Phenotypes associated with this allele

• Allele Symbol: Apc^tm3Mmt
• Allele Name: targeted mutation 3, Makoto M Taketo
• Allele ID: MGI:2449944
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Apc^tm3Mmt/Apc^tm3Mmt	involves: 129X1/SvJ * C57BL/6N	MGI:3606987
ht2	Apc^tm3Mmt/Apc^+	B6.129X1-Apc^tm3Mmt	MGI:3606986
ht3	Apc^tm3Mmt/Apc^+	involves: 129X1/SvJ * C57BL/6N	MGI:3811542
ht4	Apc^tm2Mmt/Apc^tm3Mmt	involves: 129X1/SvJ * C57BL/6N	MGI:3811543

Genotype
MGI:3606987

hm1

• Allelic Composition: Apc^tm3Mmt/Apc^tm3Mmt
• Genetic Background: involves: 129X1/SvJ * C57BL/6N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:81402 )

• embryos first appear abnormal at E7.5 and fail to turn by E9.5

• no homozygous mice born

embryo

abnormal developmental patterning ( J:81402 )

• at E8.5, an abnormal anterior region is displaced outside the yolk sac

• at E9.5, both the anterior and ventral regions appear abnormal

incomplete embryo turning ( J:81402 )

• unturned at E9.5

abnormal mesendoderm development ( J:81402 )

• impaired formation of axial mesendoderm

rostral body truncation ( J:81402 )

• anterior truncation at E8.5

rostral-caudal axis duplication ( J:81402 )

• partial axis duplication

absent neural folds ( J:81402 )

• head folds are missing

abnormal neural tube morphology ( J:81402 )

• at E9.5, the neural tube appears deformed

abnormal floor plate morphology ( J:81402 )

• floor plate is duplicated at the anterior end

split notochord ( J:81402 )

• notochord is duplicated at the anterior end

abnormal primitive node morphology ( J:81402 )

• at E7.5, the node is disorganized and expanded, leading to secondary axis formation

embryonic-extraembryonic boundary constriction ( J:81402 )

• constricted appearance at the border between embryonic and extraembryonic tissue at E7.5

abnormal anterior visceral endoderm morphology ( J:81402 )

• impaired formation of anterior visceral endoderm

nervous system

abnormal neural tube morphology ( J:81402 )

• at E9.5, the neural tube appears deformed

abnormal floor plate morphology ( J:81402 )

• floor plate is duplicated at the anterior end

abnormal brain development ( J:81402 )

• at E8.5, the brain is poorly formed, although some neural ectoderm is observed

• brain is truncated in the anterior hindbrain or caudal midbrain

abnormal hindbrain development ( J:81402 )

• part but not all of the hindbrain missing

absent midbrain ( J:81402 )

• missing midbrain

absent forebrain ( J:81402 )

• missing forebrain

cardiovascular system

abnormal endocardial heart tube morphology ( J:81402 )

• at E9.5, heart tubes remain on the dorsal side of the unturned embryo and fuse only partially

cardia bifida ( J:81402 )

• failure of the cardiac mesoderm to fuse at the ventral midline

digestive/alimentary system

abnormal foregut morphology ( J:81402 )

• foregut fails to form properly

growth/size/body

abnormal head development ( J:81402 )

• at E8.5, an abnormal anterior region (head) is displaced outside the yolk sac

Genotype
MGI:3606986

ht2

• Allelic Composition: Apc^tm3Mmt/Apc⁺
• Genetic Background: B6.129X1-Apc^tm3Mmt

digestive/alimentary system

increased intestinal adenoma incidence ( J:101604 )

• intesinal polyps are dysplastic adenomas

intestine polyps ( J:101604 )

• an average of 0.26 polyps per mouse at 15 months of age

neoplasm

increased intestinal adenoma incidence ( J:101604 )

• intesinal polyps are dysplastic adenomas

Genotype
MGI:3811542

ht3

• Allelic Composition: Apc^tm3Mmt/Apc⁺
• Genetic Background: involves: 129X1/SvJ * C57BL/6N

digestive/alimentary system

intestine polyps ( J:81402 )

• unlike Apc^tm1Mmt homozygotes, mice display few intestinal polyps

Genotype
MGI:3811543

ht4

• Allelic Composition: Apc^tm2Mmt/Apc^tm3Mmt
• Genetic Background: involves: 129X1/SvJ * C57BL/6N

embryo

abnormal embryonic tissue morphology ( J:81402 )

• at E7.5, the anterior embryonic region is protruding from the yolk sac; more prominent at E8.5

abnormal embryonic epiblast morphology ( J:81402 )

• at E6.5, embryonic ectoderm cells remain only in the distal region

• however, visceral endoderm develops normally

abnormal egg cylinder morphology ( J:81402 )

• at E6.5