Phenotypes associated with this allele

• Allele Symbol: Nr1d1^tm1Ven
• Allele Name: targeted mutation 1, Bjorn Vennstrom
• Allele ID: MGI:2449852
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Nr1d1^tm1Ven/Nr1d1^tm1Ven	B6.Cg-Nr1d1^tm1Ven	MGI:5908595
hm2	Nr1d1^tm1Ven/Nr1d1^tm1Ven	Not Specified	MGI:3815458

Genotype
MGI:5908595

hm1

• Allelic Composition: Nr1d1^tm1Ven/Nr1d1^tm1Ven
• Genetic Background: B6.Cg-Nr1d1^tm1Ven

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

decreased susceptibility to induced morbidity/mortality ( J:203422 )

• all mice exposed to cold between ZT4 and 10 survive unlike wild-type mice

adipose tissue

increased adipocyte glucose uptake ( J:203422 )

• during the day, but not night, in brown adipose tissue

homeostasis/metabolism

decreased susceptibility to induced hypothermia ( J:203422 )

• cold-exposed mice maintain their body temperature and all survive unlike control mice

increased body temperature ( J:203422 )

• in brown adipose tissue during the light phase in cold-exposed mice

increased core body temperature ( J:203422 )

• during the day, but not night, in brown adipose tissue

abnormal circadian temperature homeostasis ( J:203422 )

• increased core body temperature and brown adipose tissue during the light phase in cold-exposed mice

increased oxygen consumption ( J:203422 )

• in the brown adipose tissue of cold-exposed mice

cellular

increased adipocyte glucose uptake ( J:203422 )

• during the day, but not night, in brown adipose tissue

Genotype
MGI:3815458

hm2

• Allelic Composition: Nr1d1^tm1Ven/Nr1d1^tm1Ven
• Genetic Background: Not Specified

nervous system

abnormal cerebellum morphology ( J:60839 )

abnormal Purkinje cell differentiation ( J:60839 )

• Purkinje cells show a less elaborate dendritic tree during their differentiation period (P10-P16) and a delayed acquisition of Aldolase C expression pattern, indicating a delay in maturation

abnormal cerebellum development ( J:60839 )

• mutants exhibit delayed development of the cerebellum

abnormal cerebellum external granule cell layer morphology ( J:60839 )

• the disappearance of the external granule layer (EGL) is delayed, with mutants having a thicker EGL at P16

• the EGL between lobules VI-VII is thicker than between lobules IX-X at P16 and has more cell layers at P10 and P13

• granule cell progenitors are still proliferating at P16 in the EGL unlike in wild-type where no proliferation is seen at this time, indicating that precursor cell proliferation is extended by 3 days up to P16

abnormal Purkinje cell dendrite morphology ( J:60839 )

• the dendritic trees of Purkinje cells are less elaborate than in wild-type from P10 to P16 but appear normal after P19

abnormal cerebellar granule cell morphology ( J:60839 )

• mutants show an increase in granule neuronal apoptosis in the inner granule layer (IGL) at P10, P13, P16, and P19 compared to wild-type, but not in adulthood

abnormal cerebellar molecular layer ( J:60839 )

• the molecular layer shows a 15% and 17% decrease in thickness in lobules VI-VII and IX-X, respectively, at P16, however the size of the molecular layer is normal by P19

reproductive system

reduced female fertility ( J:60839 )

♀ • only 32% of matings with fertile males produce normal pregnancies compared to 75% for wild-type

growth/size/body

decreased body weight ( J:95758 )

muscle

increased skeletal muscle fiber size ( J:95758 )

• type I muscle fibers are twice as large in mutants as in wild-type extensor digitorum longus (EDL), however this is based on a very small sample size

abnormal skeletal muscle fiber type ratio ( J:95758 )

• the solelus muscle of mutants contains a higher amount of the beta/slow (type I) MyHC isoform than wild-type

cellular

abnormal Purkinje cell differentiation ( J:60839 )

• Purkinje cells show a less elaborate dendritic tree during their differentiation period (P10-P16) and a delayed acquisition of Aldolase C expression pattern, indicating a delay in maturation