm2.1Ckq Tg(Lck-cre)1Cwi/0	involves: 129S6/SvEvTac * C57BL/6 * C57BL/6J * SJL	MGI:5485995
cn41	Ppp3r1^tm2Grc/Ppp3r1^tm2Grc Tg(Lck-cre)1Cwi/0	involves: 129S6/SvEvTac * C57BL/6 * DBA/2	MGI:3044045
cn42	Apc^tm1Kk/Apc^tm1Kk Tg(Lck-cre)1Cwi/?	involves: 129S6/SvEvTac * C57BL/6 * DBA/2	MGI:3590232
cn43	Ppp3r1^tm2Grc/Ppp3r1^tm2.1Grc Tg(Lck-cre)1Cwi/0	involves: 129S6/SvEvTac * C57BL/6 * DBA/2	MGI:3044044
cn44	Ptpn11^tm1Ckq/Ptpn11^+ Tg(Lck-cre)1Cwi/0	involves: 129S6/SvEvTac * C57BL/6J	MGI:5295469
cn45	Hdac1^tm1.1Shmc/Hdac1^tm1.1Shmc Hdac2^tm1.1Shmc/Hdac2^+ Tg(Lck-cre)1Cwi/0	involves: 129S7/SvEvBrd	MGI:5485404
cn46	Hdac1^tm1.1Shmc/Hdac1^tm1.1Shmc Hdac2^tm1.1Shmc/Hdac2^tm1.1Shmc Tg(Lck-cre)1Cwi/0	involves: 129S7/SvEvBrd	MGI:5485403
cn47	Hdac1^tm1.1Shmc/Hdac1^+ Hdac2^tm1.1Shmc/Hdac2^tm1.1Shmc Tg(Lck-cre)1Cwi/0	involves: 129S7/SvEvBrd	MGI:5485402
cn48	Hdac2^tm1.1Shmc/Hdac2^tm1.1Shmc Tg(Lck-cre)1Cwi/0	involves: 129S7/SvEvBrd	MGI:5485401
cn49	Hdac1^tm1.1Shmc/Hdac1^tm1.1Shmc Tg(Lck-cre)1Cwi/0	involves: 129S7/SvEvBrd	MGI:5485400
cn50	Trp53^tm1Brd/Trp53^tm1Brd Tg(Lck-cre)1Cwi/0	involves: 129S7/SvEvBrd	MGI:4430745
cn51	Smarca4^tm1.2Pcn/Smarca4^tm1Mag Tg(Lck-cre)1Cwi/?	involves: 129S/Sv * C57BL/6 * DBA/2	MGI:2677147
cn52	Smarca4^tm1.2Pcn/Smarca4^tm1Mag Tg(Lck-cre)1Cwi/? Tg(LCKprBCL2)36Sjk/?	involves: 129S/Sv * C57BL/6 * DBA/2	MGI:2677148
cn53	Rag2^tm1Fwa/Rag2^tm1Fwa Sos1^tm1.1Les/Sos1^tm1.1Les Tg(Lck-cre)1Cwi/0	involves: 129S/SvEv * C57BL/6	MGI:5141759
cn54	Birc5^tm1Wnt/Birc5^tm1Emc Tg(Lck-cre)1Cwi/0	involves: 129S/SvEv * C57BL/6 * DBA/2	MGI:3028491
cn55	Fbxw7^tm1Iaai/Fbxw7^tm1Iaai Tg(Lck-cre)1Cwi/0	involves: 129/Sv * C57BL/6	MGI:3802921
cn56	Mapk1^tm1Hed/Mapk1^tm1Hed Mapk3^tm1Gpg/Mapk3^tm1Gpg Tg(Lck-cre)1Cwi/0	involves: 129/Sv * C57BL/6 * DBA/2	MGI:3687234
cn57	Mcl1^tm2Sjk/Mcl1^tm3Sjk Tg(Lck-cre)1Cwi/0	involves: 129X1/SvJ * C57BL/6 * DBA/2	MGI:2684156
cn58	Vdac2^tm1.1Ehyc/Vdac2^tm1.1Ehyc Bax^tm2Sjk/Bax^tm2Sjk Tg(Lck-cre)1Cwi/?	involves: 129X1/SvJ * C57BL/6 * DBA/2	MGI:5691375
cn59	Vdac2^tm1.1Ehyc/Vdac2^tm1.1Ehyc Tg(Lck-cre)1Cwi/?	involves: 129X1/SvJ * C57BL/6 * DBA/2	MGI:5691369
cn60	Gata3^tm1Iho/Gata3^tm1Iho Tg(Lck-cre)1Cwi/0	involves: BALB/c	MGI:2683964
cn61	Gata3^tm1Iho/Gata3^tm1Iho Tg(DO11.10)10Dlo/0 Tg(Lck-cre)1Cwi/0	involves: BALB/c * C3H * C57BL/6	MGI:4417857
cn62	Prdm1^tm2.1Nutt/Prdm1^tm2.1Nutt Tg(Lck-cre)1Cwi/?	involves: C57BL/6	MGI:4355904
cn63	Sh2d1a^tm2Vei/Sh2d1a^tm2Vei Tg(Lck-cre)1Cwi/0	involves: C57BL/6	MGI:3775441
cn64	Taf7^tm1.1Dss/Taf7^tm1.2Dss Tg(Lck-cre)1Cwi/0	involves: C57BL/6	MGI:5430375
cn65	Hes1^tm1.1Frad/Hes1^tm1.1Frad Tg(Lck-cre)1Cwi/0	involves: C57BL/6	MGI:4868731
cn66	Mapk1^tm1.1Hed/Mapk1^tm1.1Hed Tg(Lck-cre)1Cwi/0	involves: C57BL/6 * DBA/2	MGI:3687233
cn67	Mapk1^tm1Hed/Mapk1^tm1Hed Tg(Lck-cre)1Cwi/0	involves: C57BL/6 * DBA/2	MGI:3687232
cn68	Shcbp1^tm1c(EUCOMM)Wtsi/Shcbp1^tm1c(EUCOMM)Wtsi Tg(Lck-cre)1Cwi/?	involves: C57BL/6 * DBA/2	MGI:5582921
cn69	Dicer1^tm1Mmk/Dicer1^tm1Mmk Tg(Lck-cre)1Cwi/0	involves: C57BL/6 * DBA/2	MGI:3578520
cn70	Nmt2^tm1.1Poru/Nmt2^tm1.1Poru Tg(Lck-cre)1Cwi/0	involves: C57BL/6 * DBA/2 * SJL	MGI:5696657
cn71	Mapk1^tm1Hed/Mapk1^tm1Hed Tg(Lck-cre)1Cwi/0 Tg(TcrAND)53Hed/0	involves: C57BL/6 * DBA/2 * SJL	MGI:3687239
cn72	Chd4^tm1.2Kge/Chd4^tm1.2Kge Tg(Lck-cre)1Cwi/0	involves: C57BL/6 * DBA/2 * SJL	MGI:3653117
cn73	Nmt1^tm1.1Poru/Nmt1^tm1.1Poru Nmt2^tm1.1Poru/Nmt2^tm1.1Poru Tg(Lck-cre)1Cwi/0	involves: C57BL/6 * DBA/2 * SJL	MGI:5696659
cn74	Nmt1^tm1.1Poru/Nmt1^tm1.1Poru Tg(Lck-cre)1Cwi/0	involves: C57BL/6 * DBA/2 * SJL	MGI:5696656
cn75	Chd4^tm1.1Kge/Chd4^tm1.2Kge Tg(Lck-cre)1Cwi/0	involves: C57BL/6 * SJL	MGI:3713382
cn76	Chd4^tm1.1Kge/Chd4^tm1.1Kge Tg(Lck-cre)1Cwi/0	involves: C57BL/6 * SJL	MGI:3713383
cn77	Akt1^tm1Pnt/Akt1^tm1Pnt Akt3^tm1Mbb/Akt3^tm1Mbb Tg(Lck-cre)1Cwi/0	Not Specified	MGI:3803970
cn78	Akt1^tm1Pnt/Akt1^tm1Pnt Tg(Lck-cre)1Cwi/0	Not Specified	MGI:3803966
cn79	Smarca4^tm1.2Pcn/Smarca4^tm1.2Pcn Tg(Lck-cre)1Cwi/0 Tg(LCKprBCL2L1)12Sjk/0	Not Specified	MGI:3830514
cn80	Smarca4^tm1.2Pcn/Smarca4^tm1.2Pcn Tg(Lck-cre)1Cwi/0	Not Specified	MGI:3830513
tg81	Tg(Lck-cre)1Cwi/0	involves: C57BL/6 * DBA/2	MGI:5696658

Genotype
MGI:4830774

cn1

• Allelic Composition: Ptpn12^tm1Vei/Ptpn12^tm1Vei; Tg(Lck-cre)1Cwi/0
• Genetic Background: B6.Cg-Ptpn12^tm1Vei Tg(Lck-cre)1Cwi

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased susceptibility to experimental autoimmune encephalomyelitis ( J:163910 )

• much less susceptible to experimental autoimmune encephalitis induced by antibodies to Mog

• primary immune response to Mog is normal

Genotype
MGI:3695216

cn2

• Allelic Composition: Ceacam1^tm1Rsb/Ceacam1^tm1Rsb; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129

immune system

increased T cell proliferation ( J:116136 )

• anti-CD3 and anti-CD28 stimulation enhances proliferation compared to wild-type

abnormal cytokine secretion ( J:116136 )

• anti-CD3 and anti-CD28 stimulation enhances cytokine production by T cells

increased interferon-gamma secretion ( J:116136 )

• Ifng secretion by T cells upon anti-CD3 and anti-CD28 stimulation is enhanced

increased interleukin-2 secretion ( J:116136 )

• IL-2 secretion by T cells upon anti-CD3 and anti-CD28 stimulation is enhanced

hematopoietic system

increased T cell proliferation ( J:116136 )

• anti-CD3 and anti-CD28 stimulation enhances proliferation compared to wild-type

cellular

increased T cell proliferation ( J:116136 )

• anti-CD3 and anti-CD28 stimulation enhances proliferation compared to wild-type

Genotype
MGI:3830516

cn3

• Allelic Composition: Smarca4^tm1Tich/Smarca4⁺; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129

immune system

decreased thymocyte number ( J:144072 )

• total thymocyte numbers are slightly reduced compared to in wild-type mice

decreased double-negative T cell number ( J:144072 )

• DN4 cells are completely missing

decreased double-positive T cell number ( J:144072 )

decreased single-positive T cell number ( J:144072 )

• immature single positive cells are lost

hematopoietic system

decreased thymocyte number ( J:144072 )

• total thymocyte numbers are slightly reduced compared to in wild-type mice

decreased double-negative T cell number ( J:144072 )

• DN4 cells are completely missing

decreased double-positive T cell number ( J:144072 )

decreased single-positive T cell number ( J:144072 )

• immature single positive cells are lost

endocrine/exocrine glands

decreased thymocyte number ( J:144072 )

• total thymocyte numbers are slightly reduced compared to in wild-type mice

Genotype
MGI:3830512

cn4

• Allelic Composition: Smarca4^tm1.2Pcn/Smarca4^tm1Tich; Tg(Lck-cre)1Cwi/0; Tg(LCKprBCL2L1)12Sjk/0
• Genetic Background: involves: 129

immune system

abnormal T cell morphology ( J:144072 )

• unlike in Smarca4^tm1Grc/Smarca4^tm1Grc Tg(Lck-cre)1Cwi mice, a CD4-CD8+ population is not observed

• 25% of the post-DN3 population is composed of CD4-DN4 cells compared to 9% in Smarca4^tm1Grc/Smarca4^tm1Grc Tg(Lck-cre)1Cwi Tg(LCKprBCL2L1)12Sjk mice

• 39% of the post-DN3 population is composed of CD4+DN4 cells compared to 52% in Smarca4^tm1Grc/Smarca4^tm1Grc Tg(Lck-cre)1Cwi Tg(LCKprBCL2L1)12Sjk mice

• mice exhibit two CD4+ populations with different levels of CD4 expression

increased thymocyte number ( J:144072 )

increased double-negative T cell number ( J:144072 )

decreased double-positive T cell number ( J:144072 )

hematopoietic system

abnormal T cell morphology ( J:144072 )

• mice exhibit two CD4+ populations with different levels of CD4 expression

• unlike in Smarca4^tm1Grc/Smarca4^tm1Grc Tg(Lck-cre)1Cwi mice, a CD4-CD8+ population is not observed

• 25% of the post-DN3 population is composed of CD4-DN4 cells compared to 9% in Smarca4^tm1Grc/Smarca4^tm1Grc Tg(Lck-cre)1Cwi Tg(LCKprBCL2L1)12Sjk mice

• 39% of the post-DN3 population is composed of CD4+DN4 cells compared to 52% in Smarca4^tm1Grc/Smarca4^tm1Grc Tg(Lck-cre)1Cwi Tg(LCKprBCL2L1)12Sjk mice

increased thymocyte number ( J:144072 )

increased double-negative T cell number ( J:144072 )

decreased double-positive T cell number ( J:144072 )

endocrine/exocrine glands

increased thymocyte number ( J:144072 )

Genotype
MGI:3830511

cn5

• Allelic Composition: Smarca4^tm1.2Pcn/Smarca4^tm1Tich; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129

immune system

abnormal T cell morphology ( J:144072 )

• 38% of the post-DN3 population is composed of CD4+CD8- cells compared to 14% in Smarca4^tm1Grc/Smarca4^tm1Grc Tg(Lck-cre)1Cwi mice

• unlike in Smarca4^tm1Grc/Smarca4^tm1Grc Tg(Lck-cre)1Cwi mice, a CD4-CD8+ population analogous to immature single positive cells is not observed

decreased thymocyte number ( J:144072 )

• thymocyte numbers are reduced 13-fold compared to in wild-type mice

decreased double-negative T cell number ( J:144072 )

• post-DN3 T cells are reduced compared to in wild-type mice

• however, DN3 cellularity is normal

hematopoietic system

abnormal T cell morphology ( J:144072 )

• 38% of the post-DN3 population is composed of CD4+CD8- cells compared to 14% in Smarca4^tm1Grc/Smarca4^tm1Grc Tg(Lck-cre)1Cwi mice

• unlike in Smarca4^tm1Grc/Smarca4^tm1Grc Tg(Lck-cre)1Cwi mice, a CD4-CD8+ population analogous to immature single positive cells is not observed

decreased thymocyte number ( J:144072 )

• thymocyte numbers are reduced 13-fold compared to in wild-type mice

decreased double-negative T cell number ( J:144072 )

• post-DN3 T cells are reduced compared to in wild-type mice

• however, DN3 cellularity is normal

endocrine/exocrine glands

decreased thymocyte number ( J:144072 )

• thymocyte numbers are reduced 13-fold compared to in wild-type mice

Genotype
MGI:3851535

cn6

• Allelic Composition: Shc1^tm1Ravi/Shc1^tm1Ravi; Tg(Lck-cre)1Cwi/?
• Genetic Background: involves: 129 * C57BL/6

immune system

thymus hypoplasia ( J:78180 )

• thymus cellularity is only 10-30% of controls

abnormal double-negative T cell morphology ( J:78180 )

• double-negative thymocyte numbers are similar to wild-type but the distribution of the sub-populations differs

• these DN3 cells have less proliferation suggesting a partial block in development

decreased double-positive T cell number ( J:78180 )

• double-positive thymocyte numbers are decreased compared to wild-type while double-negative numbers are close to normal

decreased single-positive T cell number ( J:78180 )

• single-positive thymocyte numbers are decreased compared to controls

hematopoietic system

thymus hypoplasia ( J:78180 )

• thymus cellularity is only 10-30% of controls

abnormal double-negative T cell morphology ( J:78180 )

• double-negative thymocyte numbers are similar to wild-type but the distribution of the sub-populations differs

• these DN3 cells have less proliferation suggesting a partial block in development

decreased double-positive T cell number ( J:78180 )

