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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Gzmatm1Simn
targeted mutation 1, Markus M Simon
MGI:2449926
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Gzmatm1Simn/Gzmatm1Simn B6.Cg-Gzmatm1Simn MGI:3525573
cx2
 		Gzmatm1Simn/Gzmatm1Simn
Gzmbtm1Ley/Gzmbtm1Ley 		B6.Cg-Gzmatm1Simn Gzmbtm1Ley MGI:3529788
cx3
 		Gzmatm1Simn/Gzmatm1Simn
Gzmbtm1Ley/Gzmbtm1Ley
Prf1tm1Sdz/Prf1tm1Sdz 		B6.Cg-Gzmatm1Simn Gzmbtm1Ley Prf1tm1Sdz MGI:3529809
cx4
 		Fastm1Osa/Fastm1Osa
Gzmatm1Simn/Gzmatm1Simn
Gzmbtm1Ley/Gzmbtm1Ley 		involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6 MGI:3589259
cx5
 		Gzmatm1Simn/Gzmatm1Simn
Gzmbtm1Ley/Gzmbtm1Ley 		involves: 129S2/SvPas * C57BL/6 MGI:3528062


Genotype
MGI:3525573
hm1
Allelic
Composition		 Gzmatm1Simn/Gzmatm1Simn
Genetic
Background		 B6.Cg-Gzmatm1Simn
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gzmatm1Simn mutation (8 available); any Gzma mutation (33 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
neoplasm
neoplasm ( J:28773 )
N
• homozygotes control growth of syngeneic tumors (s.c. inoculation of H-2b MC57G fibrosarcoma) as efficiently as wild-type mice

hematopoietic system
hematopoietic system phenotype ( J:28773 )
N
• homozygotes are viable, fertile, and display normal hematopoiesis relative to wild-type mice
• in mutant spleens, the numbers of CD4+ and CD8+ T cells, macrophages, NK cells and B cells fall within normal ranges
• the frequencies of CD4+ and CD8+ T cells responding to H-2 alloantigens in primary mixed lymphocyte cultures (MLC) with proliferation are also within normal ranges

immune system
immune system phenotype ( J:28773 , J:92718 )
N
• in vitro- and ex vivo-derived cytotoxic T cells and NK cells from mutant mice induce specific target cell membrane disruption, DNA fragmentation, and apoptosis as efficiently as wild-type cells (J:28773)
• homozygotes control infections with lymphocytic choriomeningitis (LCM) virus and L. monocytogenes as efficiently as wild-type mice (J:28773)
• homozygotes develop a normal contact hypersensitivity (CHS) response (based on increase in ear thickness) to trinitrophenyl (TNP) relative to wild-type mice (J:92718)




Genotype
MGI:3529788
cx2
Allelic
Composition		 Gzmatm1Simn/Gzmatm1Simn
Gzmbtm1Ley/Gzmbtm1Ley
Genetic
Background		 B6.Cg-Gzmatm1Simn Gzmbtm1Ley
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gzmatm1Simn mutation (8 available); any Gzma mutation (33 available)
Gzmbtm1Ley mutation (7 available); any Gzmb mutation (27 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
increased susceptibility to induced morbidity/mortality ( J:90852 )
• WNV-infected double homozygotes start to die at day 10 post-infection (p.i.); wild-type mice die between 10-13 days p.i.
• 70% of WNV-infected double homozygotes versus 29% of wild-type succumb to encephalitis by day 16 p.i.

immune system
immune system phenotype ( J:92718 )
N
• double homozygotes develop a normal contact hypersensitivity (CHS) response (based on increase in ear thickness) to trinitrophenyl (TNP) relative to wild-type mice
increased susceptibility to parasitic infection ( J:81397 )
• decreased clearance and survival after infection with Trypanosoma cruzi
increased susceptibility to Flaviviridae infection ( J:90852 )
• double homozygotes display increased susceptibility to West Nile flavivirus (WNV) infection relative to wild-type
• double mutants show a higher level of infectious virus in brain relative to wild-type or Prf1tm1Sdz mice at the same time point




