Phenotypes associated with this allele

• Allele Symbol: Csnk2b^tm1.1Bb
• Allele Name: targeted mutation 1.1, Brigitte Boldyreff
• Allele ID: MGI:2450157
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Csnk2b^tm1.1Bb/Csnk2b^tm1.1Bb Tg(Gdf9-icre)5092Coo/0	involves: 129S2/SvPasCrl * C57BL/6	MGI:6845081
cn2	Csnk2b^tm1.1Bb/Csnk2b^tm1.2Bb Tg(Stra8-icre)1Reb/0	involves: 129S2/SvPasCrl * C57BL/6 * FVB/NJ	MGI:6845052
cn3	Csnk2b^tm1.1Bb/Csnk2b^tm1.1Bb Tg(ACTA1-cre)79Jme/?	involves: 129S2/SvPasCrl * C57BL/6J * SJL	MGI:3720797

Genotype
MGI:6845081

cn1

• Allelic Composition: Csnk2b^tm1.1Bb/Csnk2b^tm1.1Bb; Tg(Gdf9-icre)5092Coo/0
• Genetic Background: involves: 129S2/SvPasCrl * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

decreased oocyte number ( J:305036 )

♀

increased granulosa cell apoptosis ( J:305036 )

♀ • TUNEL analysis revealed increased granulosa cell apoptosis at 4 weeks of age

absent primary ovarian follicles ( J:305036 )

♀ • primary ovarian follicles are depleted at 8 weeks of age, with only a few MVH-positive primary follicles found scattered in the cortical region

♀ • however, number of primary follicles per ovary is normal at 3, 4 and 6 weeks of age

decreased primordial ovarian follicle number ( J:305036 )

♀ • number of primordial ovarian follicles per ovary is markedly reduced at 3, 4 and 6 weeks of age

absent primordial ovarian follicles ( J:305036 )

♀ • primordial ovarian follicles become depleted by 8 weeks of age, with only a few MVH-positive primordial follicles found scattered in the cortical region

decreased secondary ovarian follicle number ( J:305036 )

♀ • number of secondary follicles per ovary is markedly reduced at 6 weeks of age

absent secondary ovarian follicles ( J:305036 )

♀ • secondary ovarian follicles become depleted by 8 weeks of age

decreased tertiary ovarian follicle number ( J:305036 )

♀ • number of antral follicles per ovary is markedly reduced at 6 weeks of age

absent tertiary ovarian follicles ( J:305036 )

♀ • antral ovarian follicles become depleted by 8 weeks of age

increased atretic ovarian follicle number ( J:305036 )

♀ • although only a few follicles undergo atresia at 3 weeks, most follicles show signs of atresia by 4 weeks of age

♀ • massive atretic follicles are noted at 6 weeks of age (sexual maturity): oocytes in atretic follicles are eliminated and the space is filled with granulosa cells

♀ • nearly all types of follicles become depleted and no follicular structures are discernible in the ovaries by 8 weeks of age

decreased ovary weight ( J:305036 )

♀ • ovary weight to body weight ratio is significantly decreased at 4, 6, and 8 weeks of age

ovary atrophy ( J:305036 )

♀ • ovary size is only slightly smaller at 3 weeks but sharply decreased from 4 to 8 weeks of age

♀ • by 8 weeks, ovary size is reduced to about one fourth of that in control mice, indicating ovarian atrophy

premature ovarian failure ( J:305036 )

♀ • female infertility is attributed to premature ovarian failure caused by massive follicle atresia

female infertility ( J:305036 )

♀ • 6-8-week-old female mice fail to produce offspring during a 6-month mating period with wild-type males of known fertility

cellular

decreased oocyte number ( J:305036 )

♀

increased granulosa cell apoptosis ( J:305036 )

♀ • TUNEL analysis revealed increased granulosa cell apoptosis at 4 weeks of age

abnormal double-strand DNA break repair ( J:305036 )

♀ • at 2 weeks of age, small oocytes exhibit enhanced gammaH2AX signals indicative of accumulated unrepaired DSBs

♀ • by 4 weeks of age, the DNA damage response pathway is elevated, as indicated by upregulation of phosphorylated p53 (S15) in the ovaries

mortality/aging

premature ovarian failure ( J:305036 )

♀ • female infertility is attributed to premature ovarian failure caused by massive follicle atresia

endocrine/exocrine glands

increased granulosa cell apoptosis ( J:305036 )

