Phenotypes associated with this allele

• Allele Symbol: Ager^tm1.1Arnd
• Allele Name: targeted mutation 1.1, Bernd Arnold
• Allele ID: MGI:2451038
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ager^tm1.1Arnd/Ager^tm1.1Arnd	B6.129P2-Ager^tm1.1Arnd	MGI:3694553
hm2	Ager^tm1.1Arnd/Ager^tm1.1Arnd	involves: 129P2/OlaHsd	MGI:3696556
hm3	Ager^tm1.1Arnd/Ager^tm1.1Arnd	involves: 129P2/OlaHsd * C57BL/6	MGI:3694549

Genotype
MGI:3694553

hm1

• Allelic Composition: Ager^tm1.1Arnd/Ager^tm1.1Arnd
• Genetic Background: B6.129P2-Ager^tm1.1Arnd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

skeleton

decreased osteoclast cell number ( J:104998 )

• mutants have decreased numbers of osteoclasts per bone surface compared to wild-type

increased femur compact bone thickness ( J:104998 )

• males have increased cortical thickness at 3 months compared to wild-type males

increased bone mineral content ( J:104998 )

• at 1 and 3 months of age, males have significantly increased bone mineral content (BMC) compared to male wild-type, as seen in femora of males

increased bone mineral density of femur ( J:104998 )

• at 1 and 3 months of age, males have significantly increased bone mineral density (BMD) compared to male wild-type, as seen in femora of males

increased trabecular bone volume ( J:104998 )

• markedly higher trabecular bone volume in the proximal femur at 3 months

decreased bone trabecular spacing ( J:104998 )

• decreased trabecular spacing

increased trabecular bone thickness ( J:104998 )

• male mutants have increased trabecular thickness

increased bone mass ( J:104998 )

• increased bone mass is observed in males and females starting at 4 weeks of age

decreased bone resorption ( J:104998 )

• bone resorption is defective in ovariectomized (OVX) mutants; OVX mutants are resistant to bone loss unlike OVX wild-type mice

increased bone strength ( J:104998 )

• biomechanical bone strength and flexibility is increased in 3-month old mice compared to wild-type

limbs/digits/tail

increased femur compact bone thickness ( J:104998 )

• males have increased cortical thickness at 3 months compared to wild-type males

homeostasis/metabolism

decreased circulating interleukin-6 level ( J:104998 )

• mutants exhibit significantly lower serum IL-6 levels at 3 months of age, but not 1 month, compared to wild-type

immune system

decreased osteoclast cell number ( J:104998 )

• mutants have decreased numbers of osteoclasts per bone surface compared to wild-type

decreased circulating interleukin-6 level ( J:104998 )

• mutants exhibit significantly lower serum IL-6 levels at 3 months of age, but not 1 month, compared to wild-type

hematopoietic system

decreased osteoclast cell number ( J:104998 )

• mutants have decreased numbers of osteoclasts per bone surface compared to wild-type

Genotype
MGI:3696556

hm2

• Allelic Composition: Ager^tm1.1Arnd/Ager^tm1.1Arnd
• Genetic Background: involves: 129P2/OlaHsd

immune system

abnormal immune system physiology ( J:113376 )

• application of carboxymethyllysine-modified proteins (CML-mps) to rectosigmoid colon of mice does not activate NF kappa B in mutants or induce tissue inflammation whereas both are observed in treated wild-type mice

cellular

decreased fibroblast chemotaxis ( J:159873 )

• mouse embryonic fibroblasts (MEFs) exhibit impaired chemotaxis in response to HMGB1 compared with wild-type cells

• however, chemotaxis in response to PDGF is normal

Genotype
MGI:3694549

hm3

• Allelic Composition: Ager^tm1.1Arnd/Ager^tm1.1Arnd
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

homeostasis/metabolism

abnormal vascular wound healing ( J:82620 )

• mice show markedly decreased neointimal expansion after femoral artery denudation injury

increased circulating glucose level ( J:82491 )

• after streptozocin treatment, mutants and controls display serum glucose >250 mg/dl; >95% of mice develop diabetes

• after 12 weeks of disease, blood glucose in mutants measures 441 mg/dl, not significantly different from 473 mg/dl in diabetic controls

renal/urinary system

abnormal renal glomerulus basement membrane thickness ( J:82491 )

• after 12 weeks of diabetes in mutants, glomerular basement membrane thickness is increased ~3% compared to non-diabetic mutant controls while diabetic wild-type controls show ~14% increased thickness compared to non-diabetic wild-type controls

abnormal renal glomerulus morphology ( J:82491 )

• after 12 weeks, diabetic mutants display a ~5% increase in glomerular area compared to non-diabetic mutant controls, while diabetic wild-type controls exhibit an increase of ~18% in glomerular area

abnormal mesangial matrix morphology ( J:82491 )

• after 12 weeks, diabetic mutants display a 6% decrease in mesangial area compared to non-diabetic mutant controls, while diabetic wild-type controls exhibit an increases of ~61%

abnormal kidney weight ( J:82491 )

• diabetic mutant mice show no increase in kidney weight/body weight ratio after 12 weeks of disease whereas diabetic wild-type controls show a 1.6 fold increase in this parameter

cardiovascular system

abnormal vascular smooth muscle physiology ( J:82620 )

• VSM cell migration induced by calgranulin b is markedly decreased

decreased vascular smooth muscle cell proliferation ( J:82620 )

• VSM cell proliferation induced by calgranulin b is blunted

abnormal vascular wound healing ( J:82620 )

• mice show markedly decreased neointimal expansion after femoral artery denudation injury

muscle

abnormal vascular smooth muscle physiology ( J:82620 )

• VSM cell migration induced by calgranulin b is markedly decreased

decreased vascular smooth muscle cell proliferation ( J:82620 )

• VSM cell proliferation induced by calgranulin b is blunted

behavior/neurological

abnormal thermal nociception ( J:94702 )

• homozygous mice are partially protected from the increased nociceptive threshold caused by diabetes

cellular

decreased vascular smooth muscle cell proliferation ( J:82620 )

• VSM cell proliferation induced by calgranulin b is blunted