Phenotypes associated with this allele

• Allele Symbol: Pelo^tm1Imad
• Allele Name: targeted mutation 1, Ibrahim M Adham
• Allele ID: MGI:2450933
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pelo^tm1Imad/Pelo^tm1Imad	either: 129 or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6J)	MGI:3817220
ht2	Pelo^tm1Imad/Pelo^tm2Imad	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:5705410

Genotype
MGI:3817220

hm1

• Allelic Composition: Pelo^tm1Imad/Pelo^tm1Imad
• Genetic Background: either: 129 or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6J)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:81781 )

• normal number of deciduae at E6.5 and E7.5

• no homozygous embryos later than E8.5

• 28% of embryos abnormal at E7.5

• no development after E7.5

• by E8.5, all embryos are abnormally small or resorbed

embryo

absent inner cell mass proliferation ( J:81781 )

• cells derived from E3.5 embryos in culture cease proliferation after 4 days in culture

abnormal germ layer development ( J:81781 )

• three germ layers are identifiable but small

embryonic growth arrest ( J:81781 )

• no embryo development after E7.5

embryonic growth retardation ( J:81781 )

• smaller and developmentally retarded at E7.5

decreased embryo size ( J:228122 )

• E6.5 embryos are markedly smaller than heterozygous or wild-type controls

abnormal extraembryonic tissue morphology ( J:81781 )

• particularly reduced in size at E7.5

• trophoblast cells in culture continue growing after 4 days in culture

absent extraembryonic coelom ( J:81781 )

• lacks exocoelom at E7.5

abnormal extraembryonic endoderm formation ( J:228122 )

• extraembryonic endoderm forms and is found by immunohistology at E6.5, but appears not to be maintained in E7.5 embryos

cellular

aneuploidy ( J:81781 )

• observed in all developmentally delayed embryo

polyploidy ( J:81781 )

• observed in all developmentally delayed embryo

absent inner cell mass proliferation ( J:81781 )

• cells derived from E3.5 embryos in culture cease proliferation after 4 days in culture

growth/size/body

embryonic growth retardation ( J:81781 )

• smaller and developmentally retarded at E7.5

decreased embryo size ( J:228122 )

• E6.5 embryos are markedly smaller than heterozygous or wild-type controls

Genotype
MGI:5705410

ht2

• Allelic Composition: Pelo^tm1Imad/Pelo^tm2Imad
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

normal phenotype

no abnormal phenotype detected ( J:228122 )

• compound heterozygotes, having one functional allele, appear normal and are fertile