• double-positive thymocyte numbers are decreased compared to wild-type while double-negative numbers are close to normal

decreased single-positive T cell number ( J:78180 )

• single-positive thymocyte numbers are decreased compared to controls

endocrine/exocrine glands

thymus hypoplasia ( J:78180 )

• thymus cellularity is only 10-30% of controls

Genotype
MGI:3851559

cn7

• Allelic Composition: Tg(EEF1A1-SHC1*)1Ravi/?; Tg(Lck-cre)1Cwi/?
• Genetic Background: involves: 129 * C57BL/6

immune system

thymus hypoplasia ( J:78180 )

• thymus cellularity is only 10-30% of controls

abnormal double-negative T cell morphology ( J:78180 )

• double-negative thymocyte numbers are similar to wild-type but the distribution of the sub-populations differs

• mice have a 83% DN3 stage thymocytes compared to 48% in normal mice, with a concomitant decrease in the DN4 stage thymocytes (46% in normal versus 8% in double-transgenic mice)

• these DN3 cells have less proliferation suggesting a partial block in development

decreased double-positive T cell number ( J:78180 )

• double-positive thymocyte numbers are decreased compared to wild-type while double-negative numbers are close to normal

decreased single-positive T cell number ( J:78180 )

• single-positive thymocyte numbers are decreased compared to controls

hematopoietic system

thymus hypoplasia ( J:78180 )

• thymus cellularity is only 10-30% of controls

abnormal double-negative T cell morphology ( J:78180 )

• double-negative thymocyte numbers are similar to wild-type but the distribution of the sub-populations differs

• mice have a 83% DN3 stage thymocytes compared to 48% in normal mice, with a concomitant decrease in the DN4 stage thymocytes (46% in normal versus 8% in double-transgenic mice)

• these DN3 cells have less proliferation suggesting a partial block in development

decreased double-positive T cell number ( J:78180 )

• double-positive thymocyte numbers are decreased compared to wild-type while double-negative numbers are close to normal

decreased single-positive T cell number ( J:78180 )

• single-positive thymocyte numbers are decreased compared to controls

endocrine/exocrine glands

thymus hypoplasia ( J:78180 )

• thymus cellularity is only 10-30% of controls

Genotype
MGI:5141758

cn8

• Allelic Composition: Sos1^tm1.1Les/Sos1^tm1.1Les; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129 * C57BL/6

immune system

decreased T cell proliferation ( J:174536 )

• a 25% reduction in proliferation of both DN4 and DP thymocytes

decreased thymocyte number ( J:174536 )

• 50% reduction in total thymocyte numbers

decreased DN4 thymocyte number ( J:174536 )

• decrease in the number and percentage of DN4 cells and an increase in the DN3:DN4 ratio

decreased double-negative T cell number ( J:174536 )

• while the total number is reduced the percentage of DN cells is increased

decreased double-positive T cell number ( J:174536 )

decreased CD4-positive, alpha-beta T cell number ( J:174536 )

• decrease in the number of CD4+ thymocytes

• however, the number of peripheral CD4+ lymph node T cells is not decreased

decreased CD8-positive, alpha-beta T cell number ( J:174536 )

• decrease in the number of CD8+ thymocytes

• however, the number of peripheral CD8+ lymph node T cells is not decreased

decreased gamma-delta T cell number ( J:174536 )

• decreased by 50%

hematopoietic system

decreased T cell proliferation ( J:174536 )

• a 25% reduction in proliferation of both DN4 and DP thymocytes

decreased thymocyte number ( J:174536 )

• 50% reduction in total thymocyte numbers

decreased DN4 thymocyte number ( J:174536 )

• decrease in the number and percentage of DN4 cells and an increase in the DN3:DN4 ratio

decreased double-negative T cell number ( J:174536 )

• while the total number is reduced the percentage of DN cells is increased

decreased double-positive T cell number ( J:174536 )

decreased CD4-positive, alpha-beta T cell number ( J:174536 )

• decrease in the number of CD4+ thymocytes

• however, the number of peripheral CD4+ lymph node T cells is not decreased

decreased CD8-positive, alpha-beta T cell number ( J:174536 )

• decrease in the number of CD8+ thymocytes

• however, the number of peripheral CD8+ lymph node T cells is not decreased

decreased gamma-delta T cell number ( J:174536 )

• decreased by 50%

endocrine/exocrine glands

decreased thymocyte number ( J:174536 )

• 50% reduction in total thymocyte numbers

decreased DN4 thymocyte number ( J:174536 )

• decrease in the number and percentage of DN4 cells and an increase in the DN3:DN4 ratio

cellular

decreased T cell proliferation ( J:174536 )

• a 25% reduction in proliferation of both DN4 and DP thymocytes

Genotype
MGI:3590220

cn9

• Allelic Composition: Caml^tm1Rjb/Caml^tm2Rjb; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129 * C57BL/6 * DBA/2

hematopoietic system

increased T cell apoptosis ( J:100536 )

• double-positive thymocytes from mutant mice undergo enhanced apoptosis by TCR-stimulation

decreased thymocyte number ( J:100536 )

• the average numbers of total thymocytes were reduced by half

• the number of cells at the double-negative stage was not different

decreased single-positive T cell number ( J:100536 )

• 4- to 6-fold fewer CD4 and CD8 single-positive cells compared to control

• in 3-4 weeks old mutant mice, there were 7- to 9-fold fewer peripheral CD4+ and CD8+ T cells

• in 8-9 weeks old mutant mice, T cell numbers were more similar

immune system

increased T cell apoptosis ( J:100536 )

• double-positive thymocytes from mutant mice undergo enhanced apoptosis by TCR-stimulation

decreased thymocyte number ( J:100536 )

• the average numbers of total thymocytes were reduced by half

• the number of cells at the double-negative stage was not different

decreased single-positive T cell number ( J:100536 )

• 4- to 6-fold fewer CD4 and CD8 single-positive cells compared to control

• in 3-4 weeks old mutant mice, there were 7- to 9-fold fewer peripheral CD4+ and CD8+ T cells

• in 8-9 weeks old mutant mice, T cell numbers were more similar

cellular

increased T cell apoptosis ( J:100536 )

• double-positive thymocytes from mutant mice undergo enhanced apoptosis by TCR-stimulation

endocrine/exocrine glands

decreased thymocyte number ( J:100536 )

• the average numbers of total thymocytes were reduced by half

• the number of cells at the double-negative stage was not different

Genotype
MGI:3590221

cn10

• Allelic Composition: Caml^tm1Rjb/Caml^tm2Rjb; Tg(Lck-cre)1Cwi/0; Tg(TcraTcrb)1100Mjb/0
• Genetic Background: involves: 129 * C57BL/6 * DBA/2

hematopoietic system

decreased thymocyte number ( J:100536 )

• reduced numbers of total thymocytes

abnormal negative T cell selection ( J:100536 )

• in an in vitro assay, mutant thymocytes underwent OCA peptide 257-264 dependent cell death more readily than control, indicating enhanced negative selection

abnormal positive T cell selection ( J:100536 )

• lower proportions of double-positive and single-positive cells, and reduced CD8 SP cell in the peripheral T cell population, both indicating impaired positive selection

immune system

decreased thymocyte number ( J:100536 )

• reduced numbers of total thymocytes

abnormal negative T cell selection ( J:100536 )

• in an in vitro assay, mutant thymocytes underwent OCA peptide 257-264 dependent cell death more readily than control, indicating enhanced negative selection

abnormal positive T cell selection ( J:100536 )

• lower proportions of double-positive and single-positive cells, and reduced CD8 SP cell in the peripheral T cell population, both indicating impaired positive selection

endocrine/exocrine glands

decreased thymocyte number ( J:100536 )

• reduced numbers of total thymocytes

Genotype
MGI:3761838

cn11

• Allelic Composition: Cbfb^tm1Itan/Cbfb^tm1Itan; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129P2/OlaHsd

immune system

decreased thymocyte number ( J:125953 )

abnormal CD4-positive T cell differentiation ( J:125953 )

• 2 fold decrease in total number

abnormal T-helper 1 cell differentiation ( J:125953 )

• slightly increased levels of the Th-2 cytokine IL-4 are produced when nave T cells are activated in vitro

• a small subset of T cells produce both IL-4 and IFN-gamma when nave T cells are cultured under Th1 polarizing conditions

abnormal CD8-positive, alpha-beta T cell differentiation ( J:125953 )

• very few CD8-positive T cells are found in the thymus

abnormal double-positive T cell morphology ( J:125953 )

• increase in DP T cell due to reduced ability of T cell precursors to mature past double positive stage

hematopoietic system

decreased thymocyte number ( J:125953 )

abnormal CD4-positive T cell differentiation ( J:125953 )

• 2 fold decrease in total number

abnormal T-helper 1 cell differentiation ( J:125953 )

• slightly increased levels of the Th-2 cytokine IL-4 are produced when nave T cells are activated in vitro

• a small subset of T cells produce both IL-4 and IFN-gamma when nave T cells are cultured under Th1 polarizing conditions

abnormal CD8-positive, alpha-beta T cell differentiation ( J:125953 )

• very few CD8-positive T cells are found in the thymus

abnormal double-positive T cell morphology ( J:125953 )

• increase in DP T cell due to reduced ability of T cell precursors to mature past double positive stage

endocrine/exocrine glands

decreased thymocyte number ( J:125953 )

Genotype
MGI:3763097

cn12

• Allelic Composition: Runx1^tm1Tani/Runx1^tm1Tani; Tg(Lck-cre)1Cwi/?
• Genetic Background: involves: 129P2/OlaHsd

immune system

decreased thymocyte number ( J:125959 )

• six fold less cells as a result of arrested T cell development

increased double-negative T cell number ( J:125959 )

• developing T cells fail to make the transition between DN3 to DN4 stag

decreased double-positive T cell number ( J:125959 )

• resulting from arrested T cell development

arrested T cell differentiation ( J:125959 )

• developing T cells fail to make the transition between DN3 to DN4 stage

hematopoietic system

decreased thymocyte number ( J:125959 )

• six fold less cells as a result of arrested T cell development

increased double-negative T cell number ( J:125959 )

• developing T cells fail to make the transition between DN3 to DN4 stag

decreased double-positive T cell number ( J:125959 )

• resulting from arrested T cell development

arrested T cell differentiation ( J:125959 )

• developing T cells fail to make the transition between DN3 to DN4 stage

endocrine/exocrine glands

decreased thymocyte number ( J:125959 )

• six fold less cells as a result of arrested T cell development

Genotype
MGI:3803972

cn13

• Allelic Composition: Akt1^tm1Pnt/Akt1^tm1Pnt; Akt2^tm1.1Mbb/Akt2^tm1.1Mbb; Akt3^tm1Mbb/Akt3^tm1Mbb; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129P2/OlaHsd

hematopoietic system

increased T cell apoptosis ( J:135903 )

• double negative thymocytes of all stages have higher rates of apoptosis

thymus hypoplasia ( J:135903 )

• thymus cellularity is reduced by about 20-fold

increased double-negative T cell number ( J:135903 )

• the percentage of double negative T cells in the thymus is 85.4% compared to 2.7% in wild-type mice

• there is a significant reduction in proliferating DN4 cells suggesting a partial development block in transitioning to the DP stage

decreased double-positive T cell number ( J:135903 )

• the percentage of double positive T cells in the thymus is reduced to 1.8% compared to 84.6% in wild-type mice

• the actual number of DP T cells is 50-fold less than is found in wild-type thymuses

arrested T cell differentiation ( J:135903 )

• developing thymocytes are almost completely blocked at the DN4 to DP transition

immune system

increased T cell apoptosis ( J:135903 )

• double negative thymocytes of all stages have higher rates of apoptosis

thymus hypoplasia ( J:135903 )

• thymus cellularity is reduced by about 20-fold

increased double-negative T cell number ( J:135903 )

• the percentage of double negative T cells in the thymus is 85.4% compared to 2.7% in wild-type mice

• there is a significant reduction in proliferating DN4 cells suggesting a partial development block in transitioning to the DP stage

decreased double-positive T cell number ( J:135903 )

• the percentage of double positive T cells in the thymus is reduced to 1.8% compared to 84.6% in wild-type mice

• the actual number of DP T cells is 50-fold less than is found in wild-type thymuses

arrested T cell differentiation ( J:135903 )

• developing thymocytes are almost completely blocked at the DN4 to DP transition

cellular

increased T cell apoptosis ( J:135903 )

• double negative thymocytes of all stages have higher rates of apoptosis

endocrine/exocrine glands

thymus hypoplasia ( J:135903 )

• thymus cellularity is reduced by about 20-fold

Genotype
MGI:3803971

cn14

• Allelic Composition: Akt1^tm1Pnt/Akt1^tm1Pnt; Akt2^tm1.1Mbb/Akt2^tm1.1Mbb; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129P2/OlaHsd

immune system

increased T cell apoptosis ( J:135903 )

• an increased percentage of double positive thymocytes are apoptotic (15.9% compared to 2.6%)

thymus hypoplasia ( J:135903 )

• thymus cellularity is reduced by about 15-fold

increased double-negative T cell number ( J:135903 )

• the percentage of double negative T cells in the thymus is 56.2% compared to 2.7% in wild-type mice

• there is a significant reduction in proliferating DN4 cells suggesting a partial development block in transitioning to the DP stage

decreased double-positive T cell number ( J:135903 )

• the percentage of double positive T cells in the thymus is reduced to 25% compared to 84.6% in wild-type mice

• the actual number of DP T cells is 50-fold less than is found in wild-type thymuses

arrested T cell differentiation ( J:135903 )

• developing thymocytes are partially blocked at the DN3 to DN4 stage and the DN4 to the DP stage

hematopoietic system

increased T cell apoptosis ( J:135903 )

• an increased percentage of double positive thymocytes are apoptotic (15.9% compared to 2.6%)

thymus hypoplasia ( J:135903 )

• thymus cellularity is reduced by about 15-fold

increased double-negative T cell number ( J:135903 )

• the percentage of double negative T cells in the thymus is 56.2% compared to 2.7% in wild-type mice

• there is a significant reduction in proliferating DN4 cells suggesting a partial development block in transitioning to the DP stage

decreased double-positive T cell number ( J:135903 )

• the percentage of double positive T cells in the thymus is reduced to 25% compared to 84.6% in wild-type mice

• the actual number of DP T cells is 50-fold less than is found in wild-type thymuses

arrested T cell differentiation ( J:135903 )

• developing thymocytes are partially blocked at the DN3 to DN4 stage and the DN4 to the DP stage

cellular

increased T cell apoptosis ( J:135903 )

• an increased percentage of double positive thymocytes are apoptotic (15.9% compared to 2.6%)

endocrine/exocrine glands

thymus hypoplasia ( J:135903 )

• thymus cellularity is reduced by about 15-fold

Genotype
MGI:3767597

cn15

• Allelic Composition: Fbxw7^tm1Kei/Fbxw7^tm1Kei; Trp53^tm1Tyj/Trp53^tm1Tyj; Tg(Lck-cre)1Cwi/?
• Genetic Background: involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6

neoplasm

increased lymphoma incidence ( J:128523 )

• mice develop double positive lymphomas at increased frequency and decreased latency compared to Fbxw7^tm1Kei/Fbxw7^tm1Kei Tg(Lck-cre)1Cwi mice

increased T cell derived lymphoma incidence ( J:128523 )

• at 8 weeks of age 2 mice develop thymic lymphoma

endocrine/exocrine glands

increased T cell derived lymphoma incidence ( J:128523 )

• at 8 weeks of age 2 mice develop thymic lymphoma

immune system

immune system phenotype ( J:128523 )

N • unlike in Fbxw7^tm1Kei/Fbxw7^tm1Kei Tg(Lck-cre)1Cwi mice, T cells do not arrest in S phase or undergo increased apoptosis

increased T cell derived lymphoma incidence ( J:128523 )

