Phenotypes associated with this allele

• Allele Symbol: Slc1a2^tm1Kta
• Allele Name: targeted mutation 1, Kohichi Tanaka
• Allele ID: MGI:2449707
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Slc1a2^tm1Kta/Slc1a2^tm1Kta	B6.129P2-Slc1a2^tm1Kta	MGI:4417920
hm2	Slc1a2^tm1Kta/Slc1a2^tm1Kta	involves: 129P2/OlaHsd * C57BL/6	MGI:3033260
ht3	Slc1a2^tm1Kta/Slc1a2^+	involves: 129P2/OlaHsd * C57BL/6	MGI:4838971
cx4	Slc1a2^tm1Kta/Slc1a2^+ Tg(SOD1*G93A)1Gur/0	involves: 129P2/OlaHsd * C57BL/6 * C57BL/6J * SJL/J	MGI:4838970

Genotype
MGI:4417920

hm1

• Allelic Composition: Slc1a2^tm1Kta/Slc1a2^tm1Kta
• Genetic Background: B6.129P2-Slc1a2^tm1Kta

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

postnatal lethality, complete penetrance ( J:124191 )

• mice die within 3 weeks of birth

Genotype
MGI:3033260

hm2

• Allelic Composition: Slc1a2^tm1Kta/Slc1a2^tm1Kta
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

mortality/aging

premature death ( J:40984 )

• only about 50% survive until after 6 weeks of age

behavior/neurological

increased susceptibility to pharmacologically induced seizures ( J:40984 )

• subconvulsive doses of pentylenetetrazole (PTZ) lead to high voltage sharp wave bursts

convulsive seizures ( J:40984 )

• explosive running followed by maintained opisthotonus and straub tail

• death usually followed within a few minutes of seizure

• survivors of a seizure resumed normal behavior but developed additional seizures later leading to death

growth/size/body

postnatal growth retardation ( J:40984 )

• although body weight was normal at birth, homozygous mice gained weight more slowly after birth

nervous system

increased susceptibility to pharmacologically induced seizures ( J:40984 )

• subconvulsive doses of pentylenetetrazole (PTZ) lead to high voltage sharp wave bursts

convulsive seizures ( J:40984 )

• explosive running followed by maintained opisthotonus and straub tail

• death usually followed within a few minutes of seizure

• survivors of a seizure resumed normal behavior but developed additional seizures later leading to death

cerebral edema ( J:40984 )

• 68% more brain edemas resulting from cold injury and size of edemas are much larger

abnormal telencephalon morphology ( J:40984 )

abnormal hippocampus CA1 region morphology ( J:40984 )

• selective neuronal degeneration in the hippocampal CA1 in mice aged 4-8 weeks

hippocampus pyramidal cell degeneration ( J:84859 )

• neuronal loss in the pyramidal layer of CA1 24 hours after brief (5 min) hippocampal ischemia leading to a build up of glutamate concentration

• by 4 days after ischemia, neuronal loss is seen throughout CA1 and CA3

• longer periods of ischemia are as damaging to wild-type mice as to homozygous mutants

homeostasis/metabolism

cerebral edema ( J:40984 )

• 68% more brain edemas resulting from cold injury and size of edemas are much larger

Genotype
MGI:4838971

ht3

• Allelic Composition: Slc1a2^tm1Kta/Slc1a2⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

cellular

abnormal cell physiology ( J:144898 )

• only have 65% glutamate transport when compared with wild-type mice in lumbar spinal cord and cerebral cortex

Genotype
MGI:4838970

cx4

• Allelic Composition: Slc1a2^tm1Kta/Slc1a2⁺; Tg(SOD1*G93A)1Gur/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * C57BL/6J * SJL/J

mortality/aging

premature death ( J:144898 )

• shortened lifespan (137 1.7 vs. 142 1.4 days)

cellular

abnormal cell physiology ( J:144898 )

• reduced glutamate transport in lumbar spinal cord at 120 days of age, compare with Tg(SOD1*G93A)1Gur mice

nervous system

decreased motor neuron number ( J:144898 )

• increase in the rate of motor decline accompanied by earlier motor neuron loss

• accelerated motor neuron loss at 120 days of age, compare with Tg(SOD1*G93A)1Gur mice

• significant declines occur between 105 and 120 days (42% reduction) and between 120 days and end stage, compared with between 120 days and endstage (56%) in Tg(SOD1*G93A)1Gur mice