Genotype
MGI:3529809
cx3
Allelic
Composition		 Gzmatm1Simn/Gzmatm1Simn
Gzmbtm1Ley/Gzmbtm1Ley
Prf1tm1Sdz/Prf1tm1Sdz
Genetic
Background		 B6.Cg-Gzmatm1Simn Gzmbtm1Ley Prf1tm1Sdz
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gzmatm1Simn mutation (8 available); any Gzma mutation (33 available)
Gzmbtm1Ley mutation (7 available); any Gzmb mutation (27 available)
Prf1tm1Sdz mutation (13 available); any Prf1 mutation (48 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
increased susceptibility to induced morbidity/mortality ( J:90852 )
• WNV-infected triple homozygotes display prolonged mean survival time (MST) but significantly increased mortality (50%) relative to either wild-type (29%) or Prf1tm1Sdz (18%) mice

immune system
immune system phenotype ( J:92718 )
N
• triple homozygotes develop a normal contact hypersensitivity (CHS) response (based on increase in ear thickness) to trinitrophenyl (TNP) relative to wild-type mice
increased susceptibility to parasitic infection ( J:81397 )
• decreased clearance and survival after infection with Trypanosoma cruzi
increased susceptibility to Flaviviridae infection ( J:90852 )
• triple homozygotes display increased susceptibility to West Nile flavivirus (WNV) infection relative to wild-type
• triple mutants show a higher level of infectious virus in brain relative to wild-type or Prf1tm1Sdz mice at the same time point




Genotype
MGI:3589259
cx4
Allelic
Composition		 Fastm1Osa/Fastm1Osa
Gzmatm1Simn/Gzmatm1Simn
Gzmbtm1Ley/Gzmbtm1Ley
Genetic
Background		 involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Fastm1Osa mutation (7 available); any Fas mutation (82 available)
Gzmatm1Simn mutation (8 available); any Gzma mutation (33 available)
Gzmbtm1Ley mutation (7 available); any Gzmb mutation (27 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
increased susceptibility to parasitic infection ( J:81397 )
• decreased clearance and survival after infection with Trypanosoma cruzi




Genotype
MGI:3528062
cx5
Allelic
Composition		 Gzmatm1Simn/Gzmatm1Simn
Gzmbtm1Ley/Gzmbtm1Ley
Genetic
Background		 involves: 129S2/SvPas * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gzmatm1Simn mutation (8 available); any Gzma mutation (33 available)
Gzmbtm1Ley mutation (7 available); any Gzmb mutation (27 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:44204 )
N
• in vitro- and ex vivo-derived cytotoxic T cells and NK cells from double mutant mice induce lysis in various target cells, at levels and with kinetics similar to those of single mutant or C57BL/6 mice
abnormal leukocyte physiology ( J:44204 )
• in vitro- and ex vivo-derived cytotoxic T cells and NK cells from double mutant mice fail to induce apoptotic nuclear damage in target cells
• in double mutants, Tc/NK-mediated target cell DNA fragmentation is undetectable, even after extended incubation periods (10 h)
• in contrast, Tc/NK-mediated target cell DNA fragmentation is normal in Gzmatm1Simn and only impaired in Gzmbtm1Ley mice in short-term (2-4 h), but not long-term (4-10 h), nucleolytic assays

hematopoietic system
abnormal leukocyte physiology ( J:44204 )
• in vitro- and ex vivo-derived cytotoxic T cells and NK cells from double mutant mice fail to induce apoptotic nuclear damage in target cells
• in double mutants, Tc/NK-mediated target cell DNA fragmentation is undetectable, even after extended incubation periods (10 h)
• in contrast, Tc/NK-mediated target cell DNA fragmentation is normal in Gzmatm1Simn and only impaired in Gzmbtm1Ley mice in short-term (2-4 h), but not long-term (4-10 h), nucleolytic assays




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
06/12/2024
MGI 6.13 		The Jackson Laboratory