• at 8 weeks of age 2 mice develop thymic lymphoma

hematopoietic system

increased T cell derived lymphoma incidence ( J:128523 )

• at 8 weeks of age 2 mice develop thymic lymphoma

Genotype
MGI:3829023

cn16

• Allelic Composition: Ctcf^tm2Gal/Ctcf^tm2Gal; Rag2^tm1Fwa/Rag2^tm1Fwa; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129P2/OlaHsd * 129S/SvEv * C57BL/6 * C57BL/10

immune system

decreased double-positive T cell number ( J:143271 )

• despite increased double positive T cell production stimulated by anti-CD3epsilon treatment, mice produce fewer double positive T cells compared to similarly treated Rag2^tm1Fwa homozygotes

hematopoietic system

decreased double-positive T cell number ( J:143271 )

• despite increased double positive T cell production stimulated by anti-CD3epsilon treatment, mice produce fewer double positive T cells compared to similarly treated Rag2^tm1Fwa homozygotes

Genotype
MGI:3767595

cn17

• Allelic Composition: Fbxw7^tm1Kei/Fbxw7^tm1Kei; Tg(Lck-cre)1Cwi/?
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

mortality/aging

premature death ( J:128523 )

• mice that develop lymphomas die between 14 and 60 weeks

immune system

increased T cell apoptosis ( J:128523 )

• after stimulation with antigen, T cell progression into S phase is inhibited and apoptosis is increased

enlarged thymus ( J:128523 )

increased double-positive T cell number ( J:128523 )

• the number and percent of double positive thymocytes is increased compared to in wild-type mice due to increased proliferation of double positive thymocytes

• however, the number and proliferation of double negative, CD4+ single positive and CD8+ single positive thymocytes are normal

arrested T cell differentiation ( J:128523 )

• the number of single positive T cells is decreased in peripheral organs such as the spleen and lymph nodes

decreased single-positive T cell number ( J:128523 )

• the number of single positive T cells is decreased in peripheral organs such as the spleen and lymph nodes

abnormal T cell activation ( J:128523 )

• T cells fail to proliferate in response to anti-CD3 or the combination of phorbol 12,13-dibutyrate and ionomycin

• after stimulation with antigen, T cell progression into S phase is inhibited and apoptosis is increased

neoplasm

increased lymphoma incidence ( J:128523 )

• 12 of 23 mice develop aggressive lymphomas with massive thymic enlargement due to clonal expansion of double positive thymocytes

• tumors contain uniform populations of immature lymphoid cells with some areas of necrosis

• early and late onset tumors exhibit identical phenotype

hematopoietic system

increased T cell apoptosis ( J:128523 )

• after stimulation with antigen, T cell progression into S phase is inhibited and apoptosis is increased

enlarged thymus ( J:128523 )

increased double-positive T cell number ( J:128523 )

• the number and percent of double positive thymocytes is increased compared to in wild-type mice due to increased proliferation of double positive thymocytes

• however, the number and proliferation of double negative, CD4+ single positive and CD8+ single positive thymocytes are normal

arrested T cell differentiation ( J:128523 )

• the number of single positive T cells is decreased in peripheral organs such as the spleen and lymph nodes

decreased single-positive T cell number ( J:128523 )

• the number of single positive T cells is decreased in peripheral organs such as the spleen and lymph nodes

abnormal T cell activation ( J:128523 )

• T cells fail to proliferate in response to anti-CD3 or the combination of phorbol 12,13-dibutyrate and ionomycin

• after stimulation with antigen, T cell progression into S phase is inhibited and apoptosis is increased

cellular

increased T cell apoptosis ( J:128523 )

• after stimulation with antigen, T cell progression into S phase is inhibited and apoptosis is increased

endocrine/exocrine glands

enlarged thymus ( J:128523 )

Genotype
MGI:3829020

cn18

• Allelic Composition: Ctcf^tm2Gal/Ctcf^tm2Gal; Tg(Lck-cre)1Cwi/0; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

abnormal T cell differentiation ( J:143271 )

• T cell development is more severely arrested than in Ctcf^tm1Laat/Ctcf^tm1Laat Tg(Lck-cre)1Cwi mice

increased double-negative T cell number ( J:143271 )

decreased double-positive T cell number ( J:143271 )

decreased T cell number ( J:143271 )

• mice exhibit an almost complete lack of mature T cells in the spleen

decreased CD4-positive, alpha-beta T cell number ( J:143271 )

increased single-positive T cell number ( J:143271 )

• the number of immature single positive cells in the thymus is increased compared to in wild-type mice

hematopoietic system

abnormal T cell differentiation ( J:143271 )

• T cell development is more severely arrested than in Ctcf^tm1Laat/Ctcf^tm1Laat Tg(Lck-cre)1Cwi mice

increased double-negative T cell number ( J:143271 )

decreased T cell number ( J:143271 )

• mice exhibit an almost complete lack of mature T cells in the spleen

decreased double-positive T cell number ( J:143271 )

decreased CD4-positive, alpha-beta T cell number ( J:143271 )

increased single-positive T cell number ( J:143271 )

• the number of immature single positive cells in the thymus is increased compared to in wild-type mice

Genotype
MGI:3829017

cn19

• Allelic Composition: Ctcf^tm2Gal/Ctcf⁺; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

thymus hypoplasia ( J:143271 )

• modestly

decreased CD4-positive, alpha-beta T cell number ( J:143271 )

• the numbers of mature CD4+ and CD8+ cells in the spleen and lymph nodes are reduced compared to in wild-type mice

• however, the ratio of mature single positive cells to double positive cells is normal

decreased CD8-positive, alpha-beta T cell number ( J:143271 )

• the numbers of mature CD4+ and CD8+ cells in the spleen and lymph nodes are reduced compared to in wild-type mice

• however, the ratio of mature single positive cells to double positive cells is normal

hematopoietic system

thymus hypoplasia ( J:143271 )

• modestly

decreased CD4-positive, alpha-beta T cell number ( J:143271 )

• the numbers of mature CD4+ and CD8+ cells in the spleen and lymph nodes are reduced compared to in wild-type mice

• however, the ratio of mature single positive cells to double positive cells is normal

decreased CD8-positive, alpha-beta T cell number ( J:143271 )

• the numbers of mature CD4+ and CD8+ cells in the spleen and lymph nodes are reduced compared to in wild-type mice

• however, the ratio of mature single positive cells to double positive cells is normal

endocrine/exocrine glands

thymus hypoplasia ( J:143271 )

• modestly

Genotype
MGI:3829016

cn20

• Allelic Composition: Ctcf^tm2Gal/Ctcf^tm2Gal; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

thymus hypoplasia ( J:143271 )

abnormal T cell morphology ( J:143271 )

• cells at the DN3L to immature single positive stage are smaller than in wild-type mice while double positive cells are larger than in wild-type mice

abnormal T cell differentiation ( J:143271 )

• alpha beta T cell differentiation is defective

• the number of DN3, DN4 and immature single positive cells is increased compared to in wild-type mice

• mice exhibit only a 4-fold increase in thymocytes during the transition from immature single positive to double positive T cells compared to a 90-fold increase in wild-type mice

• however, sigma delta T cell differentiation and TCR rearrangement are normal

• mice exhibit a defect in the transition from immature single positive cells to double positive cells

• the number of immature single positive T cells undergoing cell cycling is half as many as in wild-type mice

increased double-negative T cell number ( J:143271 )

• the number of double negative DN3 and DN4 cells is increased compared to in wild-type mice

decreased double-positive T cell number ( J:143271 )

decreased CD4-positive, alpha-beta T cell number ( J:143271 )

• the numbers of mature CD4+ and CD8+ cells in the spleen and lymph nodes are reduced compared to in wild-type mice

• however, the ratio of mature single positive cells to double positive cells is normal

decreased CD8-positive, alpha-beta T cell number ( J:143271 )

• the numbers of mature CD4+ and CD8+ cells in the spleen and lymph nodes are reduced compared to in wild-type mice

• however, the ratio of mature single positive cells to double positive cells is normal

decreased single-positive T cell number ( J:143271 )

• the number of mature single positive T cells is decreased compared to in wild-type mice

increased single-positive T cell number ( J:143271 )

• the number of immature single positive cells in the thymus is increased compared to in wild-type mice

hematopoietic system

thymus hypoplasia ( J:143271 )

abnormal T cell morphology ( J:143271 )

• cells at the DN3L to immature single positive stage are smaller than in wild-type mice while double positive cells are larger than in wild-type mice

abnormal T cell differentiation ( J:143271 )

• alpha beta T cell differentiation is defective

• the number of DN3, DN4 and immature single positive cells is increased compared to in wild-type mice

• mice exhibit only a 4-fold increase in thymocytes during the transition from immature single positive to double positive T cells compared to a 90-fold increase in wild-type mice

• however, sigma delta T cell differentiation and TCR rearrangement are normal

• mice exhibit a defect in the transition from immature single positive cells to double positive cells

• the number of immature single positive T cells undergoing cell cycling is half as many as in wild-type mice

increased double-negative T cell number ( J:143271 )

• the number of double negative DN3 and DN4 cells is increased compared to in wild-type mice

decreased double-positive T cell number ( J:143271 )

decreased CD4-positive, alpha-beta T cell number ( J:143271 )

• the numbers of mature CD4+ and CD8+ cells in the spleen and lymph nodes are reduced compared to in wild-type mice

• however, the ratio of mature single positive cells to double positive cells is normal

decreased CD8-positive, alpha-beta T cell number ( J:143271 )

• the numbers of mature CD4+ and CD8+ cells in the spleen and lymph nodes are reduced compared to in wild-type mice

• however, the ratio of mature single positive cells to double positive cells is normal

decreased single-positive T cell number ( J:143271 )

• the number of mature single positive T cells is decreased compared to in wild-type mice

increased single-positive T cell number ( J:143271 )

• the number of immature single positive cells in the thymus is increased compared to in wild-type mice

endocrine/exocrine glands

thymus hypoplasia ( J:143271 )

Genotype
MGI:3821709

cn21

• Allelic Composition: Cbfb^tm1Itan/Cbfb^tm1Itan; Zbtb7b^tm2Litt/Zbtb7b^tm1.2Litt; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

hematopoietic system

abnormal T cell differentiation ( J:141144 )

• the number of HSA^hiCD69⁺ and HSA^hi TCRbeta^hi positively selected thymocytes are approximately 20% of those in littermate control or Zbtb7b-deficient mice

decreased CD4-positive, alpha-beta T cell number ( J:141144 )

• absolute numbers of CD4⁺ single positive mature thymocytes are reduced between 5-10 fold compared to controls

• however, the percentage of CD4⁺ T cells in peripheral lymph nodes is only slightly reduced

immune system

abnormal T cell differentiation ( J:141144 )

• the number of HSA^hiCD69⁺ and HSA^hi TCRbeta^hi positively selected thymocytes are approximately 20% of those in littermate control or Zbtb7b-deficient mice

decreased CD4-positive, alpha-beta T cell number ( J:141144 )

• absolute numbers of CD4⁺ single positive mature thymocytes are reduced between 5-10 fold compared to controls

• however, the percentage of CD4⁺ T cells in peripheral lymph nodes is only slightly reduced

Genotype
MGI:3821708

cn22

• Allelic Composition: Cbfb^tm1Itan/Cbfb^tm1Itan; Zbtb7b^tm2Litt/Zbtb7b⁺; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

abnormal T cell differentiation ( J:141144 )

• the number of HSA^hiCD69⁺ and HSA^hi TCRbeta^hi positively selected thymocytes are approximately 20% of those in littermate control or Zbtb7b-deficient mice

decreased CD4-positive, alpha-beta T cell number ( J:141144 )

• absolute numbers of CD4⁺ single positive mature thymocytes are reduced by 5 to 10 fold compared to controls

• however, the percentage of CD4⁺ T cells in peripheral lymph nodes is only slightly reduced

hematopoietic system

abnormal T cell differentiation ( J:141144 )

• the number of HSA^hiCD69⁺ and HSA^hi TCRbeta^hi positively selected thymocytes are approximately 20% of those in littermate control or Zbtb7b-deficient mice

decreased CD4-positive, alpha-beta T cell number ( J:141144 )

• absolute numbers of CD4⁺ single positive mature thymocytes are reduced by 5 to 10 fold compared to controls

• however, the percentage of CD4⁺ T cells in peripheral lymph nodes is only slightly reduced

Genotype
MGI:3821707

cn23

• Allelic Composition: Zbtb7b^tm2Litt/Zbtb7b^tm1.2Litt; Tg(Lck-cre)1Cwi/?
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

hematopoietic system

abnormal T cell differentiation ( J:141144 )

• the number of HSA^hiCD69⁺ and HSA^hi TCRbeta^hi positively selected thymocytes are approximately 20% of those in littermate control or Zbtb7b-deficient mice

decreased CD4-positive, alpha-beta T cell number ( J:141144 )

• absolute numbers of CD4⁺ single positive mature thymocytes are reduced by 5 to 10 fold compared to controls

• the percentage of CD4⁺ T cells in peripheral lymph nodes is reduced by almost 20-fold

immune system

abnormal T cell differentiation ( J:141144 )

• the number of HSA^hiCD69⁺ and HSA^hi TCRbeta^hi positively selected thymocytes are approximately 20% of those in littermate control or Zbtb7b-deficient mice

decreased CD4-positive, alpha-beta T cell number ( J:141144 )

• absolute numbers of CD4⁺ single positive mature thymocytes are reduced by 5 to 10 fold compared to controls

• the percentage of CD4⁺ T cells in peripheral lymph nodes is reduced by almost 20-fold

Genotype
MGI:3701081

cn24

• Allelic Composition: Ikzf1^tm1(Pax5)Mbu/Ikzf1⁺; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

neoplasm

increased T cell derived lymphoma incidence ( J:118111 )

• immature T cell lymphomas develop with shorter latency than in Igh^tm1(PAX5)Mbu homozygotes

endocrine/exocrine glands

increased T cell derived lymphoma incidence ( J:118111 )

• immature T cell lymphomas develop with shorter latency than in Igh^tm1(PAX5)Mbu homozygotes

hematopoietic system

increased T cell derived lymphoma incidence ( J:118111 )

• immature T cell lymphomas develop with shorter latency than in Igh^tm1(PAX5)Mbu homozygotes

immune system

increased T cell derived lymphoma incidence ( J:118111 )

• immature T cell lymphomas develop with shorter latency than in Igh^tm1(PAX5)Mbu homozygotes

Genotype
MGI:3829022

cn25

• Allelic Composition: Ctcf^tm2Gal/Ctcf^tm2Gal; Tg(Lck-cre)1Cwi/0; Tg(TcraH-Y,TcrbH-Y)71Vbo/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * C57BL/10 * DBA/2J

immune system

abnormal T cell differentiation ( J:143271 )

• T cell development is more severely arrested than in Ctcf^tm1Laat/Ctcf^tm1Laat Tg(Lck-cre)1Cwi mice

• mice exhibit a severe block to T cell development at the immature single positive stage

decreased double-positive T cell number ( J:143271 )

• mice exhibit a almost complete lack of double positive cells in the thymus

decreased CD4-positive, alpha-beta T cell number ( J:143271 )

• strongly reduced in the spleen

decreased CD8-positive, alpha-beta T cell number ( J:143271 )

• strongly reduced in the spleen

hematopoietic system

abnormal T cell differentiation ( J:143271 )

• T cell development is more severely arrested than in Ctcf^tm1Laat/Ctcf^tm1Laat Tg(Lck-cre)1Cwi mice

• mice exhibit a severe block to T cell development at the immature single positive stage

decreased double-positive T cell number ( J:143271 )

• mice exhibit a almost complete lack of double positive cells in the thymus

decreased CD4-positive, alpha-beta T cell number ( J:143271 )

• strongly reduced in the spleen

decreased CD8-positive, alpha-beta T cell number ( J:143271 )