♀ • TUNEL analysis revealed increased granulosa cell apoptosis at 4 weeks of age

absent primary ovarian follicles ( J:305036 )

♀ • primary ovarian follicles are depleted at 8 weeks of age, with only a few MVH-positive primary follicles found scattered in the cortical region

♀ • however, number of primary follicles per ovary is normal at 3, 4 and 6 weeks of age

decreased primordial ovarian follicle number ( J:305036 )

♀ • number of primordial ovarian follicles per ovary is markedly reduced at 3, 4 and 6 weeks of age

absent primordial ovarian follicles ( J:305036 )

♀ • primordial ovarian follicles become depleted by 8 weeks of age, with only a few MVH-positive primordial follicles found scattered in the cortical region

decreased secondary ovarian follicle number ( J:305036 )

♀ • number of secondary follicles per ovary is markedly reduced at 6 weeks of age

absent secondary ovarian follicles ( J:305036 )

♀ • secondary ovarian follicles become depleted by 8 weeks of age

decreased tertiary ovarian follicle number ( J:305036 )

♀ • number of antral follicles per ovary is markedly reduced at 6 weeks of age

absent tertiary ovarian follicles ( J:305036 )

♀ • antral ovarian follicles become depleted by 8 weeks of age

increased atretic ovarian follicle number ( J:305036 )

♀ • although only a few follicles undergo atresia at 3 weeks, most follicles show signs of atresia by 4 weeks of age

♀ • massive atretic follicles are noted at 6 weeks of age (sexual maturity): oocytes in atretic follicles are eliminated and the space is filled with granulosa cells

♀ • nearly all types of follicles become depleted and no follicular structures are discernible in the ovaries by 8 weeks of age

decreased ovary weight ( J:305036 )

♀ • ovary weight to body weight ratio is significantly decreased at 4, 6, and 8 weeks of age

ovary atrophy ( J:305036 )

♀ • ovary size is only slightly smaller at 3 weeks but sharply decreased from 4 to 8 weeks of age

♀ • by 8 weeks, ovary size is reduced to about one fourth of that in control mice, indicating ovarian atrophy

premature ovarian failure ( J:305036 )

♀ • female infertility is attributed to premature ovarian failure caused by massive follicle atresia

homeostasis/metabolism

abnormal double-strand DNA break repair ( J:305036 )

♀ • at 2 weeks of age, small oocytes exhibit enhanced gammaH2AX signals indicative of accumulated unrepaired DSBs

♀ • by 4 weeks of age, the DNA damage response pathway is elevated, as indicated by upregulation of phosphorylated p53 (S15) in the ovaries

Genotype
MGI:6845052

cn2

• Allelic Composition: Csnk2b^tm1.1Bb/Csnk2b^tm1.2Bb; Tg(Stra8-icre)1Reb/0
• Genetic Background: involves: 129S2/SvPasCrl * C57BL/6 * FVB/NJ

reproductive system

abnormal spermatid morphology ( J:289955 )

♂ • no round or elongated spermatids are detected in seminiferous tubules at 2 months of age

abnormal spermatocyte morphology ( J:289955 )

♂ • numbers of leptotene, zygotene and pachytene spermatocytes are significantly reduced in juvenile males

♂ • numbers of zygotene, pachytene and diplotene spermatocytes are significantly decreased at 2 months of age

♂ • however, numbers of spermatogonia and leptotene spermatocytes are normal at 2 months of age

decreased male germ cell number ( J:289955 )

♂ • number of germ cells per seminiferous tubule is significantly lower than that in controls at 12 dpp, 15 dpp and 17 dpp (corresponding to leptotene, zygotene and pachytene spermatocytes, respectively), and remains almost unchanged from 12 dpp to 17 dpp, unlike in control testes

♂ • number of MVH-positive germ cells is dramatically reduced at 2 months of age

azoospermia ( J:289955 )

♂ • no mature spermatozoa are present in the cauda epididymal lumens at 2 months of age

increased male germ cell apoptosis ( J:289955 )

♂ • number of TUNEL+ germ cells per seminiferous tubule is significantly increased at 2 months of age

increased testis apoptosis ( J:289955 )

♂ • more than 50% of the seminiferous tubules are TUNEL+ compared with less than 20% in control males

abnormal seminiferous tubule morphology ( J:289955 )

