Analysis Tools
mortality/aging
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• homozygous mutant embryos die after implantation circa E10.5; no homozygotes are detected at E12.5
• the onset of embryonic lethality coincides with the maturation of mitochondria which initiate oxidative phosphorylation during this stage of embryogenesis
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nervous system
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• at E9.5 and E10.5, homozygotes display defective anterior neural tube closure
• however, normal closure of the posterior neural tube is observed
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exencephaly
(
J:81638
)
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• at E9.5 and E10.5, homozygotes display an open anterior neural tube
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cellular
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• at E10.5, mutant embryos display massively increased apoptosis relative to wild-type embryos, as visualized by anti-caspase-3 antibody staining
• however, mutant embryos develop normally until E9.5 without undergoing global apoptosis
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• mouse embryonic fibroblasts cultured from E9.5 homozygous mutant embryos (which do not yet respire aerobically) are not viable, indicating a role in cellular respiration
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embryo
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• at E10.5, mutant embryos display massively increased apoptosis relative to wild-type embryos, as visualized by anti-caspase-3 antibody staining
• however, mutant embryos develop normally until E9.5 without undergoing global apoptosis
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• at E10.5, any surviving mutant embryos are significantly smaller than wild-type embryos
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• at E9.5 and E10.5, homozygotes display defective anterior neural tube closure
• however, normal closure of the posterior neural tube is observed
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growth/size/body
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• at E10.5, any surviving mutant embryos are significantly smaller than wild-type embryos
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