• strongly reduced in the spleen

Genotype
MGI:5297085

cn26

• Allelic Composition: Tnfsf11^tm1.1Htaka/Tnfsf11^tm1.2Htaka; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * C57BL/6J

skeleton

skeleton phenotype ( J:177557 )

N • mice do not exhibit an osteopetrotic phenotype unlike null mice

Genotype
MGI:3047333

cn27

• Allelic Composition: Myb^tm1Cgn/Myb^tm1Cgn; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * DBA/2

hematopoietic system

thymus hypoplasia ( J:91122 )

• thymus cellularity is reduced about 97%

decreased double-negative T cell number ( J:91122 )

• the absolute number of double negative cells is decreased about 40% however the proportion of double negative cells is increased to about 60% of total thymocytes

• about 90% of the double negative cells are CD44^-CD25⁺ DN3 cells

decreased double-positive T cell number ( J:91122 )

• the absolute number of double positive cells is greatly decreased

arrested T cell differentiation ( J:91122 )

• a block in thymocyte maturation at the DN3 stage or in the transition from DN3 to DN4 appears to occur

decreased CD4-positive, alpha-beta T cell number ( J:91122 )

• the absolute number of CD4 single positive cells is greatly decreased

decreased CD8-positive, alpha-beta T cell number ( J:91122 )

• the absolute number of CD8 single positive cells is greatly decreased

decreased gamma-delta T cell number ( J:91122 )

• the number of gamma delta TCR bearing thymocytes is decreased

immune system

thymus hypoplasia ( J:91122 )

• thymus cellularity is reduced about 97%

decreased double-negative T cell number ( J:91122 )

• the absolute number of double negative cells is decreased about 40% however the proportion of double negative cells is increased to about 60% of total thymocytes

• about 90% of the double negative cells are CD44^-CD25⁺ DN3 cells

decreased double-positive T cell number ( J:91122 )

• the absolute number of double positive cells is greatly decreased

arrested T cell differentiation ( J:91122 )

• a block in thymocyte maturation at the DN3 stage or in the transition from DN3 to DN4 appears to occur

decreased CD4-positive, alpha-beta T cell number ( J:91122 )

• the absolute number of CD4 single positive cells is greatly decreased

decreased CD8-positive, alpha-beta T cell number ( J:91122 )

• the absolute number of CD8 single positive cells is greatly decreased

decreased gamma-delta T cell number ( J:91122 )

• the number of gamma delta TCR bearing thymocytes is decreased

endocrine/exocrine glands

thymus hypoplasia ( J:91122 )

• thymus cellularity is reduced about 97%

Genotype
MGI:4839039

cn28

• Allelic Composition: Nkap^tm1.1Viss/Y; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

immune system

thymus hypoplasia ( J:165961 )

♂

decreased thymocyte number ( J:165961 )

♂ • 10-fold

decreased DN4 thymocyte number ( J:165961 )

♂

increased DN3 thymocyte number ( J:165961 )

♂ • mice exhibit a disproportionate increased (2-fold) in DN3 thymocytes compared to in wild-type mice

decreased double-positive T cell number ( J:165961 )

♂

arrested T cell differentiation ( J:165961 )

♂ • alpha beta T cell development is incompletely blocked at the DN3 stage unlike in wild-type mice

♂ • however, delta gamma T cell development and pre-TCR signaling are normal

decreased CD4-positive, alpha-beta T cell number ( J:165961 )

♂

decreased CD8-positive, alpha-beta T cell number ( J:165961 )

♂

hematopoietic system

thymus hypoplasia ( J:165961 )

♂

decreased thymocyte number ( J:165961 )

♂ • 10-fold

decreased DN4 thymocyte number ( J:165961 )

♂

increased DN3 thymocyte number ( J:165961 )

♂ • mice exhibit a disproportionate increased (2-fold) in DN3 thymocytes compared to in wild-type mice

decreased double-positive T cell number ( J:165961 )

♂

arrested T cell differentiation ( J:165961 )

♂ • alpha beta T cell development is incompletely blocked at the DN3 stage unlike in wild-type mice

♂ • however, delta gamma T cell development and pre-TCR signaling are normal

decreased CD4-positive, alpha-beta T cell number ( J:165961 )

♂

decreased CD8-positive, alpha-beta T cell number ( J:165961 )

♂

endocrine/exocrine glands

thymus hypoplasia ( J:165961 )

♂

decreased thymocyte number ( J:165961 )

♂ • 10-fold

decreased DN4 thymocyte number ( J:165961 )

♂

increased DN3 thymocyte number ( J:165961 )

♂ • mice exhibit a disproportionate increased (2-fold) in DN3 thymocytes compared to in wild-type mice

Genotype
MGI:5691371

cn29

• Allelic Composition: Vdac2^tm1.1Ehyc/Vdac2^tm1.1Ehyc; Bak1^tm1Thsn/Bak1^tm1Thsn; Tg(Lck-cre)1Cwi/?
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2

immune system

immune system phenotype ( J:220951 )

N • thymus size normal

N • thymocyte number normal

N • no increase in apoptosis

Genotype
MGI:5691376

cn30

• Allelic Composition: Bak1^tm1Thsn/Bak1^tm1Thsn; Bax^tm2Sjk/Bax^tm2Sjk; Tg(Lck-cre)1Cwi/?
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2

immune system

decreased thymocyte apoptosis ( J:220951 )

• completely resistant to apoptosis

cellular

decreased thymocyte apoptosis ( J:220951 )

• completely resistant to apoptosis

hematopoietic system

decreased thymocyte apoptosis ( J:220951 )

• completely resistant to apoptosis

endocrine/exocrine glands

decreased thymocyte apoptosis ( J:220951 )

• completely resistant to apoptosis

Genotype
MGI:3056654

cn31

• Allelic Composition: Igbp1^tm1Cbt/Igbp1⁺; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2

hematopoietic system

decreased thymocyte number ( J:93221 )

♀ • thymocyte numbers are reduced about 60% however peripheral T cell numbers are normal

immune system

decreased thymocyte number ( J:93221 )

♀ • thymocyte numbers are reduced about 60% however peripheral T cell numbers are normal

endocrine/exocrine glands

decreased thymocyte number ( J:93221 )

♀ • thymocyte numbers are reduced about 60% however peripheral T cell numbers are normal

Genotype
MGI:3056653

cn32

• Allelic Composition: Igbp1^tm1Cbt/Y; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2

hematopoietic system

decreased thymocyte number ( J:93221 )

♂ • the thymus is depleted of developing T cells and the residual cells are relatively enriched with immature thymocytes

♂ • no peripheral T cells are seen

immune system

decreased thymocyte number ( J:93221 )

♂ • the thymus is depleted of developing T cells and the residual cells are relatively enriched with immature thymocytes

♂ • no peripheral T cells are seen

endocrine/exocrine glands

decreased thymocyte number ( J:93221 )

♂ • the thymus is depleted of developing T cells and the residual cells are relatively enriched with immature thymocytes

♂ • no peripheral T cells are seen

Genotype
MGI:3713384

cn33

• Allelic Composition: Chd4^tm1.1Kge/Chd4^tm1.2Kge; Tg(CAG-Bgeo/GFP)21Lbe/0; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL

cellular

abnormal cell cycle ( J:109547 )

• progressive decrease in cycling T cells is observed from second to third cell division in vitro, along with large number of nonblast T cells that appear to fail to enter cell cycle

decreased T cell proliferation ( J:109547 )

• a 5- to 7-fold decrease in proliferation is observed relative to wild-type cells in vitro after 48 hours of TCR/CD3 stimulation; fewer mutant cells are in S phase

immune system

decreased T cell proliferation ( J:109547 )

• a 5- to 7-fold decrease in proliferation is observed relative to wild-type cells in vitro after 48 hours of TCR/CD3 stimulation; fewer mutant cells are in S phase

hematopoietic system

decreased T cell proliferation ( J:109547 )

• a 5- to 7-fold decrease in proliferation is observed relative to wild-type cells in vitro after 48 hours of TCR/CD3 stimulation; fewer mutant cells are in S phase

Genotype
MGI:3839884

cn34

• Allelic Composition: Kmt2a^tm1.1Erns/Kmt2a^tm1.1Erns; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129S2/SvPas * C57BL/6 * SJL

immune system

immune system phenotype ( J:147265 )

N • mice exhibit normally differentiating T, B, and myeloid cells

Genotype
MGI:4367754

cn35

• Allelic Composition: Foxo1^tm1.1Stlp/Foxo1^tm1.1Stlp; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129S4/SvJae

immune system

decreased thymocyte apoptosis ( J:154205 )

• half as many CD4+ T cells undergo apoptosis compared to in wild-type mice

decreased T cell proliferation ( J:154205 )

• proliferation of single positive thymocytes stimulated with anti-CD3 and anti-CD3 plus anti-CD28 is almost half as much as in similarly treated wild-type cells

increased CD4-positive, alpha-beta T cell number ( J:154205 )

• CD4+ T cell thymocytes are increased compared to in wild-type mice

increased CD8-positive, alpha-beta T cell number ( J:154205 )

• CD8+ T cell thymocytes are increased compared to in wild-type mice

decreased T cell number ( J:154205 )

• spleen and lymph nodes have half as many T cells as in wild-type mice

decreased double-positive T cell number ( J:154205 )

• the percent of double positive thymocytes is slightly lower percent than in wild-type mice

decreased CD8-positive, alpha-beta T cell number ( J:154205 )

• the percentage of CD8+ T cells is decreased compared to in wild-type mice

abnormal single-positive T cell number ( J:154205 )

• the ratio of CD4+ to CD8+ peripheral T cells is skewed in favor of CD4+ T cells unlike in wild-type mice

small spleen ( J:154205 )

• with less than half of T cells

small lymphoid organs ( J:154205 )

• with less than half of T cells

hematopoietic system

decreased thymocyte apoptosis ( J:154205 )

• half as many CD4+ T cells undergo apoptosis compared to in wild-type mice

decreased T cell proliferation ( J:154205 )

• proliferation of single positive thymocytes stimulated with anti-CD3 and anti-CD3 plus anti-CD28 is almost half as much as in similarly treated wild-type cells

increased CD4-positive, alpha-beta T cell number ( J:154205 )

• CD4+ T cell thymocytes are increased compared to in wild-type mice

increased CD8-positive, alpha-beta T cell number ( J:154205 )

• CD8+ T cell thymocytes are increased compared to in wild-type mice

decreased T cell number ( J:154205 )

• spleen and lymph nodes have half as many T cells as in wild-type mice

decreased double-positive T cell number ( J:154205 )

• the percent of double positive thymocytes is slightly lower percent than in wild-type mice

decreased CD8-positive, alpha-beta T cell number ( J:154205 )

• the percentage of CD8+ T cells is decreased compared to in wild-type mice

abnormal single-positive T cell number ( J:154205 )

• the ratio of CD4+ to CD8+ peripheral T cells is skewed in favor of CD4+ T cells unlike in wild-type mice

small spleen ( J:154205 )

• with less than half of T cells

cellular

decreased thymocyte apoptosis ( J:154205 )

• half as many CD4+ T cells undergo apoptosis compared to in wild-type mice

decreased T cell proliferation ( J:154205 )

• proliferation of single positive thymocytes stimulated with anti-CD3 and anti-CD3 plus anti-CD28 is almost half as much as in similarly treated wild-type cells

endocrine/exocrine glands

decreased thymocyte apoptosis ( J:154205 )

• half as many CD4+ T cells undergo apoptosis compared to in wild-type mice

Genotype
MGI:4943254

cn36

• Allelic Composition: Fadd^tm1Wnt/Fadd^tm1Wnt; Tg(Fadd/EGFP)#Jizh/?; Tg(Lck-cre)1Cwi/?
• Genetic Background: involves: 129S4/SvJae

cellular

decreased sensitivity to induced cell death ( J:113207 )

• anti-Fas antibody fails to induce cell death in T-cells as it does in controls

• resistant to FasL induced cell death

immune system

immune system phenotype ( J:113207 )

N • thymus, spleen, and lymph nodes all appear to be normal

increased B cell number ( J:113207 )

• peripheral B-cells are increased in number

decreased activated T cell number ( J:113207 )

• TCR activation of T-cells is reduced

decreased single-positive T cell number ( J:113207 )

• both CD4+ and CD8+ peripheral T-cells are reduced in number

• peripheral T-cells divide less efficiently when transplanted to Rag1 deficient mice

hematopoietic system

increased B cell number ( J:113207 )

• peripheral B-cells are increased in number

decreased activated T cell number ( J:113207 )

• TCR activation of T-cells is reduced

decreased single-positive T cell number ( J:113207 )

• both CD4+ and CD8+ peripheral T-cells are reduced in number

• peripheral T-cells divide less efficiently when transplanted to Rag1 deficient mice

Genotype
MGI:4430744

cn37

• Allelic Composition: Trp53^tm6Xu/Trp53^tm6Xu; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129S4/SvJae

mortality/aging

decreased tumor-free survival time ( J:157041 )

• all die of tumors by 50 weeks of age

neoplasm

increased metastatic potential ( J:157041 )

• unlike in thymocyte conditional null mice (carrying Trp53^tm1Brd and Tg(Lck-cre)1Cwi ), about 40% of tumors are metastatic

increased lymphoma incidence ( J:157041 )

• predominantly peripheral and thymic lymphomas by 50 weeks of age

increased T cell derived lymphoma incidence ( J:157041 )

• by 50 weeks of age

endocrine/exocrine glands

increased T cell derived lymphoma incidence ( J:157041 )

• by 50 weeks of age

hematopoietic system

increased T cell derived lymphoma incidence ( J:157041 )

• by 50 weeks of age

immune system

increased T cell derived lymphoma incidence ( J:157041 )

• by 50 weeks of age

Genotype
MGI:4839179

cn38

• Allelic Composition: Tox^tm1Kay/Tox^tm1Kay; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

immune system

abnormal CD4-positive T cell differentiation ( J:164687 )

• the T cell defect seen in germline null mice is recapitulated in these mice

• however, all lymph nodes are present

hematopoietic system

abnormal CD4-positive T cell differentiation ( J:164687 )

• the T cell defect seen in germline null mice is recapitulated in these mice

• however, all lymph nodes are present

Genotype
MGI:3760284

cn39

• Allelic Composition: Was^tm1Sbs/Was^tm1Sbs; Wasl^tm2Sbs/Wasl^tm2Sbs; Tg(Lck-cre)1Cwi/?
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

immune system

colitis ( J:125309 )

♀ • most mice develop signs of colitis by 2 months of age with elongation of crypts and mucosal thickening

abnormal thymocyte activation ( J:125309 )

♀ • unlike wild-type thymocytes, single positive (SP) thymocytes fail to proliferate after CD3 stimulation

♀ • spreading of TCR^high CD4 or CD8 SP thymocytes on a surface covered with antibodies to CD3 and CD28 is reduced

♀ • migratory responses to CCL19 or CXCL12 are reduced even more than in Was^tm1Sbs homozygotes and compared to in wild-type cells

abnormal double-negative T cell morphology ( J:125309 )

♀ • mice exhibit a reduction in large and cycling double negative 3 (DN3) cells and, to a lesser degree, DN4 cells

♀ • the ratio of DN3 to DN4 is skewed (3.18+/-1.89 compared to 0.74+/-0.45 in wild-type mice and 1.49+/-0.90 in Was^tm1Sbs homozygotes)

abnormal double-positive T cell morphology ( J:125309 )

♀ • the percent of double positive (DP) CD69^hi cells is greater than in wild-type

decreased T cell number ( J:125309 )

♀ • the numbers of CD4+ and CD8+ T lymphocytes is reduced

decreased thymocyte number ( J:125309 )

♀ • the number of thymocytes is reduced

♀ • however, there is no increase in apoptosis

decreased regulatory T cell number ( J:125309 )

♀

decreased single-positive T cell number ( J:125309 )

♀ • mice have fewer single positive CD69^hi cells compared to in wild-type mice and Was^tm1Sbs homozygotes

increased single-positive T cell number ( J:125309 )

