Phenotypes associated with this allele

• Allele Symbol: Cenpe^tm1Dwc
• Allele Name: targeted mutation 1, Don W Cleveland
• Allele ID: MGI:2449007
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cenpe^tm1Dwc/Cenpe^tm1Dwc	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:2654560
cn2	Cenpe^tm1Dwc/Cenpe^tm2Dwc	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:3848814

Genotype
MGI:2654560

hm1

• Allelic Composition: Cenpe^tm1Dwc/Cenpe^tm1Dwc
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:81046 )

• mutants die before E7.5 but after E3.5

cellular

abnormal mitosis ( J:81046 )

• E3.5 embryos grown in culture exhibit aberrant mitosis, such as displaced chromosomes from the metaphase plate

abnormal inner cell mass proliferation ( J:81046 )

• E3.5 embryos grown in culture exhibit retarded growth of the inner cell mass

embryo

abnormal inner cell mass proliferation ( J:81046 )

• E3.5 embryos grown in culture exhibit retarded growth of the inner cell mass

Genotype
MGI:3848814

cn2

• Allelic Composition: Cenpe^tm1Dwc/Cenpe^tm2Dwc
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

cellular

abnormal mitosis ( J:81046 )

• fibroblasts infected with adenovirus encoding cre show mitotic abnormalities with defects in chromosome alignments; in some cases, one or two chromosomes are displaced from the metaphase plate, in other cases chromosomes fail to align or are clustered near the poles in a centrophilic position

• percentage of mitotic cells with misaligned chromosomes increases over time, from 28% at 24 hours postinfection with adenovirus encoding cre to 43% at 48 hours postinfection

• misaligned chromosomes result from a failure of chromosomes to congress, not from premature sister chromatid separation

• fibroblasts grown in vitro show a reduced number of kinetochore-associated microtubules