Analysis Tools
embryo
|
• homozygotes display abnormal positioning of the hindlimb bud
|
mortality/aging
|
• all homozygotes die within 24 hrs of birth
|
limbs/digits/tail
|
• homozygotes display abnormal positioning of the hindlimb bud
|
|
• homozygotes exhibit loss of caudal vertebrae
|
absent tail
(
J:81451
)
|
• at E15.5, homozygotes show absence of tail
|
nervous system
|
• homozygotes display increased olfactory epithelium neurogenesis, as shown by a significant increase in proliferating, neurogenin-1-expressing immediate neuronal precursors, and a ~20% increase in Ncam1-expressing olfactory receptor neurons
|
|
• at E14.5, homozygotes show a 37% increase in proliferating immediate neuronal precursors within the olfactory epithelium; this increase is more pronounced in the middle cell layers (147%)
|
|
• by E17.5, mutant retinas exhibit increased cell density in the ganglion cell layer (GCL); at P0, mutant GCLs contain ~50% more cells than wild-type GCLs
• excessive RGCs appear to differentiate normally, extending axons through the optic chiasm and tracts
• pulse labeling expts indicate that excessive RGCs accumulate as a result of prolonged production which occurs at the expense of amacrine and photoreceptor cells
• unlike the situation in the olfactory epithelium, mutant retinas display normal thickness as well as normal distribution and number of proliferating progenitor cells
|
|
• homozygotes exhibit a 37% increase in cross-sectional areas of optic nerves; the optic chiasm and tracts are abnormally thick
|
renal/urinary system
|
• at birth, homozygotes exhibit renal defects
|
skeleton
|
• homozygotes exhibit loss of caudal vertebrae
|
|
• at birth, all homozygotes display sacral agenesis
|
|
• homozygotes exhibit anterior transformations of vertebral segments
|
taste/olfaction
|
• homozygotes display a ~22% increase in thickness of the septal olfactory epithelium
|
|
• at E14.5, homozygotes show a 37% increase in proliferating immediate neuronal precursors within the olfactory epithelium; this increase is more pronounced in the middle cell layers (147%)
|
vision/eye
|
• by E17.5, mutant retinas exhibit increased cell density in the ganglion cell layer (GCL); at P0, mutant GCLs contain ~50% more cells than wild-type GCLs
• excessive RGCs appear to differentiate normally, extending axons through the optic chiasm and tracts
• pulse labeling expts indicate that excessive RGCs accumulate as a result of prolonged production which occurs at the expense of amacrine and photoreceptor cells
• unlike the situation in the olfactory epithelium, mutant retinas display normal thickness as well as normal distribution and number of proliferating progenitor cells
|
|
• homozygotes exhibit a 37% increase in cross-sectional areas of optic nerves; the optic chiasm and tracts are abnormally thick
|
|
• at E14.5, homozygotes show incomplete closure of the optic (choroid) fissure; in wild-type mice closure occurs at ~E12.5
• by E16.5, optic fissure closure is complete; subsequent formation of optic disc, optic nerve, and periocular tissue appears normal
|
|
• by E17.5, the inner plexiform layer is not detectable
|
respiratory system
|
• homozygotes display a ~22% increase in thickness of the septal olfactory epithelium
|
|
• at E14.5, homozygotes show a 37% increase in proliferating immediate neuronal precursors within the olfactory epithelium; this increase is more pronounced in the middle cell layers (147%)
|
craniofacial
|
• homozygotes display a ~22% increase in thickness of the septal olfactory epithelium
|
|
• at E14.5, homozygotes show a 37% increase in proliferating immediate neuronal precursors within the olfactory epithelium; this increase is more pronounced in the middle cell layers (147%)
|
cellular
|
• homozygotes display increased olfactory epithelium neurogenesis, as shown by a significant increase in proliferating, neurogenin-1-expressing immediate neuronal precursors, and a ~20% increase in Ncam1-expressing olfactory receptor neurons
|
growth/size/body
|
• homozygotes display a ~22% increase in thickness of the septal olfactory epithelium
|
|
• at E14.5, homozygotes show a 37% increase in proliferating immediate neuronal precursors within the olfactory epithelium; this increase is more pronounced in the middle cell layers (147%)
|