♀ • the number of single positive CD69^low CD62L^low cells is increased compared to in wild-type mice

hematopoietic system

abnormal thymocyte activation ( J:125309 )

♀ • unlike wild-type thymocytes, single positive (SP) thymocytes fail to proliferate after CD3 stimulation

♀ • spreading of TCR^high CD4 or CD8 SP thymocytes on a surface covered with antibodies to CD3 and CD28 is reduced

♀ • migratory responses to CCL19 or CXCL12 are reduced even more than in Was^tm1Sbs homozygotes and compared to in wild-type cells

abnormal double-negative T cell morphology ( J:125309 )

♀ • mice exhibit a reduction in large and cycling double negative 3 (DN3) cells and, to a lesser degree, DN4 cells

♀ • the ratio of DN3 to DN4 is skewed (3.18+/-1.89 compared to 0.74+/-0.45 in wild-type mice and 1.49+/-0.90 in Was^tm1Sbs homozygotes)

abnormal double-positive T cell morphology ( J:125309 )

♀ • the percent of double positive (DP) CD69^hi cells is greater than in wild-type

decreased T cell number ( J:125309 )

♀ • the numbers of CD4+ and CD8+ T lymphocytes is reduced

decreased thymocyte number ( J:125309 )

♀ • the number of thymocytes is reduced

♀ • however, there is no increase in apoptosis

decreased regulatory T cell number ( J:125309 )

♀

decreased single-positive T cell number ( J:125309 )

♀ • mice have fewer single positive CD69^hi cells compared to in wild-type mice and Was^tm1Sbs homozygotes

increased single-positive T cell number ( J:125309 )

♀ • the number of single positive CD69^low CD62L^low cells is increased compared to in wild-type mice

digestive/alimentary system

abnormal crypts of Lieberkuhn morphology ( J:125309 )

♀ • most mice develop signs of colitis by 2 months of age with elongation of crypts and mucosal thickening

colitis ( J:125309 )

♀ • most mice develop signs of colitis by 2 months of age with elongation of crypts and mucosal thickening

endocrine/exocrine glands

abnormal crypts of Lieberkuhn morphology ( J:125309 )

♀ • most mice develop signs of colitis by 2 months of age with elongation of crypts and mucosal thickening

decreased thymocyte number ( J:125309 )

♀ • the number of thymocytes is reduced

♀ • however, there is no increase in apoptosis

abnormal thymocyte activation ( J:125309 )

♀ • unlike wild-type thymocytes, single positive (SP) thymocytes fail to proliferate after CD3 stimulation

♀ • spreading of TCR^high CD4 or CD8 SP thymocytes on a surface covered with antibodies to CD3 and CD28 is reduced

♀ • migratory responses to CCL19 or CXCL12 are reduced even more than in Was^tm1Sbs homozygotes and compared to in wild-type cells

Genotype
MGI:5485995

cn40

• Allelic Composition: Ptpmt1^tm2.1Ckq/Ptpmt1^tm2.1Ckq; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * C57BL/6J * SJL

hematopoietic system

hematopoietic system phenotype ( J:194929 )

N • mice exhibit normal myeloid, T lymphoid and B lymphoid lineages and cell cycle of lineage progenitors

immune system

immune system phenotype ( J:194929 )

N • mice exhibit normal myeloid, T lymphoid and B lymphoid lineages and cell cycle of lineage progenitors

Genotype
MGI:3044045

cn41

• Allelic Composition: Ppp3r1^tm2Grc/Ppp3r1^tm2Grc; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * DBA/2

hematopoietic system

thymus hypoplasia ( J:89771 )

• general decrease in cellularity is observed

decreased thymocyte number ( J:89771 )

• specific reduction in CD4+CD8- and CD4-CD8+ single positive (SP) thymocytes is found

immune system

thymus hypoplasia ( J:89771 )

• general decrease in cellularity is observed

decreased thymocyte number ( J:89771 )

• specific reduction in CD4+CD8- and CD4-CD8+ single positive (SP) thymocytes is found

endocrine/exocrine glands

thymus hypoplasia ( J:89771 )

• general decrease in cellularity is observed

decreased thymocyte number ( J:89771 )

• specific reduction in CD4+CD8- and CD4-CD8+ single positive (SP) thymocytes is found

Genotype
MGI:3590232

cn42

• Allelic Composition: Apc^tm1Kk/Apc^tm1Kk; Tg(Lck-cre)1Cwi/?
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * DBA/2

immune system

small thymus ( J:100461 )

thymus hypoplasia ( J:100461 )

• 1/8 to 1/10 as many thymocytes as in controls

abnormal T cell differentiation ( J:100461 )

• higher levels of apoptosis in DN3, DN4, and DP cells

increased double-negative T cell number ( J:100461 )

• 50% of thymocytes with a double negative phenotype

arrested T cell differentiation ( J:100461 )

• T cell development is blocked at the DN4 stage

increased mast cell number ( J:128606 )

• total mononuclear cell preparations from the intestine shows at least a 9-fold higher frequency of mast cell progenitors than wild-type mice

• treatment of mice with anti-TNFalpha antibody lowers the numbers of mast cell progenitors in the intestine

increased circulating tumor necrosis factor level ( J:128606 )

• 10-fold increase in serum TNFalpha levels by 4 months of age but declines to 3-fold increase in late-stage polyposis

hematopoietic system

small thymus ( J:100461 )

thymus hypoplasia ( J:100461 )

• 1/8 to 1/10 as many thymocytes as in controls

abnormal T cell differentiation ( J:100461 )

• higher levels of apoptosis in DN3, DN4, and DP cells

increased double-negative T cell number ( J:100461 )

• 50% of thymocytes with a double negative phenotype

arrested T cell differentiation ( J:100461 )

• T cell development is blocked at the DN4 stage

increased mast cell number ( J:128606 )

• total mononuclear cell preparations from the intestine shows at least a 9-fold higher frequency of mast cell progenitors than wild-type mice

• treatment of mice with anti-TNFalpha antibody lowers the numbers of mast cell progenitors in the intestine

endocrine/exocrine glands

small thymus ( J:100461 )

thymus hypoplasia ( J:100461 )

• 1/8 to 1/10 as many thymocytes as in controls

digestive/alimentary system

abnormal intestinal mucosa morphology ( J:104334 )

• dysplastic crypts with hyperchromatic nuclei, expanding at the luminal surface of the mucosa, neighboring well spaced, nondysplastic crypts and villi

increased intestinal adenoma incidence ( J:104334 )

• mice show adenomas in the small and large intestine from 2 months of age which typically have a tubovillous appearance

• adenomas are devoid of differentiated cells

intestine polyps ( J:128606 )

• intestinal lesions are seen as early as 2 months after birth and mice develop adenomatous polyps that are typically infiltrated with intraepithelial mast cells restricted to the lesions

• mice treated with anti-TNFalpha antibody every 2 days for 2 weeks show fewer polyps than controls and the polyps that persist have a more flattened and differentiated morphology and are smaller, and the mean vessel volume in the polyps is 4-times less than in untreated mice

homeostasis/metabolism

increased circulating tumor necrosis factor level ( J:128606 )

• 10-fold increase in serum TNFalpha levels by 4 months of age but declines to 3-fold increase in late-stage polyposis

neoplasm

increased intestinal adenoma incidence ( J:104334 )

• mice show adenomas in the small and large intestine from 2 months of age which typically have a tubovillous appearance

• adenomas are devoid of differentiated cells

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
familial adenomatous polyposis		DOID:0050424	OMIM:PS175100	J:128606

Genotype
MGI:3044044

cn43

• Allelic Composition: Ppp3r1^tm2Grc/Ppp3r1^tm2.1Grc; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * DBA/2

hematopoietic system

abnormal positive T cell selection ( J:89771 )

• double positive (DP) thymocytes cannot progress to the single positive (SP)

• DP thymocytes show defects in induction of TCRb, CD69, and CD5 which are markers of positive selection

• negative selection was unaffected

abnormal splenic cell ratio ( J:89771 )

• decreased numbers of T lymphocytes compared to controls

immune system

abnormal positive T cell selection ( J:89771 )

• double positive (DP) thymocytes cannot progress to the single positive (SP)

• DP thymocytes show defects in induction of TCRb, CD69, and CD5 which are markers of positive selection

• negative selection was unaffected

abnormal splenic cell ratio ( J:89771 )

• decreased numbers of T lymphocytes compared to controls

abnormal lymph node cell ratio ( J:89771 )

• decreased numbers of T lymphocytes compared to controls

homeostasis/metabolism

abnormal enzyme/coenzyme activity ( J:89771 )

• ERK activation is defective in Ppp3r1-deficient animals

Genotype
MGI:5295469

cn44

• Allelic Composition: Ptpn11^tm1Ckq/Ptpn11⁺; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6J

endocrine/exocrine glands

increased lymphoblastic lymphoma incidence ( J:177285 )

• T cell lymphoblastic leukemia/lymphoma in 8 of 15 mice

mortality/aging

premature death ( J:177285 )

neoplasm

increased lymphoblastic lymphoma incidence ( J:177285 )

• T cell lymphoblastic leukemia/lymphoma in 8 of 15 mice

increased acute lymphoblastic leukemia incidence ( J:177285 )

• T cell lymphoblastic leukemia/lymphoma in 8 of 15 mice

immune system

immune system phenotype ( J:177285 )

N • T and B cell development is normal

increased lymphoblastic lymphoma incidence ( J:177285 )

• T cell lymphoblastic leukemia/lymphoma in 8 of 15 mice

hematopoietic system

increased lymphoblastic lymphoma incidence ( J:177285 )

• T cell lymphoblastic leukemia/lymphoma in 8 of 15 mice

Genotype
MGI:5485404

cn45

• Allelic Composition: Hdac1^tm1.1Shmc/Hdac1^tm1.1Shmc; Hdac2^tm1.1Shmc/Hdac2⁺; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129S7/SvEvBrd

mortality/aging

premature death ( J:194769 )

• all mice die at an average of 15 weeks

immune system

enlarged thymus ( J:194769 )

• in diseased mice

thymus hypoplasia ( J:194769 )

• 5-fold reduction in cellularity in neonates

enlarged spleen ( J:194769 )

• in diseased mice

increased double-negative T cell number ( J:194769 )

• in the percentage but not the absolute numbers

decreased double-positive T cell number ( J:194769 )

increased double-positive T cell number ( J:194769 )

• increase in CD4^low/CD8^high cells at 6 to 8 weeks

arrested T cell differentiation ( J:194769 )

• block at the double negative to double positive transition

decreased CD4-positive, alpha-beta T cell number ( J:194769 )

decreased CD8-positive, alpha-beta T cell number ( J:194769 )

increased single-positive T cell number ( J:194769 )

• 4-fold increase in the percent of immature single positive cells at 6 to 8 weeks

neoplasm

increased tumor incidence ( J:194769 )

• in 1 of 8 mice

growth/size/body

enlarged chest ( J:194769 )

• in moribund mice

distended abdomen ( J:194769 )

• in moribund mice

enlarged spleen ( J:194769 )

• in diseased mice

respiratory system

increased pulmonary respiratory rate ( J:194769 )

• in moribund mice

hematopoietic system

enlarged thymus ( J:194769 )

• in diseased mice

thymus hypoplasia ( J:194769 )

• 5-fold reduction in cellularity in neonates

enlarged spleen ( J:194769 )

• in diseased mice

increased double-negative T cell number ( J:194769 )

• in the percentage but not the absolute numbers

decreased double-positive T cell number ( J:194769 )

increased double-positive T cell number ( J:194769 )

• increase in CD4^low/CD8^high cells at 6 to 8 weeks

arrested T cell differentiation ( J:194769 )

• block at the double negative to double positive transition

decreased CD4-positive, alpha-beta T cell number ( J:194769 )

decreased CD8-positive, alpha-beta T cell number ( J:194769 )

increased single-positive T cell number ( J:194769 )

• 4-fold increase in the percent of immature single positive cells at 6 to 8 weeks

endocrine/exocrine glands

enlarged thymus ( J:194769 )

• in diseased mice

thymus hypoplasia ( J:194769 )

• 5-fold reduction in cellularity in neonates

Genotype
MGI:5485403

cn46

• Allelic Composition: Hdac1^tm1.1Shmc/Hdac1^tm1.1Shmc; Hdac2^tm1.1Shmc/Hdac2^tm1.1Shmc; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129S7/SvEvBrd

mortality/aging

premature death ( J:194769 )

• all mice die at an average of 15 weeks

immune system

increased thymocyte apoptosis ( J:194769 )

• in neonates and at 6 weeks

increased T cell proliferation ( J:194769 )

• in the thymus and spleen

abnormal thymus corticomedullary boundary morphology ( J:194769 )

• effacement of medullary and cortical substructures in diseased thymus

enlarged thymus ( J:194769 )

• massively in diseased mice due to overpopulation with immature and double positive T cells

decreased thymocyte number ( J:194769 )

• 2-fold reduction in mature TCRbeta^high/CD5^high thymocytes at 10 to 14 days

increased thymocyte number ( J:194769 )

• 3-fold increase immature TCRbeta^int/CD5^low thymocytes at 10 to 14 days

increased T cell derived lymphoma incidence ( J:194769 )

enlarged spleen ( J:194769 )

• massively in diseased mice due to overpopulation with immature and double positive T cells

decreased double-positive T cell number ( J:194769 )

• 2.6-fold reduction at 10 to 14 days

increased double-positive T cell number ( J:194769 )

• increase in CD4^low/CD8^high cells at 6 to 8 weeks

• 10-fold increase in absolute number of CD4^low/CD8^high cells at 10 to 14 days

arrested T cell differentiation ( J:194769 )

• block at the double negative to double positive transition

• defects in positive selection and CD4 lineage commitment

increased single-positive T cell number ( J:194769 )

• 4-fold increase in the percent of immature single positive cells at 6 to 8 weeks

neoplasm

increased tumor incidence ( J:194769 )

• with general hyperproliferation of immature T cells and infiltration into nonlymphatic organs (lung, liver and kidney)

increased T cell derived lymphoma incidence ( J:194769 )

growth/size/body

enlarged chest ( J:194769 )

• in moribund mice

distended abdomen ( J:194769 )

• in moribund mice

enlarged spleen ( J:194769 )

• massively in diseased mice due to overpopulation with immature and double positive T cells

respiratory system

increased pulmonary respiratory rate ( J:194769 )

• in moribund mice

cellular

aneuploidy ( J:194769 )

• in tumor cells

trisomy ( J:194769 )

• in tumor cells

increased thymocyte apoptosis ( J:194769 )

• in neonates and at 6 weeks

increased T cell proliferation ( J:194769 )

• in the thymus and spleen

chromosomal instability ( J:194769 )

• in tumorigenic T cells

hematopoietic system

increased thymocyte apoptosis ( J:194769 )

• in neonates and at 6 weeks

increased T cell proliferation ( J:194769 )

• in the thymus and spleen

abnormal thymus corticomedullary boundary morphology ( J:194769 )

• effacement of medullary and cortical substructures in diseased thymus

enlarged thymus ( J:194769 )

• massively in diseased mice due to overpopulation with immature and double positive T cells

decreased thymocyte number ( J:194769 )

• 2-fold reduction in mature TCRbeta^high/CD5^high thymocytes at 10 to 14 days

increased thymocyte number ( J:194769 )

• 3-fold increase immature TCRbeta^int/CD5^low thymocytes at 10 to 14 days

increased T cell derived lymphoma incidence ( J:194769 )

enlarged spleen ( J:194769 )

• massively in diseased mice due to overpopulation with immature and double positive T cells

decreased double-positive T cell number ( J:194769 )

• 2.6-fold reduction at 10 to 14 days

increased double-positive T cell number ( J:194769 )

• increase in CD4^low/CD8^high cells at 6 to 8 weeks

• 10-fold increase in absolute number of CD4^low/CD8^high cells at 10 to 14 days

arrested T cell differentiation ( J:194769 )

