Phenotypes associated with this allele

• Allele Symbol: Mep1b^tm1Bond
• Allele Name: targeted mutation 1, Judith S Bond
• Allele ID: MGI:2450870
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mep1b^tm1Bond/Mep1b^tm1Bond	B6.129-Mep1b^tm1Bond	MGI:3833919
hm2	Mep1b^tm1Bond/Mep1b^tm1Bond	involves: 129S1/Sv * 129X1/SvJ	MGI:2450872
hm3	Mep1b^tm1Bond/Mep1b^tm1Bond	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5439494

Genotype
MGI:3833919

hm1

• Allelic Composition: Mep1b^tm1Bond/Mep1b^tm1Bond
• Genetic Background: B6.129-Mep1b^tm1Bond

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:145037 )

N • similar blood urea nitrogen response to non-lethal ip injection with E. coli lipopolysaccharide to that seen in controls

N • similar circulating creatinine response to non-lethal ip injection with E. coli lipopolysaccharide to that seen in controls

N • similar hypothermia response to non-lethal ip injection with E. coli lipopolysaccharide to that seen in controls

abnormal blood homeostasis ( J:145037 )

• lipopolysaccharide induced initial elevation in nitrite/nitrate levels is similar to that of controls but more intense

immune system

immune system phenotype ( J:145037 )

N • proinflammatory cytokine response to non-lethal ip injection with E. coli lipopolysaccharide similar to that of controls

Genotype
MGI:2450872

hm2

• Allelic Composition: Mep1b^tm1Bond/Mep1b^tm1Bond
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

mortality/aging

prenatal lethality, incomplete penetrance ( J:81815 )

• partial embryonic lethality; reduced proportion (12%) of homozygous null F2 animals observed at birth

growth/size/body

increased body weight ( J:81815 )

• increased body weight at time of birth; females display increased body weight at time of weaning, but body weight was not statistically different at 150 days of age

reproductive system

decreased litter size ( J:81815 )

Genotype
MGI:5439494

hm3

• Allelic Composition: Mep1b^tm1Bond/Mep1b^tm1Bond
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

homeostasis/metabolism

decreased circulating creatinine level ( J:132021 )

♂ • at 6 to 24 hrs after kidney I/R injury, male homozygotes exhibit significantly lower plasma creatinine levels than similarly treated wild-type controls

decreased blood urea nitrogen level ( J:132021 )

♂ • at 6 to 72 hrs after kidney I/R injury, male homozygotes exhibit significantly lower BUN levels than similarly treated wild-type controls

abnormal urine homeostasis ( J:132021 )

♂ • at 6 and 24 hrs after kidney I/R injury, male homozygotes exhibit significantly lower urinary cytokine (CXCL1 and IL-6) levels relative to similarly treated wild-type controls

decreased susceptibility to kidney reperfusion injury ( J:132021 )

♂ • following kidney ischemia-reperfusion (I/R) injury, male homozygotes exhibit no death at 24-48 hrs post-injury, better preservation of renal function (as shown by lower BUN values at all time points), a less severe inflammatory response, and less disruption and shedding of membranes in proximal tubules cells relative to similarly treated wild-type controls

immune system

decreased inflammatory response ( J:132021 )

♂ • following kidney I/R injury, male homozygotes exhibit decreased leukocyte infiltration and reduced expression of inflammatory markers in the kidney relative to similarly treated wild-type controls

renal/urinary system

abnormal urine homeostasis ( J:132021 )

♂ • at 6 and 24 hrs after kidney I/R injury, male homozygotes exhibit significantly lower urinary cytokine (CXCL1 and IL-6) levels relative to similarly treated wild-type controls

decreased susceptibility to kidney reperfusion injury ( J:132021 )

♂ • following kidney ischemia-reperfusion (I/R) injury, male homozygotes exhibit no death at 24-48 hrs post-injury, better preservation of renal function (as shown by lower BUN values at all time points), a less severe inflammatory response, and less disruption and shedding of membranes in proximal tubules cells relative to similarly treated wild-type controls

proximal convoluted tubule brush border loss ( J:132021 )

♂ • at 96 hrs after kidney I/R injury, male homozygotes display significantly less brush-border disruption and collapse than wild-type controls, with less than 10% of tubules containing detached DPPIV staining material