Phenotypes associated with this allele

• Allele Symbol: Hprt1^{tm1(ACTB-Epor)Unc}
• Allele Name: targeted mutation 1, University of North Carolina
• Allele ID: MGI:2450106
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Hprt1^tm1(ACTB-Epor)Unc/Hprt1^tm1(ACTB-Epor)Unc	involves: 129P2/OlaHsd	MGI:2653305
ot2	Hprt1^tm1(ACTB-Epor)Unc/Y	involves: 129P2/OlaHsd	MGI:2653304

Genotype
MGI:2653305

hm1

• Allelic Composition: Hprt1^{tm1(ACTB-Epor)Unc}/Hprt1^{tm1(ACTB-Epor)Unc}
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

hematopoietic system phenotype ( J:81859 )

♀N • mutants appear normal, are healthy, breed well, and show no hematopoietic abnormalities under normal conditions

abnormal definitive hematopoiesis ( J:81859 )

♀ • mutants are phenotypically normal, but multipotent progenitor cells in the bone marrow show a proliferative response to exogenous erythropoietin not seen in wild-type bone marrow, showing an increase in total nucleated cells, myeloid cells and multipotent, clonogenic hematopoietic cells (CFU-S) and CFUs that give rise to granulocytes, erythroid cells, macrophages, and megakaryocytes within the same colony (CFU-GEMM) compared to wild-type

♀ • long term mutant bone marrow culture supplemented with erythropoietin shows prolonged and exponentially increasing hematopoiesis with continued accumulation of nonadherent cells long after controls have ceased proliferation in culture

Genotype
MGI:2653304

ot2

• Allelic Composition: Hprt1^{tm1(ACTB-Epor)Unc}/Y
• Genetic Background: involves: 129P2/OlaHsd

hematopoietic system

hematopoietic system phenotype ( J:81859 )

♂N • mutants appear normal, are healthy, breed well, and show no hematopoietic abnormalities under normal conditions

abnormal definitive hematopoiesis ( J:81859 )

♂ • mutants are phenotypically normal, but multipotent progenitor cells in the bone marrow show a proliferative response to exogenous erythropoietin not seen in wild-type bone marrow, showing an increase in total nucleated cells, myeloid cells and multipotent, clonogenic hematopoietic cells (CFU-S) and CFUs that give rise to granulocytes, erythroid cells, macrophages, and megakaryocytes within the same colony (CFU-GEMM) compared to wild-type

♂ • long term mutant bone marrow culture supplemented with erythropoietin shows prolonged and exponentially increasing hematopoiesis with continued accumulation of nonadherent cells long after controls have ceased proliferation in culture