• block at the double negative to double positive transition

• defects in positive selection and CD4 lineage commitment

increased single-positive T cell number ( J:194769 )

• 4-fold increase in the percent of immature single positive cells at 6 to 8 weeks

endocrine/exocrine glands

increased thymocyte apoptosis ( J:194769 )

• in neonates and at 6 weeks

abnormal thymus corticomedullary boundary morphology ( J:194769 )

• effacement of medullary and cortical substructures in diseased thymus

enlarged thymus ( J:194769 )

• massively in diseased mice due to overpopulation with immature and double positive T cells

decreased thymocyte number ( J:194769 )

• 2-fold reduction in mature TCRbeta^high/CD5^high thymocytes at 10 to 14 days

increased thymocyte number ( J:194769 )

• 3-fold increase immature TCRbeta^int/CD5^low thymocytes at 10 to 14 days

increased T cell derived lymphoma incidence ( J:194769 )

Genotype
MGI:5485402

cn47

• Allelic Composition: Hdac1^tm1.1Shmc/Hdac1⁺; Hdac2^tm1.1Shmc/Hdac2^tm1.1Shmc; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129S7/SvEvBrd

immune system

immune system phenotype ( J:194769 )

N • mice exhibit normal numbers of double negative, double positive, CD4 single positive and CD8 single positive T cells

Genotype
MGI:5485401

cn48

• Allelic Composition: Hdac2^tm1.1Shmc/Hdac2^tm1.1Shmc; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129S7/SvEvBrd

immune system

immune system phenotype ( J:194769 )

N • mice exhibit normal numbers of double negative, double positive, CD4 single positive and CD8 single positive T cells

Genotype
MGI:5485400

cn49

• Allelic Composition: Hdac1^tm1.1Shmc/Hdac1^tm1.1Shmc; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129S7/SvEvBrd

immune system

immune system phenotype ( J:194769 )

N • mice exhibit normal numbers of double negative, double positive, CD4 single positive and CD8 single positive T cells

Genotype
MGI:4430745

cn50

• Allelic Composition: Trp53^tm1Brd/Trp53^tm1Brd; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129S7/SvEvBrd

mortality/aging

premature death ( J:157041 )

• all die of thymic lymphomas by 50 weeks of age

neoplasm

increased T cell derived lymphoma incidence ( J:157041 )

• in all mice by 50 weeks of age

endocrine/exocrine glands

increased T cell derived lymphoma incidence ( J:157041 )

• in all mice by 50 weeks of age

hematopoietic system

increased T cell derived lymphoma incidence ( J:157041 )

• in all mice by 50 weeks of age

immune system

increased T cell derived lymphoma incidence ( J:157041 )

• in all mice by 50 weeks of age

Genotype
MGI:2677147

cn51

• Allelic Composition: Smarca4^tm1.2Pcn/Smarca4^tm1Mag; Tg(Lck-cre)1Cwi/?
• Genetic Background: involves: 129S/Sv * C57BL/6 * DBA/2

hematopoietic system

thymus hypoplasia ( J:85191 )

• 40-100 fold reduction in cell numbers in the thymus

abnormal T cell differentiation ( J:85191 )

decreased double-negative T cell number ( J:85191 )

decreased double-positive T cell number ( J:85191 )

• absence of double positive T cells

arrested T cell differentiation ( J:85191 )

• T cells become arrested when cre inactivation occurs primarily at DN4 stage but sometimes earlier

• leads to expression of CD4 and to death of T cells

decreased CD4-positive, alpha-beta T cell number ( J:85191 )

• very small numbers of CD4+ cells

absent CD8-positive, alpha-beta T cells ( J:85191 )

• absence of CD8+ cells

immune system

thymus hypoplasia ( J:85191 )

• 40-100 fold reduction in cell numbers in the thymus

abnormal T cell differentiation ( J:85191 )

decreased double-negative T cell number ( J:85191 )

decreased double-positive T cell number ( J:85191 )

• absence of double positive T cells

arrested T cell differentiation ( J:85191 )

• T cells become arrested when cre inactivation occurs primarily at DN4 stage but sometimes earlier

• leads to expression of CD4 and to death of T cells

decreased CD4-positive, alpha-beta T cell number ( J:85191 )

• very small numbers of CD4+ cells

absent CD8-positive, alpha-beta T cells ( J:85191 )

• absence of CD8+ cells

endocrine/exocrine glands

thymus hypoplasia ( J:85191 )

• 40-100 fold reduction in cell numbers in the thymus

Genotype
MGI:2677148

cn52

• Allelic Composition: Smarca4^tm1.2Pcn/Smarca4^tm1Mag; Tg(Lck-cre)1Cwi/?; Tg(LCKprBCL2)36Sjk/?
• Genetic Background: involves: 129S/Sv * C57BL/6 * DBA/2

hematopoietic system

arrested T cell differentiation ( J:85191 )

• cells arrested in G1 phase of cell cycle leading to a 10 fold increase in cell numbers as opposed to animals lacking TgN(LCKprBCL2)36Sjk

immune system

arrested T cell differentiation ( J:85191 )

• cells arrested in G1 phase of cell cycle leading to a 10 fold increase in cell numbers as opposed to animals lacking TgN(LCKprBCL2)36Sjk

Genotype
MGI:5141759

cn53

• Allelic Composition: Rag2^tm1Fwa/Rag2^tm1Fwa; Sos1^tm1.1Les/Sos1^tm1.1Les; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129S/SvEv * C57BL/6

immune system

decreased T cell proliferation ( J:174536 )

• marked reduction in both proliferation and differentiation into DP cells on a per cell basis

abnormal T cell differentiation ( J:174536 )

• marked reduction in both proliferation and differentiation into DP cells on a per cell basis

hematopoietic system

decreased T cell proliferation ( J:174536 )

• marked reduction in both proliferation and differentiation into DP cells on a per cell basis

abnormal T cell differentiation ( J:174536 )

• marked reduction in both proliferation and differentiation into DP cells on a per cell basis

cellular

decreased T cell proliferation ( J:174536 )

• marked reduction in both proliferation and differentiation into DP cells on a per cell basis

Genotype
MGI:3028491

cn54

• Allelic Composition: Birc5^tm1Wnt/Birc5^tm1Emc; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129S/SvEv * C57BL/6 * DBA/2

hematopoietic system

thymus hypoplasia ( J:87435 )

• thymic cellularity reduced to 1/8 normal

increased double-negative T cell number ( J:87435 )

• most thymocytes are double negative

• a lower percentage of double negative stage 4 cells (DN4) and increase in DN3 cells

decreased T cell number ( J:87435 )

• only moderate reductions in mature T cells in spleen and lymph nodes

decreased double-positive T cell number ( J:87435 )

• absolute numbers of double positive cells reduced

immune system

thymus hypoplasia ( J:87435 )

• thymic cellularity reduced to 1/8 normal

increased double-negative T cell number ( J:87435 )

• most thymocytes are double negative

• a lower percentage of double negative stage 4 cells (DN4) and increase in DN3 cells

decreased T cell number ( J:87435 )

• only moderate reductions in mature T cells in spleen and lymph nodes

decreased double-positive T cell number ( J:87435 )

• absolute numbers of double positive cells reduced

small lymph nodes ( J:87435 )

• lymph nodes reduced 50%

endocrine/exocrine glands

thymus hypoplasia ( J:87435 )

• thymic cellularity reduced to 1/8 normal

Genotype
MGI:3802921

cn55

• Allelic Composition: Fbxw7^tm1Iaai/Fbxw7^tm1Iaai; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129/Sv * C57BL/6

neoplasm

increased T cell derived lymphoma incidence ( J:137138 )

• 50% of mice develop T cell lymphomas later in life

endocrine/exocrine glands

increased T cell derived lymphoma incidence ( J:137138 )

• 50% of mice develop T cell lymphomas later in life

immune system

immune system phenotype ( J:137138 )

N • the numbers of double negative cells are normal

increased T cell derived lymphoma incidence ( J:137138 )

• 50% of mice develop T cell lymphomas later in life

hematopoietic system

increased T cell derived lymphoma incidence ( J:137138 )

• 50% of mice develop T cell lymphomas later in life

Genotype
MGI:3687234

cn56

• Allelic Composition: Mapk1^tm1Hed/Mapk1^tm1Hed; Mapk3^tm1Gpg/Mapk3^tm1Gpg; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129/Sv * C57BL/6 * DBA/2

immune system

abnormal T cell proliferation ( J:113280 )

• thymocytes display normal or slightly enhanced proliferation in vivo

small thymus ( J:113280 )

• some mice have very small thymuses (13 x 10⁶ cells) with few DN4 cells, but some have larger thymuses (115 x 10⁶ cells)

decreased thymocyte number ( J:113280 )

• total thymocyte number is decreased >3-fold compared to wild-type

increased double-positive T cell number ( J:113280 )

• mice have a substantial population of double-positive T cells

hematopoietic system

abnormal T cell proliferation ( J:113280 )

• thymocytes display normal or slightly enhanced proliferation in vivo

small thymus ( J:113280 )

• some mice have very small thymuses (13 x 10⁶ cells) with few DN4 cells, but some have larger thymuses (115 x 10⁶ cells)

decreased thymocyte number ( J:113280 )

• total thymocyte number is decreased >3-fold compared to wild-type

increased double-positive T cell number ( J:113280 )

• mice have a substantial population of double-positive T cells

endocrine/exocrine glands

small thymus ( J:113280 )

• some mice have very small thymuses (13 x 10⁶ cells) with few DN4 cells, but some have larger thymuses (115 x 10⁶ cells)

decreased thymocyte number ( J:113280 )

• total thymocyte number is decreased >3-fold compared to wild-type

cellular

abnormal T cell proliferation ( J:113280 )

• thymocytes display normal or slightly enhanced proliferation in vivo

Genotype
MGI:2684156

cn57

• Allelic Composition: Mcl1^tm2Sjk/Mcl1^tm3Sjk; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6 * DBA/2

hematopoietic system

increased T cell apoptosis ( J:86906 )

• increased apoptosis at double negative T cell stage

arrested T cell differentiation ( J:86906 )

• block at the double-negative stage between DN2 and DN3

decreased T cell number ( J:86906 )

• decreased number of peripheral T cells

• detected peripheral T cells did not undergo cre-mediated recombination

decreased thymocyte number ( J:86906 )

• reduction of the total number of thymocytes

decreased double-negative T cell number ( J:86906 )

• reduction of double-negative cells in DN3 and DN4 subsets, but not in DN1 or DN2 subsets

decreased double-positive T cell number ( J:86906 )

immune system

increased T cell apoptosis ( J:86906 )

• increased apoptosis at double negative T cell stage

arrested T cell differentiation ( J:86906 )

• block at the double-negative stage between DN2 and DN3

decreased T cell number ( J:86906 )

• decreased number of peripheral T cells

• detected peripheral T cells did not undergo cre-mediated recombination

decreased thymocyte number ( J:86906 )

• reduction of the total number of thymocytes

decreased double-negative T cell number ( J:86906 )

• reduction of double-negative cells in DN3 and DN4 subsets, but not in DN1 or DN2 subsets

decreased double-positive T cell number ( J:86906 )

cellular

increased T cell apoptosis ( J:86906 )

• increased apoptosis at double negative T cell stage

endocrine/exocrine glands

decreased thymocyte number ( J:86906 )

• reduction of the total number of thymocytes

Genotype
MGI:5691375

cn58

• Allelic Composition: Vdac2^tm1.1Ehyc/Vdac2^tm1.1Ehyc; Bax^tm2Sjk/Bax^tm2Sjk; Tg(Lck-cre)1Cwi/?
• Genetic Background: involves: 129X1/SvJ * C57BL/6 * DBA/2

immune system

small thymus ( J:220951 )

decreased thymocyte number ( J:220951 )

• two fold reduction of thymocytes although the developmental profile seems normal

hematopoietic system

small thymus ( J:220951 )

decreased thymocyte number ( J:220951 )

• two fold reduction of thymocytes although the developmental profile seems normal

endocrine/exocrine glands

small thymus ( J:220951 )

decreased thymocyte number ( J:220951 )

• two fold reduction of thymocytes although the developmental profile seems normal

Genotype
MGI:5691369

cn59

• Allelic Composition: Vdac2^tm1.1Ehyc/Vdac2^tm1.1Ehyc; Tg(Lck-cre)1Cwi/?
• Genetic Background: involves: 129X1/SvJ * C57BL/6 * DBA/2

immune system

abnormal thymocyte apoptosis ( J:220951 )

• BAK from T cells is more sensitive to apoptotic signals

• earlier translocation of cytochrome c out of mitochondria

• earlier caspase activation

• thymocytes cultured with antibody to CD3 die more rapidly

small thymus ( J:220951 )

• further shrinkage of thymus when mice injected with antibody to CD3

decreased thymocyte number ( J:220951 )

• two fold reduction of thymocytes although the developmental profile seems normal

• 90% of double+ thymocytes killed when mice injected with antibody to CD3

cellular

abnormal thymocyte apoptosis ( J:220951 )

• BAK from T cells is more sensitive to apoptotic signals

• earlier translocation of cytochrome c out of mitochondria

• earlier caspase activation

• thymocytes cultured with antibody to CD3 die more rapidly

hematopoietic system

abnormal thymocyte apoptosis ( J:220951 )

• BAK from T cells is more sensitive to apoptotic signals

• earlier translocation of cytochrome c out of mitochondria

• earlier caspase activation

• thymocytes cultured with antibody to CD3 die more rapidly

small thymus ( J:220951 )

• further shrinkage of thymus when mice injected with antibody to CD3

decreased thymocyte number ( J:220951 )

• two fold reduction of thymocytes although the developmental profile seems normal

• 90% of double+ thymocytes killed when mice injected with antibody to CD3

endocrine/exocrine glands

abnormal thymocyte apoptosis ( J:220951 )

• BAK from T cells is more sensitive to apoptotic signals

• earlier translocation of cytochrome c out of mitochondria

• earlier caspase activation

• thymocytes cultured with antibody to CD3 die more rapidly

small thymus ( J:220951 )

• further shrinkage of thymus when mice injected with antibody to CD3

decreased thymocyte number ( J:220951 )

• two fold reduction of thymocytes although the developmental profile seems normal

• 90% of double+ thymocytes killed when mice injected with antibody to CD3

Genotype
MGI:2683964

cn60

• Allelic Composition: Gata3^tm1Iho/Gata3^tm1Iho; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: BALB/c

mortality/aging

prenatal lethality, incomplete penetrance ( J:86996 )

• fewer than the expected numbers (6.5% vs 12.5%) of mutants are born, indicating partial prenatal lethality; time of lethality not determined

hematopoietic system

increased T cell apoptosis ( J:86996 )

• those double negative (DN) 3 cells that are able to develop into DN4 cells, exhibit increased apoptosis compared to controls

small thymus ( J:86996 )

decreased thymocyte number ( J:86996 )

• decrease in total thymocyte number at 4-6 weeks of age

• 10-fold reduction in the number of alpha/beta TCR+ thymocytes

decreased double-positive T cell number ( J:86996 )

arrested T cell differentiation ( J:86996 )

• double negative (DN) thymocytes fail to develop into large active double positive thymocytes

• accumulation of cells in the CD44-CD25+ DN3 stage and decreased CD44-CD25- DN4 cells, indicating a partial arrest at the DN3 to DN4 transition

abnormal T cell subpopulation ratio ( J:86996 )

• reduction in the CD4/CD8 ratio (1.33 vs 3-4 in controls)

decreased CD4-positive, alpha-beta T cell number ( J:86996 )

abnormal CD8-positive, alpha-beta T cell number ( J:86996 )

• variable numbers of total SP CD8 cell numbers, with a decrease in most cases but increases in other cases

immune system

increased T cell apoptosis ( J:86996 )

