Analysis Tools|
Allele Symbol Allele Name Allele ID |
Brca2tm1Kamc targeted mutation 1, Kimberly A McAllister MGI:2448890 |
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| Summary |
5 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• all mice die within 5 to 9 days of exposure to a dose at which 90% of wild-type mice survive
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• unlike in wild-type mice, wild-type transplanted cells engraft and reconstitute close to 45% after 6 months
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• colony forming cells are decreased 25% to 50% in the bone marrow and spleen of adult and 1.5 week old mice compared to in wild-type mice
• only 12% of bone marrow cells form large colonies compared to 45% of wild-type cells
• the reduction in colony forming cells is more marked as length of incubation in culture is increased
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• following whole body irradiation, mice exhibit leukopenia, anemia, and thrombopenia that are more severe and last longer than in similarly treated wild-type mice
• when subjected to a competitive repopulation assay, bone marrow cells exhibit reduced hematopoiesis compared to wild-type cells
• bone marrow cells maintained under physiological steady-state conditions exhibit impaired hematopoiesis compared to wild-type cells
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• femoral bone marrow cellularity is slightly reduced compared to in wild-type mice
• however, this decrease may reflect the decrease in body weight
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• spleen cellularity is slightly reduced compared to in wild-type mice
• however, this decrease may reflect the decrease in body weight
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• bone marrow cells exhibit a high number of spontaneous chromatid breaks compared to wild-type cells
• bone marrow cells exposed to 30 nM mitomycin C exhibit more chromatid breaks that similarly treated wild-type cells
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• colony forming cells are more sensitive to mitomycin C than wild-type cells
• however, dosage values for X-ray exposed cells is normal, and cells from mice transplanted with wild-type cells exhibit a partial rescue of mitomycin C sensitivity
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• no Rad51 foci are apparent in fibroblasts exposed to 12 Gy radiation unlike in similarly treated wild-type cells
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• by 15%
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• spleen cellularity is slightly reduced compared to in wild-type mice
• however, this decrease may reflect the decrease in body weight
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• no Rad51 foci are apparent in fibroblasts exposed to 12 Gy radiation unlike in similarly treated wild-type cells
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• all mice die within 5 to 9 days of exposure to a dose at which 90% of wild-type mice survive
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Mouse Models of Human Disease |
DO ID | OMIM ID(s) | Ref(s) | |
| Fanconi anemia complementation group D1 | DOID:0111089 |
OMIM:605724 |
J:144998 | |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• overall survivability significantly reduced
(J:75026)
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• 33% reduction in homozygotes at birth
• normal proportion of homozygotes at E13.5
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| N |
• no evidence of infertility
• no difficulty raising litters
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• increased incidence of a wide variety of solid tumors by 17 months
• tumor incidence more than 2X greater than in controls
• 61% of homozygotes develop tumors
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• dramatic lack of side branching
• lower density of ductules
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• dramatic lack of side branching
• lower density of ductules
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• 50% survival at 35 weeks of age
• 50% survival at 26 weeks when irradiated with 5Gy
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• increased incidence of gastric tumors
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• decreased latency for mammary tumor onset
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• increased incidence of osteosarcomas
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• increased incidence of osteosarcomas
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• decreased latency for mammary tumor onset
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• decreased latency for mammary tumor onset
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• increased incidence of gastric tumors
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• worse than in Swsap1em1Mjn homozygotes
• delayed at early zygonema, asynapsis at early pachynema
• fewer RAD51 and DMC1 foci at leptonema and early zygonema
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• slight
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• slight
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• worse than in Swsap1em1Mjn homozygotes
• delayed at early zygonema, asynapsis at early pachynema
• fewer RAD51 and DMC1 foci at leptonema and early zygonema
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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