♂ • seminiferous tubules contain only a few spermatocytes at 12 dpp, 15 dpp and 17 dpp

♂ • only a few germ cells and no round or elongated spermatids are detected in seminiferous tubules at 2 months of age

small seminiferous tubules ( J:289955 )

♂ • seminiferous tubule diameter is reduced to half of that in control males at 2 months of age

small testis ( J:289955 )

♂ • testis size is markedly smaller at 2 months of age

decreased testis weight ( J:289955 )

♂ • testis weight-to-body weight ratio shows only small changes from day 12 to month 12 after birth and remains lower than that in controls at all ages examined

testicular atrophy ( J:289955 )

♂ • males develop testicular atrophy that does not change with increasing age after adulthood

arrest of spermatogenesis ( J:289955 )

♂ • first wave of spermatogenesis is severely blocked

male infertility ( J:289955 )

♂ • 8-week-old male mice fail to produce offspring during a 6-month mating period with wild-type females of known fertility

cellular

abnormal spermatid morphology ( J:289955 )

♂ • no round or elongated spermatids are detected in seminiferous tubules at 2 months of age

abnormal spermatocyte morphology ( J:289955 )

♂ • numbers of leptotene, zygotene and pachytene spermatocytes are significantly reduced in juvenile males

♂ • numbers of zygotene, pachytene and diplotene spermatocytes are significantly decreased at 2 months of age

♂ • however, numbers of spermatogonia and leptotene spermatocytes are normal at 2 months of age

decreased male germ cell number ( J:289955 )

♂ • number of germ cells per seminiferous tubule is significantly lower than that in controls at 12 dpp, 15 dpp and 17 dpp (corresponding to leptotene, zygotene and pachytene spermatocytes, respectively), and remains almost unchanged from 12 dpp to 17 dpp, unlike in control testes

♂ • number of MVH-positive germ cells is dramatically reduced at 2 months of age

azoospermia ( J:289955 )

♂ • no mature spermatozoa are present in the cauda epididymal lumens at 2 months of age

increased male germ cell apoptosis ( J:289955 )

♂ • number of TUNEL+ germ cells per seminiferous tubule is significantly increased at 2 months of age

increased testis apoptosis ( J:289955 )

♂ • more than 50% of the seminiferous tubules are TUNEL+ compared with less than 20% in control males

endocrine/exocrine glands

increased testis apoptosis ( J:289955 )

♂ • more than 50% of the seminiferous tubules are TUNEL+ compared with less than 20% in control males

abnormal seminiferous tubule morphology ( J:289955 )

♂ • seminiferous tubules contain only a few spermatocytes at 12 dpp, 15 dpp and 17 dpp

♂ • only a few germ cells and no round or elongated spermatids are detected in seminiferous tubules at 2 months of age

small seminiferous tubules ( J:289955 )

♂ • seminiferous tubule diameter is reduced to half of that in control males at 2 months of age

small testis ( J:289955 )

♂ • testis size is markedly smaller at 2 months of age

decreased testis weight ( J:289955 )

♂ • testis weight-to-body weight ratio shows only small changes from day 12 to month 12 after birth and remains lower than that in controls at all ages examined

testicular atrophy ( J:289955 )

♂ • males develop testicular atrophy that does not change with increasing age after adulthood

homeostasis/metabolism

abnormal protein level ( J:289955 )

♂ • Csnk2a2 mRNA expression and CSNK2A2 protein level is significantly decreased in testes

Genotype
MGI:3720797

cn3

• Allelic Composition: Csnk2b^tm1.1Bb/Csnk2b^tm1.1Bb; Tg(ACTA1-cre)79Jme/?
• Genetic Background: involves: 129S2/SvPasCrl * C57BL/6J * SJL

behavior/neurological

decreased grip strength ( J:110237 )

• after 4 months grip strength decreases as measured by the amount of time they could cling upside-down to a wire grid

nervous system

abnormal neuromuscular synapse morphology ( J:110237 )

• at 6 months, mice exhibit an impaired morphological appearance of the synaptic cholinergic receptor clusters whereby clusters are spotty

abnormal miniature endplate potential ( J:110237 )

• after 4 months, miniature endplate currents reduction accompanies reduction in grip strength

growth/size/body

decreased body weight ( J:110237 )

• at 4 months, body weight is slightly reduced by 5% to 10%