• those double negative (DN) 3 cells that are able to develop into DN4 cells, exhibit increased apoptosis compared to controls

small thymus ( J:86996 )

decreased thymocyte number ( J:86996 )

• decrease in total thymocyte number at 4-6 weeks of age

• 10-fold reduction in the number of alpha/beta TCR+ thymocytes

decreased double-positive T cell number ( J:86996 )

arrested T cell differentiation ( J:86996 )

• double negative (DN) thymocytes fail to develop into large active double positive thymocytes

• accumulation of cells in the CD44-CD25+ DN3 stage and decreased CD44-CD25- DN4 cells, indicating a partial arrest at the DN3 to DN4 transition

abnormal T cell subpopulation ratio ( J:86996 )

• reduction in the CD4/CD8 ratio (1.33 vs 3-4 in controls)

decreased CD4-positive, alpha-beta T cell number ( J:86996 )

abnormal CD8-positive, alpha-beta T cell number ( J:86996 )

• variable numbers of total SP CD8 cell numbers, with a decrease in most cases but increases in other cases

cellular

increased T cell apoptosis ( J:86996 )

• those double negative (DN) 3 cells that are able to develop into DN4 cells, exhibit increased apoptosis compared to controls

endocrine/exocrine glands

small thymus ( J:86996 )

decreased thymocyte number ( J:86996 )

• decrease in total thymocyte number at 4-6 weeks of age

• 10-fold reduction in the number of alpha/beta TCR+ thymocytes

Genotype
MGI:4417857

cn61

• Allelic Composition: Gata3^tm1Iho/Gata3^tm1Iho; Tg(DO11.10)10Dlo/0; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: BALB/c * C3H * C57BL/6

hematopoietic system

small thymus ( J:86996 )

decreased double-positive T cell number ( J:86996 )

• 10-fold reduction

arrested T cell differentiation ( J:86996 )

• partial impairment in generation of double positive cells

• partial arrest at the double negative 3 (DN3) to double negative 4 (DN4) transition

decreased CD4-positive, alpha-beta T cell number ( J:86996 )

• 10-fold reduction

decreased CD8-positive, alpha-beta T cell number ( J:86996 )

immune system

small thymus ( J:86996 )

decreased double-positive T cell number ( J:86996 )

• 10-fold reduction

arrested T cell differentiation ( J:86996 )

• partial impairment in generation of double positive cells

• partial arrest at the double negative 3 (DN3) to double negative 4 (DN4) transition

decreased CD4-positive, alpha-beta T cell number ( J:86996 )

• 10-fold reduction

decreased CD8-positive, alpha-beta T cell number ( J:86996 )

endocrine/exocrine glands

small thymus ( J:86996 )

Genotype
MGI:4355904

cn62

• Allelic Composition: Prdm1^tm2.1Nutt/Prdm1^tm2.1Nutt; Tg(Lck-cre)1Cwi/?
• Genetic Background: involves: C57BL/6

immune system

increased susceptibility to Orthomyxoviridae infection ( J:151858 )

• mice have some delay in recovering from an influenza infection compared to controls

• mice show pronounced lymphocytic accumulation in the tertiary lymphoid tissue after influenza infection compared to controls

Genotype
MGI:3775441

cn63

• Allelic Composition: Sh2d1a^tm2Vei/Sh2d1a^tm2Vei; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: C57BL/6

immune system

decreased germinal center B cell number ( J:131618 )

♀

decreased NK T cell number ( J:131618 )

♀ • the number of NK T cells is decreased 90% compared to in wild-type mice

decreased IgE level ( J:131618 )

♀ • in most mice following immunization with TNP-CGG

♀ • however, some mice could mount a partial antibody response

decreased IgG1 level ( J:131618 )

♀ • in most mice following immunization with TNP-CGG

♀ • however, some mice could mount a partial antibody response

decreased IgG2a level ( J:131618 )

♀ • in most mice following immunization with TNP-CGG

♀ • however, some mice could mount a partial antibody response

decreased IgG2b level ( J:131618 )

♀ • in most mice following immunization with TNP-CGG

♀ • however, some mice could mount a partial antibody response

decreased IgG3 level ( J:131618 )

♀ • in most mice following immunization with TNP-CGG

♀ • however, some mice could mount a partial antibody response

decreased IgM level ( J:131618 )

♀ • in most mice following immunization with TNP-CGG

♀ • however, some mice could mount a partial antibody response

hematopoietic system

decreased germinal center B cell number ( J:131618 )

♀

decreased NK T cell number ( J:131618 )

♀ • the number of NK T cells is decreased 90% compared to in wild-type mice

decreased IgE level ( J:131618 )

♀ • in most mice following immunization with TNP-CGG

♀ • however, some mice could mount a partial antibody response

decreased IgG1 level ( J:131618 )

♀ • in most mice following immunization with TNP-CGG

♀ • however, some mice could mount a partial antibody response

decreased IgG2a level ( J:131618 )

♀ • in most mice following immunization with TNP-CGG

♀ • however, some mice could mount a partial antibody response

decreased IgG2b level ( J:131618 )

♀ • in most mice following immunization with TNP-CGG

♀ • however, some mice could mount a partial antibody response

decreased IgG3 level ( J:131618 )

♀ • in most mice following immunization with TNP-CGG

♀ • however, some mice could mount a partial antibody response

decreased IgM level ( J:131618 )

♀ • in most mice following immunization with TNP-CGG

♀ • however, some mice could mount a partial antibody response

Genotype
MGI:5430375

cn64

• Allelic Composition: Taf7^tm1.1Dss/Taf7^tm1.2Dss; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: C57BL/6

immune system

small thymus ( J:185745 )

• very small

decreased thymocyte number ( J:185745 )

abnormal thymus physiology ( J:185745 )

• double negative thymocytes exhibit impaired proliferation

abnormal T cell differentiation ( J:185745 )

• mice exhibit defects in the transition from double negative to double positive thymocytes

increased double-negative T cell number ( J:185745 )

• slightly in the thymus

decreased double-positive T cell number ( J:185745 )

• in the thymus

decreased CD4-positive, alpha-beta T cell number ( J:185745 )

• in the thymus and spleen

decreased CD8-positive, alpha-beta T cell number ( J:185745 )

• in the thymus and spleen

hematopoietic system

small thymus ( J:185745 )

• very small

decreased thymocyte number ( J:185745 )

abnormal thymus physiology ( J:185745 )

• double negative thymocytes exhibit impaired proliferation

abnormal T cell differentiation ( J:185745 )

• mice exhibit defects in the transition from double negative to double positive thymocytes

increased double-negative T cell number ( J:185745 )

• slightly in the thymus

decreased double-positive T cell number ( J:185745 )

• in the thymus

decreased CD4-positive, alpha-beta T cell number ( J:185745 )

• in the thymus and spleen

decreased CD8-positive, alpha-beta T cell number ( J:185745 )

• in the thymus and spleen

endocrine/exocrine glands

small thymus ( J:185745 )

• very small

decreased thymocyte number ( J:185745 )

abnormal thymus physiology ( J:185745 )

• double negative thymocytes exhibit impaired proliferation

Genotype
MGI:4868731

cn65

• Allelic Composition: Hes1^tm1.1Frad/Hes1^tm1.1Frad; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: C57BL/6

immune system

immune system phenotype ( J:167000 )

N • normal T cell development

Genotype
MGI:3687233

cn66

• Allelic Composition: Mapk1^tm1.1Hed/Mapk1^tm1.1Hed; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: C57BL/6 * DBA/2

homeostasis/metabolism

abnormal enzyme/coenzyme level ( J:113280 )

• Mapk1 protein levels are decreased ~3-fold at the DN3 stage and from 25-100-fold at the DN4 stage

immune system

abnormal T cell subpopulation ratio ( J:113280 )

• there is a substantial increase in number of DN3 thymocytes over DN4, consistent with partial block in maturation

decreased CD4-positive, alpha-beta T cell number ( J:113280 )

• there is a larger decrease in CD4 T cells than CD8 T cells; ratio of CD4:CD8 goes down to 2.2 from 3.2 in wild-type

decreased CD8-positive, alpha-beta T cell number ( J:113280 )

• there is a decrease in CD8 T cells; ratio of CD4:CD8 goes down to 2.2 from 3.2 in wild-type

hematopoietic system

abnormal T cell subpopulation ratio ( J:113280 )

• there is a substantial increase in number of DN3 thymocytes over DN4, consistent with partial block in maturation

decreased CD4-positive, alpha-beta T cell number ( J:113280 )

• there is a larger decrease in CD4 T cells than CD8 T cells; ratio of CD4:CD8 goes down to 2.2 from 3.2 in wild-type

decreased CD8-positive, alpha-beta T cell number ( J:113280 )

• there is a decrease in CD8 T cells; ratio of CD4:CD8 goes down to 2.2 from 3.2 in wild-type

Genotype
MGI:3687232

cn67

• Allelic Composition: Mapk1^tm1Hed/Mapk1^tm1Hed; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: C57BL/6 * DBA/2

immune system

decreased T cell proliferation ( J:113280 )

• thymocytes display normal or slightly enhanced proliferation in vivo

decreased double-positive T cell number ( J:113280 )

• total number of double-positive (DP) thymocytes is reduced by 30%

abnormal T cell subpopulation ratio ( J:113280 )

• there is a substantial increase in number of DN3 thymocytes over DN4, consistent with partial block in maturation

hematopoietic system

decreased T cell proliferation ( J:113280 )

• thymocytes display normal or slightly enhanced proliferation in vivo

decreased double-positive T cell number ( J:113280 )

• total number of double-positive (DP) thymocytes is reduced by 30%

abnormal T cell subpopulation ratio ( J:113280 )

• there is a substantial increase in number of DN3 thymocytes over DN4, consistent with partial block in maturation

cellular

decreased T cell proliferation ( J:113280 )

• thymocytes display normal or slightly enhanced proliferation in vivo

Genotype
MGI:5582921

cn68

• Allelic Composition: Shcbp1^{tm1c(EUCOMM)Wtsi}/Shcbp1^{tm1c(EUCOMM)Wtsi}; Tg(Lck-cre)1Cwi/?
• Genetic Background: involves: C57BL/6 * DBA/2

immune system

immune system phenotype ( J:212898 )

N • in 2 week old mice that are not injected with MOG peptide, T cell development is normal

N • the fraction of absolute numbers of CD4+ and CD8+ T cells in spleen and lymph nodes is similar to controls

N • percentages and numbers of thymic compartment subsets in 4-6 week old mice are similar to controls

decreased susceptibility to experimental autoimmune encephalomyelitis ( J:212898 )

• reduced disease severity is observed in mice injected with MOG_35-55 peptide as compared to MOG-injected wild-type mice

Genotype
MGI:3578520

cn69

• Allelic Composition: Dicer1^tm1Mmk/Dicer1^tm1Mmk; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: C57BL/6 * DBA/2

immune system

increased gamma-delta T cell number ( J:98192 )

• unusually high percentage of thymocytes expressed TCR gamma-delta and gamma-delta cells were prevalent in the double negative compartment and decreased numbers of alpha-beta T cells

decreased T cell number ( J:98192 )

• decreased numbers of alpha-beta T cells

decreased thymocyte number ( J:98192 )

• cell numbers in thymi were reduced nearly 10-fold relative to controls, however there were normal numbers of double negative cells in thym

• increased percentage (43% versus 11% of wildtype at 6 hours and 68% versus 30% at 24 hours) of dying thymocytes when grown in vitro

hematopoietic system

increased gamma-delta T cell number ( J:98192 )

• unusually high percentage of thymocytes expressed TCR gamma-delta and gamma-delta cells were prevalent in the double negative compartment and decreased numbers of alpha-beta T cells

decreased T cell number ( J:98192 )

• decreased numbers of alpha-beta T cells

decreased thymocyte number ( J:98192 )

• cell numbers in thymi were reduced nearly 10-fold relative to controls, however there were normal numbers of double negative cells in thym

• increased percentage (43% versus 11% of wildtype at 6 hours and 68% versus 30% at 24 hours) of dying thymocytes when grown in vitro

endocrine/exocrine glands

decreased thymocyte number ( J:98192 )

• cell numbers in thymi were reduced nearly 10-fold relative to controls, however there were normal numbers of double negative cells in thym

• increased percentage (43% versus 11% of wildtype at 6 hours and 68% versus 30% at 24 hours) of dying thymocytes when grown in vitro

Genotype
MGI:5696657

cn70

• Allelic Composition: Nmt2^tm1.1Poru/Nmt2^tm1.1Poru; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: C57BL/6 * DBA/2 * SJL

immune system

decreased T cell number ( J:226234 )

• moderate in the thymus

• however, numbers are normal in the blood, thymus and spleen

decreased double-positive T cell number ( J:226234 )

• less so than in conditional Nmt1^tm1.1Poru and Nmt2^tm1.1Poru knock-out mice

decreased single-positive T cell number ( J:226234 )

• CD4+ and CD8+ single positive cells

hematopoietic system

decreased T cell number ( J:226234 )

• moderate in the thymus

• however, numbers are normal in the blood, thymus and spleen

decreased double-positive T cell number ( J:226234 )

• less so than in conditional Nmt1^tm1.1Poru and Nmt2^tm1.1Poru knock-out mice

decreased single-positive T cell number ( J:226234 )

• CD4+ and CD8+ single positive cells

Genotype
MGI:3687239

cn71

• Allelic Composition: Mapk1^tm1Hed/Mapk1^tm1Hed; Tg(Lck-cre)1Cwi/0; Tg(TcrAND)53Hed/0
• Genetic Background: involves: C57BL/6 * DBA/2 * SJL

immune system

abnormal positive T cell selection ( J:113280 )

• positive selection is diminished significantly

abnormal T cell subpopulation ratio ( J:113280 )

• CD4 to CD8 ratio (% of mature CD4 and CD8 T cells) is 20 compared to 257 in non transgenic littermates

hematopoietic system

abnormal positive T cell selection ( J:113280 )

• positive selection is diminished significantly

abnormal T cell subpopulation ratio ( J:113280 )

• CD4 to CD8 ratio (% of mature CD4 and CD8 T cells) is 20 compared to 257 in non transgenic littermates

Genotype
MGI:3653117

cn72

• Allelic Composition: Chd4^tm1.2Kge/Chd4^tm1.2Kge; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: C57BL/6 * DBA/2 * SJL

immune system

decreased T cell proliferation ( J:109547 )

• in vitro, TCR/CD3 stimulation shows a 5-7 fold decrease in proliferating T cells compared to controls, specifically CD8+CD4^lo and CD8+ CD4- populations

thymus hypoplasia ( J:109547 )

• null mice exhibit a consistent reduction in cellularity compared to wild-type or heterozygotes

abnormal T cell differentiation ( J:109547 )

increased double-negative T cell number ( J:109547 )

• there is an increase in the population of the T cells in the late double negative (DN4) stage in the neonatal thymus

decreased double-positive T cell number ( J:109547 )

• percentage of double positive thymocytes is severely reduced

increased CD8-positive, alpha-beta T cell number ( J:109547 )

• CD8+ cells are greatly increased in number

• CD8+ populations in the thymus express TCR levels more similar to double-positive cells rather than intermediate single-positive or single-positive levels

hematopoietic system

decreased T cell proliferation ( J:109547 )

• in vitro, TCR/CD3 stimulation shows a 5-7 fold decrease in proliferating T cells compared to controls, specifically CD8+CD4^lo and CD8+ CD4- populations

thymus hypoplasia ( J:109547 )

• null mice exhibit a consistent reduction in cellularity compared to wild-type or heterozygotes

abnormal T cell differentiation ( J:109547 )

increased double-negative T cell number ( J:109547 )

• there is an increase in the population of the T cells in the late double negative (DN4) stage in the neonatal thymus

decreased double-positive T cell number ( J:109547 )

• percentage of double positive thymocytes is severely reduced

increased CD8-positive, alpha-beta T cell number ( J:109547 )

