Phenotypes associated with this allele

• Allele Symbol: Soat2^tm1Far
• Allele Name: targeted mutation 1, Bob Farese
• Allele ID: MGI:2450875
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Soat2^tm1Far/Soat2^tm1Far	involves: 129S4/SvJae * C57BL/6J	MGI:2450876
cx2	Apoe^tm1Unc/Apoe^tm1Unc Soat2^tm1Far/Soat2^tm1Far	involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6J	MGI:3762636

Genotype
MGI:2450876

hm1

• Allelic Composition: Soat2^tm1Far/Soat2^tm1Far
• Genetic Background: involves: 129S4/SvJae * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

decreased intestinal cholesterol absorption ( J:82032 )

• cholesterol absorption in homozygotes fed a high-fat (7.5%), high-cholesterol (1.25%) and 0.5% cholic acid diet is 85% lower than in wild-type

increased circulating triglyceride level ( J:82032 )

• homozygotes fed a high-fat (7.5%), high-cholesterol (1.25%) and 0.5% cholic acid diet or a chow diet exhibit elevated serum triglyceride level compared to wild-type fed the same diet; VLDL, IDL, and LDL fractions are increased

abnormal cholesterol homeostasis ( J:82032 )

• reduction in cholesterol ester synthesis in the small intestine and liver

increased circulating HDL cholesterol level ( J:82032 )

• mutants fed a western diet (20% fat and 0.15% cholesterol) exhibit increases in plasma HDL cholesterol

decreased circulating cholesterol level ( J:82032 )

• homozygotes exhibit resistance to diet-induced hypercholesterolemia; cholesterol in the VLDL, LDL and IDL fractions is reduced

decreased circulating LDL cholesterol level ( J:82032 )

• homozygotes fed a high-fat (7.5%), high-cholesterol (1.25%) and 0.5% cholic acid diet exhibit reduced cholesterol in the LDL fraction compared to wild-type

decreased circulating VLDL cholesterol level ( J:82032 )

• homozygotes fed a high-fat (7.5%), high-cholesterol (1.25%) and 0.5% cholic acid diet exhibit reduced cholesterol in the VLDL fraction compared to wild-type

liver/biliary system

decreased susceptibility to diet-induced hepatic steatosis ( J:82032 )

• when fed a high-fat (7.5%), high-cholesterol (1.25%) and 0.5% cholic acid diet, mutants do not develop fat-laden livers as seen in wild-type mice on the same diet

abnormal gallbladder physiology ( J:82032 )

• homozygotes exhibit resistance to diet-induced cholesterol gallstone formation

abnormal bile composition ( J:82032 )

• mutants on a high-fat (7.5%), high-cholesterol (1.25%) and 0.5% cholic acid diet exhibit bile cholesterol concentrations that are 30% lower than in wild-type mice

digestive/alimentary system

abnormal small intestine morphology ( J:82032 )

• free cholesterol levels are increased by 30% in the small intestine of mutants fed a high-fat (7.5%), high-cholesterol (1.25%) and 0.5% cholic acid diet

decreased intestinal cholesterol absorption ( J:82032 )

• cholesterol absorption in homozygotes fed a high-fat (7.5%), high-cholesterol (1.25%) and 0.5% cholic acid diet is 85% lower than in wild-type

Genotype
MGI:3762636

cx2

• Allelic Composition: Apoe^tm1Unc/Apoe^tm1Unc; Soat2^tm1Far/Soat2^tm1Far
• Genetic Background: involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6J

cardiovascular system

decreased susceptibility to atherosclerosis ( J:81836 )

• near absence of atherosclerotic lesions after 27 weeks on a CHOW diet

• near absence of atherosclerotic lesions after 9 weeks on a Western diet

homeostasis/metabolism

increased circulating HDL cholesterol level ( J:81836 )

• 58% increase in serum HDL cholesterol levels compared to Apoe^tm1Unc/Apoe^tm1Unc control mice

decreased circulating cholesterol level ( J:81836 )

• 60% reduction in total serum cholesterol compared to Apoe^tm1Unc/Apoe^tm1Unc control mice, primarily due to a reduction in plasma cholesterol esters

• plasma free cholesterol is reduced by 18% compared to Apoe^tm1Unc/Apoe^tm1Unc control mice

increased circulating triglyceride level ( J:81836 )

• 90% increase in total serum triglycerides compared to Apoe^tm1Unc/Apoe^tm1Unc control mice

increased phosphatidylcholine-sterol O-acyltransferase activity ( J:81836 )

• increase in the activity of the major enzyme responsible for synthesizing cholesterol esters in the plasma, lecithin/cholesterol acyltransferase