• CD8+ cells are greatly increased in number

• CD8+ populations in the thymus express TCR levels more similar to double-positive cells rather than intermediate single-positive or single-positive levels

cellular

abnormal cell cycle ( J:109547 )

• activated T cells are unable to go through normal cell divisions

decreased T cell proliferation ( J:109547 )

• in vitro, TCR/CD3 stimulation shows a 5-7 fold decrease in proliferating T cells compared to controls, specifically CD8+CD4^lo and CD8+ CD4- populations

endocrine/exocrine glands

thymus hypoplasia ( J:109547 )

• null mice exhibit a consistent reduction in cellularity compared to wild-type or heterozygotes

Genotype
MGI:5696659

cn73

• Allelic Composition: Nmt1^tm1.1Poru/Nmt1^tm1.1Poru; Nmt2^tm1.1Poru/Nmt2^tm1.1Poru; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: C57BL/6 * DBA/2 * SJL

immune system

immune system phenotype ( J:226234 )

N • mice do not exhibit any signs of autoimmune disease

increased T cell apoptosis ( J:226234 )

• during all stages of T cell development except DN1 that does not express the cre transgene

• 10-fold in double positive cells

decreased T cell proliferation ( J:226234 )

• following treatment with CpG-ODNs, mice fail to exhibit an increased in CD4+ and CD8+ single positive T cells compared with wild-type mice

• however, untreated mice exhibit normal T cell proliferation and treated mice do exhibit an increase in macrophages in the spleen and liver as in wild-type mice

decreased splenocyte proliferation ( J:226234 )

• when stimulated with IL2

small thymus medulla ( J:226234 )

• however, cortical area is normal

increased double-negative T cell number ( J:226234 )

increased gamma-delta T cell number ( J:226234 )

• almost 3-fold

decreased T cell number ( J:226234 )

• in the thymus more so than single conditional knock-outs

• in the blood, lymph nodes and spleen as in Nmt1^tm1.1Poru conditional knock-outs

decreased DN4 thymocyte number ( J:226234 )

• at 6 months

decreased double-positive T cell number ( J:226234 )

• at 2 and 6 months more so than in either single conditional knock-out

decreased single-positive T cell number ( J:226234 )

• CD4+ and CD8+ single positive cells at 2 and 6 months

• following treatment with CpC-ODNs, mice fail to exhibit an increased in CD4+ and CD8+ single positive T cells compared with wild-type mice

abnormal CD4-positive, alpha beta T cell morphology ( J:226234 )

• CD4+ T cells isolated from the lymph nodes and spleen exhibit a shift from naive to activated/memory phenotypes

abnormal CD8-positive, alpha beta T cell morphology ( J:226234 )

• CD8+ T cells isolated from the lymph nodes and spleen exhibit a shift from naive to activated/memory phenotypes

decreased circulating interferon-gamma level ( J:226234 )

• in mice sensitized with D-galactosamine hydrochloride and treated with staphylococcal enterotoxin B

decreased circulating interleukin-2 level ( J:226234 )

• in mice sensitized with D-galactosamine hydrochloride and treated with staphylococcal enterotoxin B

decreased circulating tumor necrosis factor level ( J:226234 )

• in mice sensitized with D-galactosamine hydrochloride and treated with staphylococcal enterotoxin B

decreased interferon-gamma secretion ( J:226234 )

• after TCR-independent activation

decreased interleukin-2 secretion ( J:226234 )

• after TCR-independent activation

decreased susceptibility to endotoxin shock ( J:226234 )

• mice sensitized with D-galactosamine hydrochloride and treated with staphylococcal enterotoxin B exhibit reduced plasma levels of TNF, IL2 and IFN-gamma compared with wild-type mice

homeostasis/metabolism

decreased circulating interferon-gamma level ( J:226234 )

• in mice sensitized with D-galactosamine hydrochloride and treated with staphylococcal enterotoxin B

decreased circulating interleukin-2 level ( J:226234 )

• in mice sensitized with D-galactosamine hydrochloride and treated with staphylococcal enterotoxin B

decreased circulating tumor necrosis factor level ( J:226234 )

• in mice sensitized with D-galactosamine hydrochloride and treated with staphylococcal enterotoxin B

cellular

increased T cell apoptosis ( J:226234 )

• during all stages of T cell development except DN1 that does not express the cre transgene

• 10-fold in double positive cells

decreased T cell proliferation ( J:226234 )

• following treatment with CpG-ODNs, mice fail to exhibit an increased in CD4+ and CD8+ single positive T cells compared with wild-type mice

• however, untreated mice exhibit normal T cell proliferation and treated mice do exhibit an increase in macrophages in the spleen and liver as in wild-type mice

decreased splenocyte proliferation ( J:226234 )

• when stimulated with IL2

endocrine/exocrine glands

small thymus medulla ( J:226234 )

• however, cortical area is normal

decreased DN4 thymocyte number ( J:226234 )

• at 6 months

hematopoietic system

increased T cell apoptosis ( J:226234 )

• during all stages of T cell development except DN1 that does not express the cre transgene

• 10-fold in double positive cells

decreased T cell proliferation ( J:226234 )

• following treatment with CpG-ODNs, mice fail to exhibit an increased in CD4+ and CD8+ single positive T cells compared with wild-type mice

• however, untreated mice exhibit normal T cell proliferation and treated mice do exhibit an increase in macrophages in the spleen and liver as in wild-type mice

decreased splenocyte proliferation ( J:226234 )

• when stimulated with IL2

small thymus medulla ( J:226234 )

• however, cortical area is normal

increased double-negative T cell number ( J:226234 )

increased gamma-delta T cell number ( J:226234 )

• almost 3-fold

decreased T cell number ( J:226234 )

• in the thymus more so than single conditional knock-outs

• in the blood, lymph nodes and spleen as in Nmt1^tm1.1Poru conditional knock-outs

decreased DN4 thymocyte number ( J:226234 )

• at 6 months

decreased double-positive T cell number ( J:226234 )

• at 2 and 6 months more so than in either single conditional knock-out

decreased single-positive T cell number ( J:226234 )

• CD4+ and CD8+ single positive cells at 2 and 6 months

• following treatment with CpC-ODNs, mice fail to exhibit an increased in CD4+ and CD8+ single positive T cells compared with wild-type mice

abnormal CD4-positive, alpha beta T cell morphology ( J:226234 )

• CD4+ T cells isolated from the lymph nodes and spleen exhibit a shift from naive to activated/memory phenotypes

abnormal CD8-positive, alpha beta T cell morphology ( J:226234 )

• CD8+ T cells isolated from the lymph nodes and spleen exhibit a shift from naive to activated/memory phenotypes

Genotype
MGI:5696656

cn74

• Allelic Composition: Nmt1^tm1.1Poru/Nmt1^tm1.1Poru; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: C57BL/6 * DBA/2 * SJL

immune system

decreased T cell number ( J:226234 )

• moderate in the thymus

• in the blood, lymph nodes and spleen

decreased double-positive T cell number ( J:226234 )

• at 2 and 6 months, but less so than in conditional Nmt1^tm1.1Poru and Nmt2^tm1.1Poru knock-out mice

decreased single-positive T cell number ( J:226234 )

• CD4+ and CD8+ single positive cells

abnormal CD4-positive, alpha beta T cell morphology ( J:226234 )

• CD4+ T cells isolated from the lymph nodes and spleen exhibit a shift from naive to activated/memory phenotypes

abnormal CD8-positive, alpha beta T cell morphology ( J:226234 )

• CD8+ T cells isolated from the lymph nodes and spleen exhibit a shift from naive to activated/memory phenotypes

hematopoietic system

decreased T cell number ( J:226234 )

• moderate in the thymus

• in the blood, lymph nodes and spleen

decreased double-positive T cell number ( J:226234 )

• at 2 and 6 months, but less so than in conditional Nmt1^tm1.1Poru and Nmt2^tm1.1Poru knock-out mice

decreased single-positive T cell number ( J:226234 )

• CD4+ and CD8+ single positive cells

abnormal CD4-positive, alpha beta T cell morphology ( J:226234 )

• CD4+ T cells isolated from the lymph nodes and spleen exhibit a shift from naive to activated/memory phenotypes

abnormal CD8-positive, alpha beta T cell morphology ( J:226234 )

• CD8+ T cells isolated from the lymph nodes and spleen exhibit a shift from naive to activated/memory phenotypes

Genotype
MGI:3713382

cn75

• Allelic Composition: Chd4^tm1.1Kge/Chd4^tm1.2Kge; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: C57BL/6 * SJL

hematopoietic system

thymus hypoplasia ( J:109547 )

• reduced in mutant mice

immune system

thymus hypoplasia ( J:109547 )

• reduced in mutant mice

endocrine/exocrine glands

thymus hypoplasia ( J:109547 )

• reduced in mutant mice

Genotype
MGI:3713383

cn76

• Allelic Composition: Chd4^tm1.1Kge/Chd4^tm1.1Kge; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: C57BL/6 * SJL

immune system

decreased double-positive T cell number ( J:109547 )

• percentage of DP cells is reduced by 3-fold

arrested T cell differentiation ( J:109547 )

• differentiation of thymocytes stalls at the DN4 stage, but some do make the transition to DP eventually

• absolute number of double negative (DN) thymocytes is unaltered, or elevated despite decrease in thymus cellularity

increased CD8-positive, alpha-beta T cell number ( J:109547 )

• CD8 + T cells are increased to compensate for reduced DP cell numbers

abnormal CD4-positive, alpha beta T cell morphology ( J:109547 )

spleen hypoplasia ( J:109547 )

• 2- to 5-fold reduction in T cells in spleen is observed; decreased numbers of CD8+ and CD4+ populations is noted

abnormal T cell physiology ( J:109547 )

• DP and CD4+ T cells express lower levels of CD4, and CD8+ cells range from CD4^lo to CD4-

lymph node hypoplasia ( J:109547 )

• 2- to 5-fold reduction in T cells in lymph nodes is observed; decreased numbers of CD8+ and CD4+ populations is noted

hematopoietic system

decreased double-positive T cell number ( J:109547 )

• percentage of DP cells is reduced by 3-fold

arrested T cell differentiation ( J:109547 )

• differentiation of thymocytes stalls at the DN4 stage, but some do make the transition to DP eventually

• absolute number of double negative (DN) thymocytes is unaltered, or elevated despite decrease in thymus cellularity

increased CD8-positive, alpha-beta T cell number ( J:109547 )

• CD8 + T cells are increased to compensate for reduced DP cell numbers

abnormal CD4-positive, alpha beta T cell morphology ( J:109547 )

spleen hypoplasia ( J:109547 )

• 2- to 5-fold reduction in T cells in spleen is observed; decreased numbers of CD8+ and CD4+ populations is noted

abnormal T cell physiology ( J:109547 )

• DP and CD4+ T cells express lower levels of CD4, and CD8+ cells range from CD4^lo to CD4-

Genotype
MGI:3803970

cn77

• Allelic Composition: Akt1^tm1Pnt/Akt1^tm1Pnt; Akt3^tm1Mbb/Akt3^tm1Mbb; Tg(Lck-cre)1Cwi/0
• Genetic Background: Not Specified

hematopoietic system

thymus hypoplasia ( J:135903 )

• thymus cellularity is reduced by about 2.5 fold

decreased double-positive T cell number ( J:135903 )

• the percentage of double positive T cells in the thymus is reduced to 78.2% compared to 84.6% in wild-type mice

immune system

thymus hypoplasia ( J:135903 )

• thymus cellularity is reduced by about 2.5 fold

decreased double-positive T cell number ( J:135903 )

• the percentage of double positive T cells in the thymus is reduced to 78.2% compared to 84.6% in wild-type mice

endocrine/exocrine glands

thymus hypoplasia ( J:135903 )

• thymus cellularity is reduced by about 2.5 fold

Genotype
MGI:3803966

cn78

• Allelic Composition: Akt1^tm1Pnt/Akt1^tm1Pnt; Tg(Lck-cre)1Cwi/0
• Genetic Background: Not Specified

normal phenotype

no abnormal phenotype detected ( J:135903 )

• no abnormalities in T cell development are observed

Genotype
MGI:3830514

cn79

• Allelic Composition: Smarca4^tm1.2Pcn/Smarca4^tm1.2Pcn; Tg(Lck-cre)1Cwi/0; Tg(LCKprBCL2L1)12Sjk/0
• Genetic Background: Not Specified

immune system

abnormal T cell morphology ( J:144072 )

• 9% of the post-DN3 population is composed of CD4-DN4 cells compared to 25% in Smarca4^tm1Tich/Smarca4^tm1Tich Tg(Lck-cre)1Cwi Tg(LCKprBCL2L1)12Sjk mice

• 52% of the post-DN3 population is composed of CD4+DN4 cells compared to 39% in Smarca4^tm1Tich/Smarca4^tm1Tich Tg(Lck-cre)1Cwi Tg(LCKprBCL2L1)12Sjk mice

increased thymocyte number ( J:144072 )

• 8-fold compared to in Smarca4^tm1Gcr/Smarca4^tm1Tich Tg(Lck-cre)1Cwi mice

increased double-negative T cell number ( J:144072 )

• the DN2 population increases

• the DN3 population is rescued but is growth arrested

• the post-DN3 population is increased

decreased double-positive T cell number ( J:144072 )

• relative to DN4 cellularity

hematopoietic system

abnormal T cell morphology ( J:144072 )

• 9% of the post-DN3 population is composed of CD4-DN4 cells compared to 25% in Smarca4^tm1Tich/Smarca4^tm1Tich Tg(Lck-cre)1Cwi Tg(LCKprBCL2L1)12Sjk mice

• 52% of the post-DN3 population is composed of CD4+DN4 cells compared to 39% in Smarca4^tm1Tich/Smarca4^tm1Tich Tg(Lck-cre)1Cwi Tg(LCKprBCL2L1)12Sjk mice

increased thymocyte number ( J:144072 )

• 8-fold compared to in Smarca4^tm1Gcr/Smarca4^tm1Tich Tg(Lck-cre)1Cwi mice

increased double-negative T cell number ( J:144072 )

• the DN2 population increases

• the DN3 population is rescued but is growth arrested

• the post-DN3 population is increased

decreased double-positive T cell number ( J:144072 )

• relative to DN4 cellularity

endocrine/exocrine glands

increased thymocyte number ( J:144072 )

• 8-fold compared to in Smarca4^tm1Gcr/Smarca4^tm1Tich Tg(Lck-cre)1Cwi mice

Genotype
MGI:3830513

cn80

• Allelic Composition: Smarca4^tm1.2Pcn/Smarca4^tm1.2Pcn; Tg(Lck-cre)1Cwi/0
• Genetic Background: Not Specified

immune system

abnormal T cell morphology ( J:144072 )

• 14% of the post-DN3 population is composed of CD4+CD8- cells compared to 38% in Smarca4^tm1Tich/Smarca4^tm1Tich Tg(Lck-cre)1Cwi mice

• unlike in Smarca4^tm1Tich/Smarca4^tm1Tich Tg(Lck-cre)1Cwi mice, a CD4-CD8+ population analogous to immature single positive cells is observed

decreased double-negative T cell number ( J:144072 )

• post-DN3 cells are virtually absent

hematopoietic system

abnormal T cell morphology ( J:144072 )

• 14% of the post-DN3 population is composed of CD4+CD8- cells compared to 38% in Smarca4^tm1Tich/Smarca4^tm1Tich Tg(Lck-cre)1Cwi mice

• unlike in Smarca4^tm1Tich/Smarca4^tm1Tich Tg(Lck-cre)1Cwi mice, a CD4-CD8+ population analogous to immature single positive cells is observed

decreased double-negative T cell number ( J:144072 )

• post-DN3 cells are virtually absent

Genotype
MGI:5696658

tg81

• Allelic Composition: Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: C57BL/6 * DBA/2

immune system

decreased thymocyte number ( J:226234 )

endocrine/exocrine glands

decreased thymocyte number ( J:226234 )

hematopoietic system

decreased thymocyte number ( J:226234 )